Toxicity testing of MWCNTs to aquatic organisms

Trompeta, Aikaterini-Flora; Preiss, Iris; Ben‐Ami, Frida; Benayahu, Yehuda; Charitidis, Costas A.

doi:10.1039/c9ra06672a

Cited by 26 publications

(9 citation statements)

References 44 publications

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“…Artemia salina is a zooplanktonic crustacean [25,26] and the nauplii of the brine shrimp are considered as a simple and suitable model system for acute toxicity tests [26,27]. The assay can be correlated with the toxicity data of rodents and humans and shows a good correlation with cytotoxicity tests, making these measurements suitable as preliminary results [26,28,29].…”

Section: In Vivo Toxicity Evaluation By Brine Shrimp Artemia Salinamentioning

confidence: 99%

Silver Nanoparticles from Oregano Leaves’ Extracts as Antimicrobial Components for Non-Infected Hydrogel Contact Lenses

Meretoudi

Banti

Raptis

et al. 2021

IJMS

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The oregano leaves’ extract (ORLE) was used for the formation of silver nanoparticles (AgNPs(ORLE)). ORLE and AgNPs(ORLE) (2 mg/mL) were dispersed in polymer hydrogels to give the pHEMA@ORLE_2 and pHEMA@AgNPs(ORLE)_2 using hydroxyethyl–methacrylate (HEMA). The materials were characterized by X-ray fluorescence (XRF) spectroscopy, X-ray powder diffraction analysis (XRPD), thermogravimetric differential thermal analysis (TG-DTA), derivative thermogravimetry/differential scanning calorimetry (DTG/DSC), ultraviolet (UV-Vis), and attenuated total reflection mode (ATR-FTIR) spectroscopies in solid state and UV–Vis in solution. The crystallite size value, analyzed with XRPD, was determined at 20 nm. The antimicrobial activity of the materials was investigated against Gram-negative bacterial strains Pseudomonas aeruginosa (P. aeruginosa) and Escherichia coli (E. coli). The Gram-positive ones of the genus of Staphylococcus epidermidis (S. epidermidis) and Staphylococcus aureus (S. aureus) are known to be involved in microbial keratitis by the means of inhibitory zone (IZ), minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC). The IZs, which developed upon incubation of P. aeruginosa, E. coli, S. epidermidis, and S. aureus with paper discs soaked in 2 mg/mL of AgNPs(ORLE), were 11.7 ± 0.7, 13.5 ± 1.9, 12.7 ± 1.7, and 14.3 ± 1.7 mm. When the same dose of ORLE was administrated, the IZs were 10.2 ± 0.7, 9.2 ± 0.5, 9.0 ± 0.0, and 9.0 ± 0.0 mm. The percent of bacterial viability when they were incubated over the polymeric hydrogel discs of pHEMA@AgNPs(ORLE)_2 was interestingly low (66.5, 88.3, 77.7, and 59.6%, respectively, against of P. aeruginosa, E. coli, S. epidermidis, and S. aureus) and those of pHEMA@ORLE_2 were 89.3, 88.1, 92.8, and 84.6%, respectively. Consequently, pHEMA@AgNPs(ORLE)_2 could be an efficient candidate toward the development of non-infectious contact lenses.

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Section: In Vivo Toxicity Evaluation By Brine Shrimp Artemia Salinamentioning

confidence: 99%

Silver Nanoparticles from Oregano Leaves’ Extracts as Antimicrobial Components for Non-Infected Hydrogel Contact Lenses

Meretoudi

Banti

Raptis

et al. 2021

IJMS

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“…In vivo toxicity of biomaterials on brine shrimp Artemia salina: A simple and a suitable model for the acute toxicity screening of materials, nanoparticles and metal complexes is the nauplii of the brine shrimp Artemia salina [5,8,15,[32][33][34][35][36]. Artemia salina is a zooplanktonic crustacean [32].…”

Section: Figure 10mentioning

confidence: 99%

Hydrogels containing water soluble conjugates of silver(i) ions with amino acids, metabolites or natural products for non infectious contact lenses

et al. 2021

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“…Nowadays, there is a growing concern about the possible effects of nanomaterials, as end of life products, on organisms and ecosystems [ 33 , 34 ]. Apart from studies investigating the mechanisms of interaction between CNTs and various biomacromolecules (DNA, RNA, etc.…”

Section: Introductionmentioning

confidence: 99%

Cytotoxicity Effects of Water-Soluble Multi-Walled Carbon Nanotubes Decorated with Quaternized Hyperbranched Poly(ethyleneimine) Derivatives on Autotrophic and Heterotrophic Gram-Negative Bacteria

et al. 2020

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Oxidized multi-walled carbon nanotubes (oxCNTs) were functionalized by a simple non-covalent modification procedure using quaternized hyperbranched poly(ethyleneimine) derivatives (QPEIs), with various quaternization degrees. Structural characterization of these hybrids using a variety of techniques, revealed the successful and homogenous anchoring of QPEIs on the oxCNTs’ surface. Moreover, these hybrids efficiently dispersed in aqueous media, forming dispersions with excellent aqueous stability for over 12 months. Their cytotoxicity effect was investigated on two types of gram(−) bacteria, an autotrophic (cyanobacterium Synechococcus sp. PCC 7942) and a heterotrophic (bacterium Escherichia coli). An enhanced, dose-dependent antibacterial and anti-cyanobacterial activity against both tested organisms was observed, increasing with the quaternization degree. Remarkably, in the photosynthetic bacteria it was shown that the hybrid materials affect their photosynthetic apparatus by selective inhibition of the Photosystem-I electron transport activity. Cytotoxicity studies on a human prostate carcinoma DU145 cell line and 3T3 mouse fibroblasts revealed that all hybrids exhibit high cytocompatibility in the concentration range, in which they also exhibit both high antibacterial and anti-cyanobacterial activity. Thus, QPEI-functionalized oxCNTs can be very attractive candidates as antibacterial and anti-cyanobacterial agents that can be used for potential applications in the disinfection industry, as well as for the control of harmful cyanobacterial blooms.

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Toxicity testing of MWCNTs to aquatic organisms

Abstract: Optical observation of Artemia salina′s intestine after 100 mg L−1 MWCNT-COOH exposure: the intestine appeared clean and restored to its normal appearance.

Cited by 26 publications

References 44 publications

Silver Nanoparticles from Oregano Leaves’ Extracts as Antimicrobial Components for Non-Infected Hydrogel Contact Lenses

Silver Nanoparticles from Oregano Leaves’ Extracts as Antimicrobial Components for Non-Infected Hydrogel Contact Lenses

Hydrogels containing water soluble conjugates of silver(i) ions with amino acids, metabolites or natural products for non infectious contact lenses

Cytotoxicity Effects of Water-Soluble Multi-Walled Carbon Nanotubes Decorated with Quaternized Hyperbranched Poly(ethyleneimine) Derivatives on Autotrophic and Heterotrophic Gram-Negative Bacteria

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