Toxoplasma gondii Recombinant Antigens in the Serodiagnosis of Toxoplasmosis in Domestic and Farm Animals

Ferra, Bartłomiej; Holec-Gąsior, Lucyna; Grąźlewska, Weronika

doi:10.3390/ani10081245

Cited by 20 publications

(25 citation statements)

References 111 publications

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“…At all infection doses, ROP4 VLP antigen showed higher IgG antibody responses compared to TLA antigen at 1, 2, 4, and 8 week PI, indicating that antibody induction from even a very low infectious dose can be detected using ROP4 VLP antigen. Importantly, we found that at higher infectious dosage (150 and 300 cysts), ROP4 VLP antigen showed higher IgG antibody responses as early as 1 week PI, which was not reported by using other recombinant proteins [ 12 ].…”

Section: Discussionmentioning

confidence: 62%

“…Sf9 cells were co-infected with the M1-rBVs and ROP4-rBVs to generate ROP4 VLPs. Afterward, VLPs were purified through a discontinuous sucrose gradient centrifugation and stored at −80°C until use as described previously [ 12 , 15 ].…”

Section: Methodsmentioning

confidence: 99%

“…Consequently, numerous studies have reported the acquisition of false-positive results along with poor specificity and sensitivity for T. gondii for this method [ 7 ]. In the past decade, multiple recombinant antigens with serodiagnostic potential were identified, which includes dense granule proteins (GRA) [ 8 – 10 ], the surface antigens (SAG) [ 5 , 11 ], microneme proteins (MIC), cyst matrix antigen (MAG1), apical membrane antigen (AMA) [ 12 ], and the rhoptry proteins (ROP) [ 13 , 14 ]. In the aforementioned studies, recombinant protein antigens were tested using the sera acquired from T. gondii -infected humans and animals.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Detection of Toxoplasma gondii Infections using Virus-Like Particles Displaying T. gondii ROP4 Antigen

Kim¹,

Mao²,

Kang³

et al. 2021

Korean J Parasitol

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Toxoplasma gondii ME49 infections are typically diagnosed by serological tests. However, serological diagnosis of RH strain-induced toxoplasmosis remains unknown. In order to develop seradiagnosis of above 2 kinds of infections, we generated recombinant virus-like particles (VLPs) displaying the T. gondii rhoptry protein 4 (ROP4) and evaluated their potential in T. gondii ME49 or RH strain infection diagnostics. Mice were orally infected with either the tachyzoites of T. gondii (RH) or cysts of T. gondii (ME49) at various dosages, and sera were collected at regular intervals. ELISA-based serological tests were performed to assess IgG, IgM, and IgA antibody responses against ROP4 VLP antigen and tissue lysate antigen (TLA). Compared to TLA, IgG, IgM, and IgA levels to ROP4 VLP antigen were significantly higher in the sera of T. gondii RH-infected mice 1 and 2 week post-infection (PI). T. gondii-specific IgG antibody was detected at 1, 2, 4, and 8 week PI in the T. gondii ME49-infected mice with infection dose-dependent manner. These results indicated that the ROP4 VLP antigen was highly sensitive antigens detecting T. gondii RH and ME49 antibodies at an early stage.

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Section: Discussionmentioning

confidence: 62%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Detection of Toxoplasma gondii Infections using Virus-Like Particles Displaying T. gondii ROP4 Antigen

Kim¹,

Mao²,

Kang³

et al. 2021

Korean J Parasitol

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“…As a final host, cats play an important role in spreading T. gondii infection [3]. Therefore, poor hygienic management of farms, climate, presence of cats in farms, consuming raw or uncooked meat and vegetables, and inter-current diseases may act as potential risk factors influencing this disease [4].…”

Section: Introductionmentioning

confidence: 99%

Molecular detection of Toxoplasma gondii in aborted fetuses of goats in Chattogram, Bangladesh

et al. 2021

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Background and Aim: Toxoplasma gondii is a protozoan parasite that is responsible for the major cause of congenital diseases, abortion, and stillbirth in humans and farm animals. Primary infection in pregnant goats due to T. gondii leads to abortion and significant economic losses in the livestock industry. Moreover, very few studies have been performed for the identification of T. gondii from aborted fetuses of goats. The study was conducted for the molecular identification of Toxoplasma gondii from aborted fetuses of goats in Chattogram, Bangladesh. Materials and Methods: Twenty aborted fetuses of goats were collected from 52 farms in the study area. A nested polymerase chain reaction (PCR) assay targeting the B1 gene was performed, and a positive sample yield of 197 bp amplified DNA products consistent with T. gondii. Results: The overall prevalence of toxoplasmosis in the aborted fetus of goats was 35.0%. Heart muscle, liver, brain, and placenta showed positive PCR results. The risk factors related to the does age, presence of cats in farms, and aborted fetus age were found to be statistically significant (p<0.05). Our results showed that T. gondii is a major possible causal factor for abortion and reproductive failure in goats. The high prevalence of T. gondii infection in aborted fetuses of goats revealed that T. gondii could be imperative in causing reproductive failure in goats. Conclusion: Active or congenital toxoplasmosis was shown by the presence of T. gondii in fetal tissues, which is a matter of concern as this parasite has zoonotic significance and causes economic hazards to the livestock industry by causing various reproductive problems. Therefore, proper control measures and strategies are needed to reduce the rate of abortion in goats, ultimately saving the livestock industry.

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“…Toxoplasmosis is caused by the protozoan parasite Toxoplasma gondii , where >60% of populations are infected with these parasites ( 1 , 2 ). The infection often occurs in areas of lower altitudes, hot and humid climates ( 2 ).…”

Section: Introductionmentioning

confidence: 99%

IFN‑γ and IL‑18 in conditioned media of parasite‑infected host and IL‑21‑silenced colorectal cancer cells

Ong

Abdalkareem

et al. 2020

Exp Ther Med

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The presence of certain soluble factors may provide a possible selective advantage for a parasite to gradually modify cell proliferation in neighbouring cells, which may result in chronic diseases. These soluble factors present in the conditioned medium also allow the parasite to invade rapidly into more host cells. The present study aimed to determine the levels of a group of type 1 T helper (Th1) cytokines in the conditioned media of host cells infected with parasites and in IL-21-silenced colorectal cancer cells. The conditioned media of human foreskin fibroblasts (HFFs) parasitized with the RH and ME49 strains of Toxoplasma gondii for 10 days were prepared, and subsequently the levels of the Th1 cytokines in the conditioned media were determined by ELISA. HFFs were incubated with the growth media containing selected soluble factors, and cell proliferation markers were subsequently analysed by reverse transcription-quantitative PCR. The mRNA expression level of cell proliferation markers was also examined in IL-21-silenced HCT116 cells, where the levels of soluble factors in the conditioned media were also determined as aforementioned. The results of the present study demonstrated that HFFs parasitized with ME49 released elevated levels of IFN-γ and lower levels of IL-18 into the conditioned medium compared with the controls. These phenomena were not observed in the conditioned medium of HFFs parasitized with RH. Similar levels of these soluble factors were also detected in the conditioned medium of IL-21-silenced HCT116 cells. The results of the present study also revealed that Ki67 and proliferating cell nuclear antigen mRNA expression was altered in host cells incubated with various levels of IFN-γ and IL-18, as well as in IL-21-silenced HCT116 cells compared with the respective controls. In conclusion, the current study provided preliminary evidence on the fundamental molecular mechanisms of host-parasite interactions that result in chronic diseases, which may aid in the treatment of these diseases in the relevant endemic regions.

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Toxoplasma gondii Recombinant Antigens in the Serodiagnosis of Toxoplasmosis in Domestic and Farm Animals

Cited by 20 publications

References 111 publications

Detection of Toxoplasma gondii Infections using Virus-Like Particles Displaying T. gondii ROP4 Antigen

Detection of Toxoplasma gondii Infections using Virus-Like Particles Displaying T. gondii ROP4 Antigen

Molecular detection of Toxoplasma gondii in aborted fetuses of goats in Chattogram, Bangladesh

IFN‑γ and IL‑18 in conditioned media of parasite‑infected host and IL‑21‑silenced colorectal cancer cells

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