Toxigenicity of fungi from grain sorghum

Diener, U. L.; Morgan-Jones, G.; Wagener, R. E.; Davis, N. D.

doi:10.1007/bf00439062

Cited by 14 publications

(11 citation statements)

References 15 publications

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“…This may be probably attributed mainly to the bad storage condition. These results were coincided with those reported by many investigators Diener et al, 2 Gonzaleze et al, 3 Bhat et al Toxin production by some toxigenic fungi isolated from sorghum grains collected from different governorates…”

Section: Resultssupporting

confidence: 92%

“…Penicillium and Aspergillius were Alternaria, Penicillium sorghuma, Penicillium funiculosum and Aspergillus flavus, respectively. Diener et al, 2 Gonzalez et al 3 Outbreaks of aflatoxicosis in farm animals have been reported from many areas of the world. The liver is mainly affected in such outbreaks and also in experimental studies on animals, including nonhuman primates.…”

Section: Introductionmentioning

confidence: 99%

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Fungi and Mycotoxins Associated with Egyptian Sorghum Grains

Ma¹,

Salama²,

Khm³

et al. 2017

MOJT

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The aim of the present work was to study the infected fungi and the mycotoxins contamination in Egyptian sorghum grains collected from different governorates. Forty eight sorghum grain samples were collected from six governorates (Cairo, Kaliobia, Gharbia, Alexandaria, Assute, and Sohage). Isolation and identification of fungi and the determination of mycotoxins (i.e. Aflatoxins Afs and Fumonisin FB 1 ) were carried out. The results revealed that all samples were infected with at least one of fungi species. Nineteen fungi species belonging to five genera were isolated and identified from the investigated sorghum grain samples. Moreover, the result indicated that samples collected from Assute governorate were found to be more infected compared with the other governorates, meanwhile; samples collected from Kaliobia were found to be the lesser infected. All Aspergellus parasiticus and Fusarium moniliform isolated from sorghum grains were found to have the ability to produce Afs and FB 1 . In the same respect, results showed that aflatoxins were detected in 33.3 % (16 out of 48) of the investigated samples with levels ranged from 0.17µg/kg to 499µg/kg, while FB 1 was detected in 16.6 % (8 out of 48) of investigated samples with levels ranged from 7.2 to 129.5µg/kg. It is worthy to mention that the contamination levels of the investigated samples with aflatoxins were found below the Maximum Residue Limits MOJ Toxicology Research ArticleOpen Access Fungi and mycotoxins associated with egyptian sorghum grains 52Copyright:

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Section: Resultssupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Fungi and Mycotoxins Associated with Egyptian Sorghum Grains

Ma¹,

Salama²,

Khm³

et al. 2017

MOJT

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show abstract

“…Fungi causing deterioration have always been a major concern in cereals, such as sorghum, maize and paddy, because of their ability to produce mycotoxins. Fungi, especially those belonging to the genera Aspergillus and Fusarium , have been reported to occur at high incidence on cereal grains (Diener et al. 1981; Abdel‐Hafez et al.…”

Section: Discussionmentioning

confidence: 99%

“…Millions of people in the semi‐arid tropics of Asia and Africa depend on this crop for their staple diet. The quality of sorghum grains, particularly after wet weather in the early or late monsoon is often lowered, following colonization by fungi and with the subsequent accumulation of mycotoxins (Diener et al. 1981; Wilson et al.…”

Section: Introductionmentioning

confidence: 99%

PCR METHOD FOR THE DETECTION OF GENUS FUSARIUM AND FUMONISIN‐PRODUCING ISOLATES FROM FRESHLY HARVESTED SORGHUM GRAINS GROWN IN KARNATAKA, INDIA

Sreenivasa¹,

Dass

Raj

et al. 2008

Journal of Food Safety

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Sixty‐four isolates of Fusarium species isolated from 44 sorghum samples collected during 2004–2005 were subjected to polymerase chain reaction (PCR) analysis. PCR detection was performed on all Fusarium species using two different sets of primers, namely, internal transcribed spacer (ITS) and FUM1. The developed protocol is rapid for small‐scale extraction of DNA from fungal mycelia for the detection of Fusarium species using diagnostic PCR. Sixty‐four Fusarium isolates, namely, Fusarium verticillioides (45), Fusarium proliferatum (4), Fusarium anthophilum (4), Fusarium sporotrichioides (3), Fusarium pallidoroseum (6) and Fusarium oxysporum (2), were analyzed by PCR using the ITS and FUM1 set of primers. All Fusarium species scored positive with the ITS set of primers. Among the 64 isolates, 53 have scored positive with the FUM1 set of primers. These 53 isolates represent F. verticillioides (45), F. proliferatum (4) and F. anthophilum (4), respectively. The results of the study revealed that PCR‐based technique could be used to identify a group of potential fumonisin‐producing Fusarium species. PRACTICAL APPLICATIONS Polymerase chain reaction (PCR) method provides the basis for a simple, accurate, rapid and precise detection of potential fumonisin producing Fusarium species, which are of considerable risk to animal and human health. The detection of Fusarium species is therefore crucial for the prevention of toxins entering the food chain. The protocols developed in this study are helpful for a rapid and small‐scale extraction of nucleic acid from fungal mycelia, occurring on contaminated sorghum samples. A large number of samples can be screened in relatively less time using this PCR protocol when compared with the conventional methods.

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“…The presence of deteriorative fungi with ability to produce mycotoxin in grains and food represents a great hazard for human and animal health, and it has been reported for sorghum in many countries with a high frequency of Aspergillus and Fusarium genera (1,7,11,10,25).…”

Section: Introductionmentioning

confidence: 99%

Production of aflatoxins by Aspergillus flavus and of fumonisins by Fusarium species isolated from Brazilian sorghum

Silva¹,

Dilkin²,

Fonseca³

et al. 2004

Braz. J. Microbiol.

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Fifty-nine Aspergillus flavus and 35Fusarium verticillioides strains, isolated from freshly harvested (10) and stored (130) Brazilian sorghum samples, were tested regarding their ability to produce aflatoxins (coconut milk agar) and fumonisins (rice culture), respectively. Aflatoxins B1 and B2 were detected by TLC, and fumonisins B1 and B2 were analyzed by HPLC. Thirty-eight (64.4%) A. flavus strains produced detectable levels of aflatoxins at concentrations ranging from 12.00 to 3282.50 µg/kg (AFB1 + AFB2), while thirty two (91%) F. verticillioides strains produced FB1 at concentrations ranging from 0.12 to 5.38 µg/g. Two F. proliferatum strains produced low fumonisin levels. The toxigenic potential of A. flavus (64.4%) and F. verticillioides (91.5%) strains observed in sorghum samples indicates that rigorous control should be directed at the storage conditions of these products to minimize contamination with toxigenic deteriorating fungi, preventing further hazard to human and animal health.

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Toxigenicity of fungi from grain sorghum

Cited by 14 publications

References 15 publications

Fungi and Mycotoxins Associated with Egyptian Sorghum Grains

Fungi and Mycotoxins Associated with Egyptian Sorghum Grains

PCR METHOD FOR THE DETECTION OF GENUS FUSARIUM AND FUMONISIN‐PRODUCING ISOLATES FROM FRESHLY HARVESTED SORGHUM GRAINS GROWN IN KARNATAKA, INDIA

Production of aflatoxins by Aspergillus flavus and of fumonisins by Fusarium species isolated from Brazilian sorghum

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