The arsenic contamination accident in Yangzonghai Lake, Yunnan has been of wide concern. In order to investigate the arsenic distribution and concentration trends after the accident, samples including lake water, sediments, soil, aquatic organisms and crops were collected in November 2008, as well as in February, May and September 2009. The average arsenic concentrations (arithmetic average) in lake water in the four sampling events were 176.9, 147.3, 159.3, and 161.1 g/L, while those in the sediments were 32.87, 62.41, 62.99, and 46.96 g/g, respectively. The highest content of total arsenic in soil in the vicinity of Yangzonghai was 23.33 µg/g, which was below the limits of the relevant national standard. The total arsenic levels in most aquatic plants were in the range of 100-200 g/g, with Vallisneria natans (Lour.) Hara having the highest concentration of ~300 g/g. The arsenic levels of fish and shrimps were in the range of 1.52-11.4 g/g (dry weight).