Trace determination of urinary 3-hydroxybenzo[a]pyrene by automated column-switching high-performance liquid chromatography

A toxicokinetic study to elucidate 3‐hydroxybenzo(a)pyrene atypical urinary excretion profile following intravenous injection of benzo(a)pyrene in rats

“…Urine samples were analyzed using an analytical procedure adapted from Simon et al (2000). After the urine samples had thawed, they were heated for 30 min at 37 °C to dissolve possible precipitate, and well homogenized.…”

“…Therefore, 3-hydroxybenzo(a)pyrene (3-OHBaP) has been explored as a biomarker of particular interest since it is the metabolite of BaP, a carcinogenic PAH and one of its major metabolites measured in urine (Bouchard and Viau, 1996;Wang et al, 2003). This is a non-negligible aspect considering that BaP metabolites are present in human urine in very small amounts, 1000-10 000-fold less than 1-OHP (Gendre et al, 2002), and their urinary detection in humans requires highly sensitive analytical procedures (Barbeau et al, 2008;Simon et al, 2000).…”

Development of a gas chromatography/mass spectrometry method to quantify several urinary monohydroxy metabolites of polycyclic aromatic hydrocarbons in occupationally exposed subjects

“…To optimize the derivatization three different silylating agents, producing the same derivative, were compared: BSTFA, Trisil, and Trisil Z. For each reagent, two different temperatures (45 • C and 70 • C) and four different incubation times (15,30, 60, 90 min), for a total of 24 experimental conditions, were tested. The two reaction temperatures, 45 • C and 70 • C, were chosen as the optimum temperature suggested respectively for BSTFA and Trisil Z by the suppliers, while no specific temperature was suggested for Trisil.…”

Determination of 3-OHB[a]P and (+)-anti-BPDE in Rats Blood and Brain Tissue of B[a]P Exposure by HPLC with Fluorescence Detection

“…Among the B[a]P hydroxylated metabolites, 3-hydroxybenzo(a)pyrene (3-OHB[a]P) has been one of the most studied biomarkers with respect to PAH exposure [22][23][24][25][26]. A sensitive high-performance liquid chromatography (HPLC) method for determining 3-OHB[a]P in urine has been developed that uses a column HPLC system coupled with fluorescence detection (FD) [27,28].…”