Traces of isotopic reactive species produced from a non-thermal plasma jet in bio-molecules

Abstract

Heavy water (D 2 O) is introduced into a non-thermal plasma jet (NTPJ) device to generate deuterium monoxide (OD) radicals instead of hydroxyl (OH) radicals. An NTPJ generated from a vapor mixture of N 2 /H 2 O and N 2 /D 2 O is applied to a cell membrane component and its effects are analyzed by means of 1 H NMR, GC-FID and TOF-SIMS spectroscopies. The results show that OH and OD radical species induce similar levels of oxidative breakage of lipid molecules. In addition, the 2 H NMR spectra show that deuteriums are incorporated into the lipid oxidative products. In order to trace these effects in vivo, E. coli bacteria are treated with an NTPJ and analyzed using NanoSIMS. Deuterium is observed in both the cytoplasm and membrane, which are colocalized well with nitrogen and phosphorus atoms. The high colocalization of D atoms inside E. coli provides the first direct and visual evidence of the role of OD radicals, which may be utilized to visualize OH radical interactions inside cells.

Cold Plasma‐Mediated Treatments for Shelf Life Extension of Fresh Produce: A Review of Recent Research Developments

“…In addition, optical emission spectroscopy, optical absorption spectroscopy, isotopic tracer, electron spin resonance (ESR) spectroscopy, and mass spectrometry are all common methods for analyzing the reactive species induced by the plasma in gas–liquid interface and beneath the liquid (Lee, Kwak et al., ; Zhang et al., ). For example, ESR spectroscopy was used by Wu et al.…”

Surface and Structure of Phosphatidylcholine Membranes Reconstructed with CoFe2O4 Nanoparticles

“…We have analyzed lipid suspension in deuterated water D 2 O (red line 1 in Figure ) and the same solution containing NPs at a concentration of 60 μL/mL (black line 2 in Figure ). NMR lines of the aliphatic groups corresponding to lipid hydrophobic tails are found in the 0–2 ppm range . The fatty acids give lines in the range of larger chemical shifts (3–6 ppm).…”

Quasi-2D Halide Perovskite Memory Device Formed by Acid–Base Binary Ligand Solution Composed of Oleylamine and Oleic Acid

“…Additionally, OA (OlAm) has a double bond between carbons 9 (26) and 10 ( 27), each of which are coupled to a single olefinic hydrogen with a signal at ∼5.45 ppm, which is well separated from the other signals. 37 The hydrogen neighboring the double bond can also be observed in the resonance peak at 2.07 ppm (8, 11, 25, and 28). Additional resonance peaks from 0.90 to 1.84 ppm correspond to hydrogen atoms associated with the methyl group, α-hydrogens, and β-hydrogens.…”

“…In particular, NMR values of 2.60 ppm (1) and 2.42 ppm (19) are assigned to the α-CH 2 resonances of OA and OlAm in the 1 H NMR spectra, respectively. Additionally, OA (OlAm) has a double bond between carbons 9 (26) and 10 (27), each of which are coupled to a single olefinic hydrogen with a signal at ∼5.45 ppm, which is well separated from the other signals . The hydrogen neighboring the double bond can also be observed in the resonance peak at 2.07 ppm (8, 11, 25, and 28).…”