“…Intensified antibiotic misuse events with accompanying bacterial resistance have drawn increasing global concern due to various adverse food safety and human health effects. , As a widely used broad-spectrum aminoglycoside antibiotic, gentamicin (GEN) with conspicuous effectiveness is extensively used in livestock production for treating infections of poultry and aquatic animals such as mastitis, pneumonia, and bacillary diarrhea. , Nonetheless, widespread negligence of proper withdrawal time led to residues in the environment exacerbating the emergence of superbugs, and potential accumulation in animal-derived food products aggravates the possibility of humans to suffer serious side effects (ototoxicity, nephrotoxicity, and vestibulotoxicity). − Based on these facts, it is therefore important to develop an efficient and reliable warning detection platform for reporting the fouling levels of Gen, thus ultimately safeguarding food safety and public health. From this motivation, Gen monitoring has advanced significantly from the microbiological method to analytical techniques, including capillary electrophoresis, HPLC/MS/MS, LC-MS/MS, and ic-ELISA . Although the analytical performance in aforementioned technologies is satisfactory, specialized staff, lengthy procedures, and cumbersome pretreatment fail to satisfy the ever-increasing requirements of on-site surveillance concerns, especially for the emergency cases in poor economies and developing countries .…”