Tp47 induces cell death involving autophagy and mTOR in human microglial HMO6 cells

Liu, Wen-Na; Jiang, Xiaoyong; JunRen,; Zhuang, Jingcong; Chen, Meihua; Zhu, Shao-Gang; Yang, Tian‐Ci; Yan, Jianghua

doi:10.1016/j.intimp.2019.04.013

Cited by 9 publications

(13 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previous studies have shown that Tp47 induced autophagy in human microglia cell line (HMO6) 9 . To examine whether Tp47 affects autophagy in HMC3 cells, the expression of several autophagy‐related proteins was measured.…”

Section: Resultsmentioning

confidence: 99%

“…Recombinant T. pallidum protein Tp47 was purified, and endotoxin was removed as described previously. 9,13,14 Endotoxin contamination in Tp47 was measured by a Tachypleus amebocyte lysate test (Chinese Horseshoe Crab Reagent Manufactory, Ltd., Xiamen, China) and was <0.05 endotoxin units (EUs)/mL.…”

Section: Preparation Of the Recombinant T Pallidum Protein Tp47mentioning

confidence: 99%

“…Previous studies have shown T. pallidum outer membrane protein Tp47 induced cell death through autophagy in human microglia HMO6 cell. 9 The HMO6 cell line and the human microglial clone 3 cell line (HMC3) are two different types of human microglia. HMC3 retains most of the phenotypic and morphological characteristics of the origin of primary microglia and is a classic cell model for studying neurological disease and phagocytosis.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Tp47 promoted the phagocytosis of HMC3 cells though autophagy induced by endoplamic reticlum stress

et al. 2022

Acad Dermatol Venereol

Self Cite

View full text Add to dashboard Cite

Background Central nervous system damage is an essential clinical feature that occurs in the early or late stages of syphilis infection. The abnormal enhancement of microglial phagocytosis can cause damage to the nervous system. However, the contribution of abnormally enhanced microglial phagocytosis to the pathogenesis of Treponema pallidum subsp. pallidum (T. pallidum) infection remains unknown. Objectives In this study, we sought to determine the role of recombinant T. pallidum Tp47 in promoting microglia phagocytosis and its associated mechanisms. Methods Microglial HMC3 cells were used to investigate the effect of the Tp47 on phagocytosis and the roles of autophagy and endoplasmic reticulum stress in Tp47‐induced phagocytosis. Results HMC3 cells exhibited obvious phagocytosis when stimulated with Tp47. The levels of P62 degradation, Beclin1 expression and the LC3II/LC3I ratio were significantly elevated, and the fusion of autophagosomes and lysosomes was promoted in Tp47‐stimulated HMC3 cells. Treatment with the autophagy inhibitors 3‐MA and Baf A1 inhibited Tp47‐induced phagocytosis. Meanwhile, the endoplasmic reticulum stress markers PERK, IRE1α, GRP78, ATF4 and XBP1s were upregulated in Tp47‐stimulated HMC3 cells. In addition, we found that TUDCA could inhibit Tp47‐induced expression of IRE1α but not PERK or ATF4. 4‐PBA inhibited TP47‐induced PERK and ATF4 protein expression but did not inhibit IRE1α expression. Attenuation of endoplasmic reticulum stress by administration of TUDCA and 4‐PBA abrogated Tp47‐mediated autophagy. Conclusions These results suggested that Tp47 activated autophagy through two key pathways associated with endoplasmic reticulum stress, PERK/ATF4 and IRE1/XBP1, to promote phagocytosis in HMC3 cells. These findings provided a basis for the understanding of the pathophysiology of neurological disorders that occur during the course of syphilis.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Preparation Of the Recombinant T Pallidum Protein Tp47mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Tp47 promoted the phagocytosis of HMC3 cells though autophagy induced by endoplamic reticlum stress

et al. 2022

Acad Dermatol Venereol

Self Cite

View full text Add to dashboard Cite

show abstract

“…The experimental group was stimulated with different concentrations of Tp47 (120, 300, 600, and 1200 ng/mL) based on previous studies. 28,30 Cells treated with 1 μg/mL lipopolysaccharide (LPS) or phosphate-buffered saline (PBS) were assigned to the positive control group or the negative control group, respectively. After 24 h of culturing, DCs in each group were collected for subsequent experiments.…”

Section: In Vitro Generation Of Dendritic Cellsmentioning

confidence: 99%

“…It has strong antigenicity and good specificity, mainly modulating the inflammation induced by T. pallidum. [27][28][29][30] Therefore, we chose Tp47 to induce a T. pallidum infection model in this study.…”

Section: Introductionmentioning

confidence: 99%

The role of the 47-kDa membrane lipoprotein of Treponema pallidum in promoting maturation of peripheral blood monocyte-derived dendritic cells without enhancing C-C chemokine receptor type 7-mediated dendritic cell migration

Cheng¹,

Lu²,

Chen³

et al. 2022

Adv Clin Exp Med

View full text Add to dashboard Cite

show abstract

Refining a non-invasive prediction model for neurosyphilis diagnosis by using immunoassay to detect serum anti-TP0435 (TP17) and TP0574 (TP47) IgG antibodies: two-centre cross-sectional retrospective study in China

Ke,

Ao,

Wang

et al. 2024

Clinical Microbiology and Infection

View full text Add to dashboard Cite

Tp47 induces cell death involving autophagy and mTOR in human microglial HMO6 cells

Cited by 9 publications

References 32 publications

Tp47 promoted the phagocytosis of HMC3 cells though autophagy induced by endoplamic reticlum stress

Tp47 promoted the phagocytosis of HMC3 cells though autophagy induced by endoplamic reticlum stress

The role of the 47-kDa membrane lipoprotein of Treponema pallidum in promoting maturation of peripheral blood monocyte-derived dendritic cells without enhancing C-C chemokine receptor type 7-mediated dendritic cell migration

Refining a non-invasive prediction model for neurosyphilis diagnosis by using immunoassay to detect serum anti-TP0435 (TP17) and TP0574 (TP47) IgG antibodies: two-centre cross-sectional retrospective study in China

Contact Info

Product

Resources

About