Toxoplasma gondii inhibits Fas/CD95-triggered cell death by inducing aberrant processing and degradation of caspase 8

Vutova, Polya P.; Wirth, Martina; Hippe, Diana; Groß, Uwe; Schulze‐Osthoff, Klaus; Schmitz, Ingo; Lüder, Carsten G. K.

doi:10.1111/j.1462-5822.2007.00893.x

Cited by 67 publications

(59 citation statements)

References 52 publications

(92 reference statements)

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“…Although in type II cells (e.g., Jurkat, CEM) activation of Fas/CD95 leads to a weak formation of DISC, caspase 8 is still activated sufficiently to cleave the pro-apototic protein Bid, which induces release of cytochrome c from the mitochondria (see also below) [93,94]. In both type I and II cells, infection by T. gondii inhibits the apoptotic death receptor pathway by antagonizing caspase 8 ( Figure 2) [95]. Indeed, cells infected with T. gondii exhibit a lower total level of caspase 8 protein and activity, apparently via parasite-dependent proteolytic cleavage and degradation [95].…”

Section: Role Of the Death Receptor Pathwaymentioning

confidence: 99%

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Host cell manipulation by the human pathogen Toxoplasma gondii

Laliberté

Carruthers

2008

Cell. Mol. Life Sci.

161

123

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Toxoplasma gondii is an obligate intracellular parasite that can infect virtually any nucleated cell. During cell invasion Toxoplasma creates a subcellular compartment termed the parasitophorous vacuole, which acts as an interface between the parasite and host cytoplasm, and in many cases serves as a platform for modulation of several host cell functions that support parasite replication and infection. Spatial reorganization of host organelles and the remodeling of the cytoskeleton around the parasitophorous vacuole are observed early following entry and recent evidence suggests this interior redecorating promotes nutrient acquisition by the parasite. New findings also reveal that T. gondii manipulates signaling pathways of the host cell by deploying parasite kinases and a phosphatase, including at least two that infiltrate the host nucleus. T. gondii infection additionally controls several cellular pathways to establish an anti-apoptotic environment in a variety of cell types, and to subvert immune cells as a conduit for dissemination. In this review we discuss these recent developments in understanding how T. gondii achieves widespread success as a human and animal parasite by manipulating its host.

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Section: Role Of the Death Receptor Pathwaymentioning

confidence: 99%

“…In both type I and II cells, infection by T. gondii inhibits the apoptotic death receptor pathway by antagonizing caspase 8 ( Figure 2) [95]. Indeed, cells infected with T. gondii exhibit a lower total level of caspase 8 protein and activity, apparently via parasite-dependent proteolytic cleavage and degradation [95].…”

Section: Role Of the Death Receptor Pathwaymentioning

confidence: 99%

Host cell manipulation by the human pathogen Toxoplasma gondii

Laliberté

Carruthers

2008

Cell. Mol. Life Sci.

161

123

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“…In type I host cells (like SKW6.4 cells), T. gondii blocks this apoptotic cascade directly through interference with caspase 8. In type II host cells (like HeLa cells), T. gondii significantly reduces Fas/ CD95-triggered apoptosis by inhibiting the activities of initiator caspases 8 and 9 and effector caspase 3/7, by decreasing the apoptogenic function of mitochondria at the mitochondrial amplification loop (Vutova et al, 2007;Hippe et al, 2008).…”

Section: The Host Cell Is Exploited By T Gondii To Establish An Invamentioning

confidence: 99%

“…4, AUGUST 2011 cells is an anti-apoptosis-induced reaction triggered by T. gondii infection. Interference of T. gondii with host cell apoptosis is related to virulence factors of T. gondii strains (Angeloni et al, 2009) and depends on the cell types being infected (Vutova et al, 2007;Hippe et al, 2008). The mechanisms are mainly through regulating the death receptor (Hippe et al, 2008) and the NF-kB and the PI3K pathways (Yang et al, 2004).…”

Section: The Host Cell Is Exploited By T Gondii To Establish An Invamentioning

confidence: 99%

“…The mechanisms are mainly through regulating the death receptor (Hippe et al, 2008) and the NF-kB and the PI3K pathways (Yang et al, 2004). Toxoplasma gondii inhibits death receptor-mediated apoptosis in host cells (Goebel et al, 2001;Payne et al, 2003;Vutova et al, 2007). Fas/CD95 triggers an apoptotic cascade that is crucial for immunity and the outcome of infectious diseases.…”

Section: The Host Cell Is Exploited By T Gondii To Establish An Invamentioning

confidence: 99%

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A Review: Competence, Compromise, and Concomitance—Reaction of the Host Cell To Toxoplasma gondii Infection and Development

Peng¹,

Chen²,

Lindsay³

2011

Journal of Parasitology

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Modulation of Caspase Activation by Toxoplasma gondii

Hippe

Gais²,

Groß

et al. 2009

Methods in Molecular Biology

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Apoptosis plays crucial roles for the outcome of infection with various infectious agents. The host's apoptotic program may be modulated after infection in order to combat the pathogen or to restrict the immune response. In addition, distinct microorganisms alter the apoptotic program of the host in order to meet the requirements for their further distribution. The activation of caspases (i.e., cysteine proteases with specificity for aspartic acid residues) preludes the disassembly of the cell in response to apoptosis-inducing stimuli. This depends on the proteolytic cleavage of inactive proforms into catalytically active subunits. Analyses of the proteolysis and the enzymatic activity of caspases therefore represent valuable tools to study apoptotic programs during infection. The apicomplexan parasite Toxoplasma gondii interferes with the caspase cascade of its host cell in order to facilitate intracellular survival. The modulation of caspase activation by T. gondii is determined by SDS-PAGE and immunoblotting with caspase-specific antibodies. Furthermore, the impact of the parasite on caspase activity is fluorimetrically determined by measuring the cleavage of caspase-specific substrate analogues.

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Toxoplasma gondii inhibits Fas/CD95-triggered cell death by inducing aberrant processing and degradation of caspase 8

Cited by 67 publications

References 52 publications

Host cell manipulation by the human pathogen Toxoplasma gondii

Host cell manipulation by the human pathogen Toxoplasma gondii

A Review: Competence, Compromise, and Concomitance—Reaction of the Host Cell To Toxoplasma gondii Infection and Development

Modulation of Caspase Activation by Toxoplasma gondii

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