Toxicity Studies of the Crude Aqueous Leaves Extracts of Ocimum gratissimum in Albino Rats

Ojo, Oluwafemi Adeleke

doi:10.9790/2402-0643439

Cited by 13 publications

(3 citation statements)

References 29 publications

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“…The Ocimum gratissimum leaves were dried and pulverized using a blender. The powder obtained weighing 70 g was macerated in 700 mL of distilled water for 24 h at room temperature as described by [32]. It was then filtered and the resulting filtrate was concentrated on a steam bath to give a yield of 40.45 g of the residue.…”

Section: Methodsmentioning

confidence: 99%

Ocimum gratissimum Linn. Leaves reduce the key enzymes activities relevant to erectile dysfunction in isolated penile and testicular tissues of rats

Ojo

Oyinloye

et al. 2019

BMC Complement Altern Med

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BackgroundOcimum gratissimum L. is a medicinal plant widely grown in tropical and subtropical regions with the leaf decoction usually taken in folk medicine to enhance erectile performance in men although the probable mechanism of actions remains undetermined. This study examined the inhibitory potentials of Ocimum gratissimum leaves on some key enzymes associated with erectile dysfunction in penile and testicular tissues of the rat.MethodsInhibitory effect of aqueous extract (1:10 w/v) of O. gratissimum leaves on the activities of phosphodiesterase-5 (PDE-5), arginase, angiotensin I –converting enzyme (ACE), and acetylcholinesterase (AChE) in penile and testicular tissues were assessed. Also, the extract was investigated for ferric reducing antioxidant property(FRAP) and 1,1-diphenyl-2-picryl-hydrazil (DPPH) radical scavenging abilities.ResultsThe extract showed higher PDE-5 (IC50 = 43.19 μg/mL), ACE (IC50 = 44.23 μg/mL), AChE (IC50 = 55.51 μg/mL) and arginase (IC50 = 46.12 μg/mL) inhibitory activity in the penile tissue than PDE-5 (IC50 = 44.67 μg/mL), ACE (IC50 = 53.99 μg/mL), AChE (IC50 = 60.03 μg/mL) and arginase (IC50 = 49.12 μg/mL) inhibitory activity in the testicular tissue homogenate. Furthermore, the extract scavenged free radicals and in a dose-dependent manner.ConclusionThe enzyme activities displayed might be associated with the bioactive compounds present in the extract which could possibly explain its use in the management of erectile dysfunction (ED).

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Section: Methodsmentioning

confidence: 99%

Ocimum gratissimum Linn. Leaves reduce the key enzymes activities relevant to erectile dysfunction in isolated penile and testicular tissues of rats

Ojo

Oyinloye

et al. 2019

BMC Complement Altern Med

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“…Similar findings have been reported in the literature. [18][19][20][21][22][23] The presence of alkaloids and other bioactive compounds invokes the medicinal characteristics of the plants and their therapeutic use in various pharmacopoeias. 24,25 Sub-acute toxicity, animal observation, body weight, food intake and water consumption measurement A sub-acute oral toxicity study in BALB/c mice indicated zero mortality after repeated VALAF administration for 21 days.…”

Section: Physicochemical and Phytochemical Analysismentioning

confidence: 99%

Sub-acute Toxicity Study of Bitter Leaf (Vernonia amygdalina Del.) Aqueous Fraction on Haematological Parameters

2024

TJNPR

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These activities are attributed to the established phytochemical components of the leaves, such as flavonoids, terpenes, polyphenols, glucosides, and steroidal saponins. 8 Several researchers have isolated and analysed some phytochemical substances with significant pharmacological effects from the leaves of V. amygdalina. Vernoniosides A, B, A1, A2, A3, B2, B3, A4 11 , sesquiterpene lactones, flavonoids such as luteolin 7-O-glucosides, luteolin 7-O-glucuronide, luteolin (12) and steroid glycosides are all examples of these compounds. 13 Understanding the safety of V. amygdalina in vivo is very important. This plant aqueous fraction toxicity has not yet been recorded. As a result, this study aimed to determine the sub-acute toxicity levels of the aqueous fraction of V. amygdalina leaves. Materials and Methods Plant materialsVernonia amygdalina Del was collected on March 2023 in Pauh,

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“…It was shown that aqueous extract of O. gratissimum ≥4.5 g/kg body weight could exert toxic effects in albino rats, whereas in lower doses used by traditional practitioners no toxicological effects threatened the liver (52). Another study demonstrated that the 30-day administration of 450 and 800 mg/kg body weight of aqueous extract had decreasing effects on RBC (red blood cell), PCV (packed cell volume), platelets and neutrophil count, and increased WBC (white blood cell) and lymphocyte count in albino Wistar rats (53).…”

Section: Ocimum Gratissimum L (Lamiaceae)mentioning

confidence: 99%

Medicinal Plants as Potential Hemostatic Agents

et al. 2020

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Purpose: Medicinal plants with a variety of phytochemical ingredients remain a potential source for new drug discovery. The use of medicinal herbs in a wide range of diseases and symptoms, such as bleeding, is prevalent in traditional and ethno medicine worldwide. Thus, this work provides a comprehensive review of medicinal plants or their isolated compounds, with respect to their ethno-medicinal use, which have demonstrated the stimulating effect on the hemostasis process. Methods: The relevant studies were withdrawn from electronic databases including Pubmed, EMBASE and Web of Science with a structured search methodology. Results: The total of 17 medicinal plants with hemostatic activity were extracted. The most frequently studied plant families were Compositae, Lamiaceae, Fabaceae, and Asteraceae. Bioactive compounds exerting hemostatic activity included tannins, iridoid glycosides, glycoconjugate, lignan, saponins and phenolic compounds. The most attributed mechanisms include coagulation stimulation via increasing the factor XII activity and plasma fibrinogen levels, the fibrinolysis inhibition, vascular or smooth muscle constriction and platelet aggregation. The most important adverse effects of high dose extract or isolated compounds administration were hepatotoxicity and nephrotoxicity. Conclusion: This review provides a list of medicinal plants with hemostatic activity that could be used as valuable sources of new plant-based hemostatic agents. Furthermore, this could be practical in detecting possible interactions of plants with anticoagulant, antiplatelet, fibrinolytic and antifibrinolytic medications. __________________________________________________________________________________________

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Toxicity Studies of the Crude Aqueous Leaves Extracts of Ocimum gratissimum in Albino Rats

Cited by 13 publications

References 29 publications

Ocimum gratissimum Linn. Leaves reduce the key enzymes activities relevant to erectile dysfunction in isolated penile and testicular tissues of rats

Ocimum gratissimum Linn. Leaves reduce the key enzymes activities relevant to erectile dysfunction in isolated penile and testicular tissues of rats

Sub-acute Toxicity Study of Bitter Leaf (Vernonia amygdalina Del.) Aqueous Fraction on Haematological Parameters

Medicinal Plants as Potential Hemostatic Agents

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