Trace quantification of 1-octacosanol and 1-triacontanol and their main metabolites in plasma by liquid–liquid extraction coupled with gas chromatography–mass spectrometry

Haim, Daniela; Berríos, Miriam; Valenzuela, Alfonso; Videla, Luis A.

doi:10.1016/j.jchromb.2009.10.034

Cited by 16 publications

(12 citation statements)

References 11 publications

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“…Results were in good agreement with tag content determined by classical GC method [5]. Compared with published GC-FID or GC-MS method [4][5][6], the LOD of published GC-FID or GC-MS method ranged between 0.003 and 0.05 mg/L, and the LOD of developed UPLC-ELSD method is 1.0 mg/L. UPLC-ELSD was found to give lower sensitivity, but is sensitive enough for determination of 1-octacosanol content in raw material and health products.…”

Section: Analysis Of Real Samples and Comparison With The Classical Msupporting

confidence: 80%

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UPLC-ELSD Determination of 1-Octacosanol in Raw Material and Health Products

et al. 2011

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Determination of the levels of 1-octacosanol is important in food stuff for the study of its pharmacological activities and health benefits. In this study, a novel, simple and fast internal standard method for the non-derivatization ultra-performance liquid chromatographic determination of 1-octacosanol in raw materials and health products was developed and validated based on evaporative light scattering detection. The linearity (r 2 [ 0.998), recovery (99.1-100.2%, RSD \2.7%), intra-and inter-day precision (RSD \3.8%), limit of detection (1.0 mg/L), limit of quantification (2.2 mg/L) of the 1-octacosanol were determined. The method was successfully applied to nine real 1-octacosanol products. The results of analyses had close agreement with the labeled claims of 1-octacosanol content in these products. Compared with the classical gas chromatography method, the developed method was simpler, faster and more environmentally friendly due to avoiding any derivatization step. This protocol represents a rapid and feasible method for quality control of 1-octacosanol products.

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Section: Analysis Of Real Samples and Comparison With The Classical Msupporting

confidence: 80%

“…Published methods for the determination of 1-octacosanol are mainly based on GC-FID or GC-MS [4][5][6]. Due to difficulty with vaporization, a derivatization process is usually needed before GC analysis, making the determinations less certain than when this process is omitted.…”

Section: Introductionmentioning

confidence: 99%

UPLC-ELSD Determination of 1-Octacosanol in Raw Material and Health Products

et al. 2011

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“…The composite coating was prepared by immersing substrates in 1-triacontanol and OTS in ethanol. 1-Triacontanol has been previously used in biological and organic chemistry, [25][26][27][28][29][30] but not to prepare superhydrophobic and superoleophilic materials. The coating solution could be reused after aged for 10 days, which is a significantly improvement in the practical application relative to our previous research using liquid OTS as coating material.…”

Section: Introductionmentioning

confidence: 99%

Highly efficient oil/water separation and excellent self-cleaning surfaces based on 1-triacontanol-polymerized octadecylsiloxane coatings

Jin

Jiang

et al. 2015

Applied Surface Science

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“…Finally 0.5 µL were injected automatically into the gas chromatography (model 7890A, Agilent Technologies, California, USA) coupled with an inert mass spectrometry detector (model 5975C, Agilent Technologies, California, USA) for quantitative analysis. The chromatographic conditions were described by Haim et al (2009).…”

Section: Bioavailability Of Policosanol and Longchain Fatty Acidsmentioning

confidence: 99%

“…Therefore, the technique allowed for the measuring of esterified and non-esterified analytes present in the plasma. The detailed methodology and validation of the technique were reported by our group (Haim et al, 2009). …”

Section: Determination Of Plasmatic Total Policosanol and Long-chain mentioning

confidence: 99%

The oleic acid esterification of policosanol increases its bioavailability and hypocholesterolemic action in rats

Haim¹,

Valenzuela²,

Brañes³

et al. 2012

Grasas y Aceites

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(DH, and AV); d Naturalis S.A., Santiago, Chile (MCB, and MF) *Corresponding author: dnhaim@miuandes.cl RESUMEN La esterificación de ácido oleico en policosanoles aumenta su biodisponibilidad y el efecto hipocolesterolémico en ratasLos Policosanoles están formados por una mezcla de alcoholes alifáticos de cadena larga y se obtienen de las ceras de la caña de azúcar. Más de cincuenta estudios indican que los policosanoles reducen el colesterol sérico, mientras que otros no logran reproducir este efecto. El objetivo de esta investigación fue evaluar la biodisponibilidad de policosanoles esterificados y no esterificados (NEP), en relación con sus efectos hipocolesterolémicos. Para ello, a ratas Sprague Dawley se les administró una dosis oral diaria de 100 mg kg -1 de NEP, 117 mg kg -1 de policosanoles esterificados con ácido butírico (BAEP), ó 164 mg kg -1 de policosanoles esterificados con ácido oleico (OAEP). La absorción de los policosanoles se evaluó en el plasma entre 0 y 3 horas después de la ingestión. Para evaluar los cambios en el colesterol total, colesterol-LDL, colesterol-HDL y triglicéridos en el plasma y en la fosforilación de la hígado 3-hidroxi-3-metilglutaril coenzima A reductasa (HMG-CoA red), la ingesta de las ratas fue suplementada con policosanoles no esterificados o esterificados durante 5 semanas. Los resultados indicaron que la absorción de los policosanoles fue significativamente mayor en las ratas tratadas con los OAEP que en las sometidas a los NEP o las que se les administró BAEP. Los OAEP redujeron significativamente el colesterol plasmático total y el colesterol-LDL de las ratas, además de aumentar 5.6 veces (P < 0,05) sobre los valores control, en el contenido hepático de la HMG-CoA fosforilada red. En conclusión, la esterificación de policosanoles con ácido oleico aumenta la biodisponibilidad de los policosanoles, y mejora significativamente el perfil de lípidos séricos en ratas normocolesterolémicos en asociación con la inactivación de la colesterogénesis control HMG-CoA red. PALABRAS-CLAVE: Absorción de Policosanoles -Biodisponibilidad -Efecto reductor de colesterol -Octacosanol -Policosanol -Triacontanol -Ratas. SUMMARY The oleic acid esterification of policosanol increases its bioavailability and hypocholesterolemic action in ratsPolicosanol comprises a mixture of long-chain aliphatic alcohols from sugarcane wax. More than 50 studies indicate that policosanol decreases serum cholesterol, while others failed to reproduce this effect. The objective of this investigation was to assess the bioavailability of esterified policosanol and non-esterified policosanol (NEP), in relation to their hypocholesterolemic effects. Sprague Dawley rats were given a daily oral dose of 100 mg/kg of NEP, 117 mg kg -1 of butyric acid esterified policosanol (BAEP), or 164 mg kg -1 of oleic acid esterified policosanol (OAEP). Policosanol absorption was evaluated in plasma between 0 and 3 hours after ingestion. To assess changes in total cholesterol, LDL-cholesterol, HDLcholesterol and triacylglycerols in ...

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Trace quantification of 1-octacosanol and 1-triacontanol and their main metabolites in plasma by liquid–liquid extraction coupled with gas chromatography–mass spectrometry

Cited by 16 publications

References 11 publications

UPLC-ELSD Determination of 1-Octacosanol in Raw Material and Health Products

UPLC-ELSD Determination of 1-Octacosanol in Raw Material and Health Products

Highly efficient oil/water separation and excellent self-cleaning surfaces based on 1-triacontanol-polymerized octadecylsiloxane coatings

The oleic acid esterification of policosanol increases its bioavailability and hypocholesterolemic action in rats

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