Trace determination of sulfide by reversed-phase ion-interaction chromatography using pre-column derivatization

Haddad, Paul R.; Heckenberg, A.L.

doi:10.1016/0021-9673(88)90054-4

Cited by 16 publications

(6 citation statements)

References 5 publications

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“…The amount of H 2 S in the medium was determined by reversed-phase high-performance liquid chromatography (HPLC) as MB (12,40). To assay the stability of NaHS, NaHS (300 µM) was added to 10 cm culture dish containing 10 ml of DMEM supplemented with 10% FBS, penicillin (100 U/ml) and streptomycin (100 µg/ml).…”

Section: Measurement Of H 2 Smentioning

confidence: 99%

Hydrogen Sulfide Protects HT22 Neuronal Cells from Oxidative Stress

Kimura

Dargusch

Schubert

et al. 2006

Antioxidants & Redox Signaling

274

174

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Hydrogen sulfide (H2S) is a neuromodulator in the brain and a relaxant for smooth muscle. H2S protects primary cortical neurons from oxidative stress by increasing the intracellular concentrations of glutathione, the major antioxidant in cells. However, changes in glutathione alone are not sufficient to account for full protection in all types of nerve cells. H2S is here shown to protect an immortalized mouse hippocampal cell line from oxidative glutamate toxicity by activating ATP-dependent K+ (KATP) and Cl- channels, in addition to increasing the levels of glutathione. The present study therefore identifies a novel pathway for H2S protection from oxidative stress.

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Section: Measurement Of H 2 Smentioning

confidence: 99%

Hydrogen Sulfide Protects HT22 Neuronal Cells from Oxidative Stress

Kimura

Dargusch

Schubert

et al. 2006

Antioxidants & Redox Signaling

274

174

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“…Chromatographic techniques have been applied to the detection of sulfide in a number of complex matrices, including environmental and effluent water [3][4][5], pharmaceutical preparations [6], soil samples [7], body fluids [8] and human blood [9], mainly due to the separation capabilities of those techniques [10,11]. Reported detection systems include conductivity [6] and flame photometry [4,7,12], as well as fluorimetry and spectrophotometry [6,13,14]. While most techniques can detect sulfide directly, there have been a number of precolumn derivatization procedures based on methylene blue [15], thionine [14] and fluorescent label monobromobimane [8].…”

Section: Introductionmentioning

confidence: 99%

“…Reported detection systems include conductivity [6] and flame photometry [4,7,12], as well as fluorimetry and spectrophotometry [6,13,14]. While most techniques can detect sulfide directly, there have been a number of precolumn derivatization procedures based on methylene blue [15], thionine [14] and fluorescent label monobromobimane [8]. In particular, one of the best known high performance liquid chromatography (HPLC) methods for the determination of sulfide involves the application of the methylene blue reaction [14,16].…”

Section: Introductionmentioning

confidence: 99%

“…While most techniques can detect sulfide directly, there have been a number of precolumn derivatization procedures based on methylene blue [15], thionine [14] and fluorescent label monobromobimane [8]. In particular, one of the best known high performance liquid chromatography (HPLC) methods for the determination of sulfide involves the application of the methylene blue reaction [14,16]. The reaction of sulfide with N,N-dimethyl-p-phenylenediamine leads to the conversion of the anion to a methylene blue derivative, which is determined by reversed-phase HPLC and spectrophotometric detection.…”

Section: Introductionmentioning

confidence: 99%

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A derivatization approach using pyrylium salts for the sensitive and simple determination of sulfide in spring water by high performance liquid chromatography

Rembisz¹,

Bzdurska²,

Obiedzińska³

et al. 2015

Journal of Chromatography A

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“…7,8 Methods based on GC-FPD 9 and IC-ECD 10 are sensitive, but the former requires tedious purification steps, while the latter suffers from the problem of adsorption of sulfide onto the ion-exchange column. The HPLC method, based on the derivatization of sulfide to methylene blue, 11 has the advantage of eliminating problems associated with the batch spectrophotometric methods such as the turbidity of the sample and the formation of coloured byproducts. However, its drawbacks are the insufficient sensitivity and reproducibility, and liquid chromatography on the C 18 column gives broad and tailing peaks.…”

Section: Introductionmentioning

confidence: 99%

Solid-phase extraction and spectrophotometric determination of hydrogen sulfide in air and water utilizing ethylene blue formation

Singh

Gosain

Mishra

et al. 2000

Analyst

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Hydrogen sulfide in water stabilized with or absorbed from air in an alkaline mixture of zinc acetate and EDTA was treated with 4-N,N-diethylaminoaniline and iron(III) in sulfuric acid medium and the absorbance of ethylene blue was measured at 670 nm after 5 min. The molar absorptivity was found to be 9.42 3 10 5 l mol 21 cm 21 . The ethylene blue dye could be preconcentrated by solid-phase extraction on a CN sorbent and eluted by potassium bromide solution in water-methanol leading to an enrichment factor of at least 50. Coloured samples were subjected to clean-up by using Lichrolut EN and the colourless eluate was treated for colour development. Rectilinear calibration graphs were obtained over the range 10-800 mg l 21 sulfide (r = 0.9997) without solid-phase extraction, and 1-100 mg l 21 sulfide (r = 0.9989) after solid-phase extraction of the dye. The limits of detection were 5 mg l 21 and 0.2 mg l 21 sulfide without and after solid-phase extraction, respectively. The interference of up to 100 mg l 21 thiols, 50 mg l 21 sulfite and 10 mg l 21 thiosulfate could be avoided by their masking with N-ethylmaleimide. Up to 10 mg l 21 fluoride and 100 mg l 21 thiocyanate did not cause any interference. Interference of up to 100 mg l 21 fluoride was avoided by masking with zirconyl nitrate. The method has been validated by analysing spiked river water samples, when the average recovery was 99% (range 96-104%) with an RSD of 3% (range 2-4%). Sulfide has been detected in many river waters at low mg l 21 levels. Experimental EquipmentAll spectrophotometric measurements were made with an ATI-Unicam UV2-100 UV/visible spectrophotometer (Cambridge, UK) using 1 cm matched quartz cells.Solid-phase extraction (SPE) cartridges, 2.8 ml, packed with CN sorbent (500 mg) (Alltech, Deerfield, IL, USA) were used.

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Trace determination of sulfide by reversed-phase ion-interaction chromatography using pre-column derivatization

Cited by 16 publications

References 5 publications

Hydrogen Sulfide Protects HT22 Neuronal Cells from Oxidative Stress

Hydrogen Sulfide Protects HT22 Neuronal Cells from Oxidative Stress

A derivatization approach using pyrylium salts for the sensitive and simple determination of sulfide in spring water by high performance liquid chromatography

Solid-phase extraction and spectrophotometric determination of hydrogen sulfide in air and water utilizing ethylene blue formation

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