Toxicity of Trichloroethylene to
            <i>Pseudomonas putida</i>
            F1 Is Mediated by Toluene Dioxygenase

Wackett, Lawrence P.; Householder, Steven R.

doi:10.1128/aem.55.10.2723-2725.1989

Cited by 118 publications

(61 citation statements)

References 20 publications

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“…M declines [3]. The same phenomenon has been observed in other microorganisms that degrade TCE [4]. It has been suggested that the short half-life of TCE-degradation activity is due to enzyme inactivation by toxic intermediates formed by TCE degradation [5].…”

Section: Introductionsupporting

confidence: 65%

Induction and enhancement of stress proteins in a trichloroethylene-degrading methanotrophic bacterium, Methylocystis sp. M

Uchiyama

1999

FEMS Microbiology Letters

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The responses of the trichloroethylene-degrading bacterium Methylocystis sp. M to six different water-pollutants, carbon starvation, and temperature-shock (heat and cold) were examined using 2-dimensional gel electrophoresis. Twenty-eight polypeptides were induced, and these stress-induced proteins were classified into three groups. Some of the chemically induced proteins were the same as those induced by carbon starvation and temperature-shock. Two of the polypeptides were induced by trichloroethylene. Trichloroethylene-stress protein synthesis required 1^2 h at a concentration of trichloroethylene that had no effect on growth. Furthermore, 25 stress-enhanced polypeptides were observed, and one of these was enhanced by trichloroethylene. Based on these results, we discuss applications of chemical-stress induction of proteins to establish effective bioremediation and bioassay by methanotrophs. z

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Section: Introductionsupporting

confidence: 65%

Induction and enhancement of stress proteins in a trichloroethylene-degrading methanotrophic bacterium, Methylocystis sp. M

Uchiyama

1999

FEMS Microbiology Letters

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“…It is well known that enzymatic transformation of certain compounds can result in deleterious metabolic effects.2.1S, 18,28,34,42 However, the distinct mechanisms of inhibition and inactivation have not been modeled entirely satisfactorily to date. Competition for the primary enzyme of interest has been noted p r e v i o u~l y ,~~ and models have been proposed in which enzyme inactivation is expressed in terms of decreases in "active biomass.…”

Section: Discussionmentioning

confidence: 99%

A cometabilic kinetics model incorporating enzyme inhbition, inactivation, and recovery: I. Model development, analysis, and testing

Ely

Williamson

Guenther

et al. 1995

Biotech & Bioengineering

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Cometabolic biodegradation prcesses are important for bioremediation of hazardous waste sites. However, these proceeses are not well understood and have not been modeled thoroughly. Traditional Michaelis-Menten kinetics models often are used, but toxic effects and bacterial responses to toxicity may cause changes in enzyme levels, rendering such models inappropriate. In this article, a conceptual and mathematical model of cometabolic enzyme kinetics i described. Model derivation is based on enzyme/growth-substrate/nongrowth-substrate interaction and incorporates enzyme inhibition (caused by the presence of a cometabolic compound), inactivation (resulting from toxicity of a cometabolic product), and recovery (associated with bacterial synthesis of new enbzyme in response to inactivation). The mathematical model consists of a system of two, nonlinear ordinary differential equations that can be solved implicitly using numerical methods, providing estimates of model parameters. Model analysis shows that growth substraate adn nongrowth substrate oxidation rates are related by a dimensionless constant. Reliability of tehy model solution prcedure is verifiedl by abnalyzing data ses, containing random error, from simulated experimentss with trichhloroethyylene (TCE) degradation by ammonia-oxidizing bacterialunder various conditions. Estimation of the recovery rate contant is deterimined to be sensitive to intial TCE concentration. Model assumptions are evaluated in a companion article using data from TCE degradation experiments with amoniaoxidizing bacteria. (c) 1995 John Wiley & Sons, Inc.

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“…Highest purity methane ( s 99.99%, Matheson) was used for cell growth. [1,[2][3][4][5][6][7][8][9][10][11][12][13][14] C]TCE (99.8%) was obtained from New England Nuclear. Milli-Q water from a Corning Millipore D2 system was used for all experiments.…”

Section: Methodsmentioning

confidence: 99%

“…Anaerobic biotransformation of TCE proceeds through reductive dechlorination and produces the toxic agents dichloroethylene (DCE) and vinyl chloride (VC) [4,5]. Several aerobic bacteria including toluene oxidizers [6,7], ammonia oxidizers [8,9] and methane oxidizers [101 7] have also been shown to degrade TCE, but without the production of DCE or VC, and therefore can be useful alternatives. Since methane is non-toxic and methano-trophs are ubiquitous in the environment [18], these bacteria have been extensively examined for TCE bioremediation.…”

Section: Introductionmentioning

confidence: 99%

Identification of intermediates of in vivo trichloroethylene oxidation by the membrane-associated methane monooxygenase

Lontoh

2000

FEMS Microbiology Letters

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The rate and products of trichloroethylene (TCE) oxidation by Methylomicrobium album BG8 expressing membrane-associated methane monooxygenase (pMMO) were determined using 14 C radiotracer techniques. [ 14 C]TCE was degraded at a rate of 1.24 nmol (min mg protein)31 with the initial production of glyoxylate and then formate. Radiolabeled CO 2 was also found after incubating M. album BG8 for 5 h with [14 C]TCE. Experiments with purified pMMO from Methylococcus capsulatus Bath showed that TCE could be mineralized to CO 2 by pMMO. Oxygen uptake studies verified that M. album BG8 could oxidize glyoxylate and that pMMO was responsible for the oxidation based on acetylene inactivation studies. Here we propose a pathway of TCE oxidation by pMMO-expressing cells in which TCE is first converted to TCE-epoxide. The epoxide then spontaneously undergoes HCl elimination to form glyoxylate which can be further oxidized by pMMO to formate and CO 2 . ß

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Toxicity of Trichloroethylene to Pseudomonas putida F1 Is Mediated by Toluene Dioxygenase

Cited by 118 publications

References 20 publications

Induction and enhancement of stress proteins in a trichloroethylene-degrading methanotrophic bacterium, Methylocystis sp. M

Induction and enhancement of stress proteins in a trichloroethylene-degrading methanotrophic bacterium, Methylocystis sp. M

A cometabilic kinetics model incorporating enzyme inhbition, inactivation, and recovery: I. Model development, analysis, and testing

Identification of intermediates of in vivo trichloroethylene oxidation by the membrane-associated methane monooxygenase

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