TP53 Tumor Suppressor Protein in Normal Human Fibroblasts Does Not Respond to 837 MHz Microwave Exposure

Abstract

The TP53 tumor suppressor protein (formerly known as p53) responds to a wide variety of environmental insults. To evaluate the safety of cellular telephones, TP53 responses in human fibroblast cells were studied after exposure to 837 MHz microwaves. Cells were exposed in a temperature-controlled transverse electromagnetic (TEM) chamber to a specific absorption rate (SAR) of 0.9 or 9.0 W/kg at 837 MHz continuous-wave (CW) microwave irradiation for 2 h. The TP53 protein levels were measured by Western blot at 2, 8, 24 and 48 h after treatment. The TP53 protein levels in microwave-treated cells, sham-treated cells, and untreated cells remained unchanged relative to each other at all times tested (Fisher test and Student-Newman-Keuls test, P > 0.05). No morphological alterations were observed in microwave-treated cells compared to sham-treated cells. We conclude that TP53 protein expression levels in cultured human fibroblast cells do not change significantly during a 48-h period after exposure to 837 MHz continuous microwaves for 2 h at SAR levels of 0.9 or 9.0 W/kg.

Whole body exposure of rats to microwaves emitted from a cell phone does not affect the testes

“…Because the use of mobile phones operating in the 900 MHz frequency band is widespread and increasing rapidly, the question of whether MW of these frequencies could cause reproductive or developmental effects is of public interest. In recent years, results of increasing number of studies related to effects of microwave radiation from cellular phones and base stations have shown that the MWemitted from cellular phones are not dangerous [Fritze et al, 1997;Jensh, 1997;Malyapa et al, 1997;Röschke and Mann, 1997;Stagg et al, 1997;de Seze et al, 1998;Li et al, 1999;Bornhausen and Scheingraber, 2000;Gos et al, 2000;Grant and Schlegel, 2000;Sienkiewicz et al, 2000;Tsurita et al, 2000;Maes et al, 2001]. However, because some of the studies related to MWemitted from mobile phones have reported positive findings [Freude et al, 1998;Dasdag et al, 1999;Dasdag et al, 2000;Schirmacher et al, 2000], additional research is required.…”

“…It also has been shown that p53 can respond to nongenotoxic stressors, such as heat shock, ribonucleotide depletion, and treatment with high levels of calcium phosphate. Upon exposure to cellular stress, the level of p53 tumor suppressor protein increases 2-10 fold within 1-24 h, activating transcription of a variety of target genes [Li et al, 1999]. Li et al [1999] reported that p53 tumor suppressor protein in normal human fibroblasts does not respond to 837 MHz microwave exposure.…”

The Effects of 2100 mhz Radio Frequency Radiation on Thyroid Tissues

“…The electromagnetic radiation emitted has an effect especially on the user. Although many studies have revealed that microwave radiation exposure has no detrimental effect on human health whatsoever (1)(2)(3)(4)(5)(6), some studies have shown the contrary (7)(8)(9). The study aims to reveal the effect of 2100 MHz radio frequency radiation on thyroid tissues of the rats in early and late stages.…”

Effects of mobile phone radiofrequency on the structure and function of the normal human hemoglobin

“…Bismuto et al [1] showed that Measurements of absorption spectroscopy, circular dichroism and fluorescence emission decay in the frequency domain do not exhibit any influence of the microwave radiation on the native structural state of myoglobin. Li et al [49] reported that 837 MHz microwave exposure of TP53 tumor suppressor protein in normal human fibroblasts do not change significantly.…”