TPX2 Is a Prognostic Marker and Contributes to Growth and Metastasis of Human Hepatocellular Carcinoma

Huang, Yuqi; Guo, Wenbin; Kan, Heping

doi:10.3390/ijms151018148

Cited by 51 publications

(56 citation statements)

References 27 publications

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“…TPX2 expression detected by immunohistochemical analysis was associated with depth of tumor, lymph node metastasis, and remote metastasis in colon cancer [13]. TPX2 expression analyzed by immunohistochemistry was associated with poor patient survival in many types of cancer [11–14]. TPX2 is an independent prognostic indicator for poor patient survival in hepatic cancer [14].…”

Section: Discussionmentioning

confidence: 99%

“…TPX2 expression analyzed by immunohistochemistry was associated with poor patient survival in many types of cancer [11–14]. TPX2 is an independent prognostic indicator for poor patient survival in hepatic cancer [14]. In colon cancer, TPX2 is strongly associated with the progression of colorectal adenoma to carcinoma [16].…”

Section: Discussionmentioning

confidence: 99%

“…For instance, TPX2 -siRNA decreased the viability and proliferation capacity of colon, cervical, and hepatic cancer cell lines [13, 14, 18]. Similarly, TPX2 -siRNA induced cell apoptosis in hepatic cancer cell lines [12].…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, TPX2 expression is a marker of worse tumor prognosis in several cancers [11–14]. These observations suggest that TPX2 plays a role in the oncogenesis of at least some malignancies.…”

Section: Introductionmentioning

confidence: 99%

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TPX2 expression is associated with poor survival in gastric cancer

et al. 2017

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BackgroundTargeting protein for Xenopus kinesin-like protein 2 (TPX2) is a microtubule-associated protein required for microtubule formation in human cells. Several studies have demonstrated that TPX2 is overexpressed in multiple tumor types and promotes tumor growth and metastasis. However, there have been few reports regarding its role in gastric cancer. In this study, we evaluated TPX2 expression and investigated its correlations with gastric cancer clinicopathological features and prognosis.MethodsTumor samples were obtained from 290 patients with gastric adenocarcinoma who had undergone gastrectomy. The expression of TPX2 protein was examined using immunohistochemical staining. TPX2 messenger RNA (mRNA) levels were evaluated using real-time quantitative reverse transcription PCR in 19 of the gastric cancer tumors and adjacent normal tissues.ResultsThe mRNA levels of TPX2 were significantly higher in gastric cancer tissues than in matched adjacent normal tissues (p = 0.004). In the immunohistochemical analysis, TPX2 overexpression was found in 123 (42.4%) of 290 patients. High TPX2 expression was positively associated with age, type of histology, depth of tumor, lymph node metastasis, stage, and remote metastasis or recurrence. High TPX2 expression was significantly associated with poorer disease-specific survival (p = 0.004) and relapse-free interval (p = 0.013).ConclusionsOur results indicated that high TPX2 expression was associated with tumor progression and poor survival in gastric cancer.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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TPX2 expression is associated with poor survival in gastric cancer

et al. 2017

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“…In vitro, TPX2 knockdown significantly inhibited cell proliferation and viability in Hep3B and HepG2 cells. Moreover, TPX2 knockdown noticeably slowed down tumor growth in a nude mouse xenograft model and prominently suppressed HCC cell invasion and migration [6]. TPX2 localizes to the nucleus during S-phase and G2 and at the mitotic spindle poles during mitosis [7] emphasizing its likely involvement in cell division.…”

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confidence: 99%

Targeting HCC Therapy: On or Off ToPiX?

Andl

2015

Dig Dis Sci

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TPX2‐p53‐GLIPR1 regulatory circuitry in cell proliferation, invasion, and tumor growth of bladder cancer

Liang

Yang

et al. 2017

J of Cellular Biochemistry

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The targeting protein for Xenopus kinesin-like protein 2 (TPX2) is associated with the metastasis and prognosis of bladder cancer. p53 is closely related to the progression of bladder cancer. Human glioma pathogenesis-related protein 1 (GLIPR1) is a p53 target gene with antitumor activity. This study aims to explore the interplay between TPX2, p53, and GLIPR1 and its correlation with cell proliferation, invasion, and tumor growth in bladder cancer. Here, Western blot and qRT-PCR analysis revealed that TPX2 at both mRNA and protein levels was up-regulated in bladder carcinoma tissues compared to their paired adjacent normal tissues. Additionally, tissues expressing high TPX2 level exhibited high p53 level and low GLIPR1 level. The expressions of TPX2 and p53 in non-muscle-invasive bladder cancer cells (KK47 and RT4) were lower than those in muscle-invasive bladder cancer cells (T24, 5637, and UM-UC-3), while GLIPR1 showed the converse expression pattern. Further investigation revealed that TPX2 activated the synthesis of p53; and GLIPR1 is up-regulated by wild-type (wt)-p53 but not affected by mutated p53; Additionally, GLIPR1 inhibited TPX2. These data suggested a TPX2-p53-GLIPR1 regulatory circuitry. Meanwhile, TPX2 overexpression promoted while overexpression of GLIPR1 or p53 inhibited bladder cancer growth. Interestingly, in T24 cells with mutated p53, p53 silence suppressed bladder cancer growth. This study identified a novel TPX2-p53-GLIPR1 regulatory circuitry which modulated cell proliferation, migration, invasion, and tumorigenicity of bladder cancer. Our findings provide new insight into underlying mechanisms of tumorigenesis and novel therapeutic options in bladder cancer.

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TPX2 Is a Prognostic Marker and Contributes to Growth and Metastasis of Human Hepatocellular Carcinoma

Cited by 51 publications

References 27 publications

TPX2 expression is associated with poor survival in gastric cancer

TPX2 expression is associated with poor survival in gastric cancer

Targeting HCC Therapy: On or Off ToPiX?

TPX2‐p53‐GLIPR1 regulatory circuitry in cell proliferation, invasion, and tumor growth of bladder cancer

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