Tracking and Quantification of Magnetically Labeled Stem Cells Using Magnetic Resonance Imaging

Goodfellow, Forrest T.; Simchick, Gregory; Mortensen, Luke J.; Stice, Steven L.; Zhao, Qun

doi:10.1002/adfm.201504444

Cited by 37 publications

(29 citation statements)

References 177 publications

(188 reference statements)

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“…The neurite outgrowth assay was conducted as previously described [ 32 ]. Briefly, 28 DIV neurons were seeded onto Costar ® 96-well cell culture plates at a density of 15,000 cells per well.…”

Section: Methodsmentioning

confidence: 99%

Strain-Dependent Consequences of Zika Virus Infection and Differential Impact on Neural Development

Goodfellow

Willard

Wang

et al. 2018

Viruses

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Maternal infection with Zika virus (ZIKV) during pregnancy can result in neonatal abnormalities, including neurological dysfunction and microcephaly. Experimental models of congenital Zika syndrome identified neural progenitor cells as a target of viral infection. Neural progenitor cells are responsible for populating the developing central nervous system with neurons and glia. Neural progenitor dysfunction can lead to severe birth defects, namely, lissencephaly, microcephaly, and cognitive deficits. For this study, the consequences of ZIKV infection in human pluripotent stem cell-derived neural progenitor (hNP) cells and neurons were evaluated. ZIKV isolates from Asian and African lineages displayed lineage-specific replication kinetics, cytopathic effects, and impacts on hNP function and neuronal differentiation. The currently circulating ZIKV isolates exhibit a unique profile of virulence, cytopathic effect, and impaired cellular functions that likely contribute to the pathological mechanism of congenital Zika syndrome. The authors found that infection with Asian-lineage ZIKV isolates impaired the proliferation and migration of hNP cells, and neuron maturation. In contrast, the African-lineage infections resulted in abrupt and extensive cell death. This work furthers the understanding of ZIKV-induced brain pathology.

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Section: Methodsmentioning

confidence: 99%

Strain-Dependent Consequences of Zika Virus Infection and Differential Impact on Neural Development

Goodfellow

Willard

Wang

et al. 2018

Viruses

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“…Implantation of SPIO-tagged MSCs in experimental models lead to high resolution MRI, supporting the beneficial behavior of SPIO labeling for stable MRI tracing [80]. Modification of SPIOs and their addition to human neural stem cells (hNSCs) allowed tracking of implanted NSCs by MRI for up to three months, without any significant impairment in cell viability or proliferation [81] (Table 1). Currently, developing techniques such as magnetic resonance imaging (MRI), computed tomography (CT), positron emission tomography (PET), and near-infrared (NIR) fluorophores offer noninvasive models for tracking transplanted stem cells.…”

Section: Nanotechnology Application For Monitoring Stem-cell Therapy mentioning

confidence: 84%

Application of Nanotechnology in Stem-Cell-Based Therapy of Neurodegenerative Diseases

et al. 2020

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In addition to adverse health outcomes, neurological disorders have serious societal and economic impacts on patients, their family and society as a whole. There is no definite treatment for these disorders, and current available drugs only slow down the progression of the disease. In recent years, application of stem cells has been widely advanced due to their potential of self-renewal and differentiation to different cell types which make them suitable candidates for cell therapy. In particular, this approach offers great opportunities for the treatment of neurodegenerative disorders. However, some major issues related to stem-cell therapy, including their tumorigenicity, viability, safety, metastases, uncontrolled differentiation and possible immune response have limited their application in clinical scales. To address these challenges, a combination of stem-cell therapy with nanotechnology can be a solution. Nanotechnology has the potential of improvement of stem-cell therapy by providing ideal substrates for large scale proliferation of stem cells. Application of nanomaterial in stem-cell culture will be also beneficial to modulation of stem-cell differentiation using nanomedicines. Nanodelivery of functional compounds can enhance the efficiency of neuron therapy by stem cells and development of nanobased techniques for real-time, accurate and long-lasting imaging of stem-cell cycle processes. However, these novel techniques need to be investigated to optimize their efficiency in treatment of neurologic diseases.

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“…MRI-based SC imaging provides high spatial resolution and reasonable imaging time (minutes) to noninvasively track labeled transplanted SCs [52,53]. MRI has been frequently used to monitor morphological and SC migration after engraftment [14,17].…”

Section: Mrimentioning

confidence: 99%

Accomplishments and challenges in stem cell imaging in vivo

Bose

Mattrey

2019

Drug Discovery Today

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Tracking and Quantification of Magnetically Labeled Stem Cells Using Magnetic Resonance Imaging

Cited by 37 publications

References 177 publications

Strain-Dependent Consequences of Zika Virus Infection and Differential Impact on Neural Development

Strain-Dependent Consequences of Zika Virus Infection and Differential Impact on Neural Development

Application of Nanotechnology in Stem-Cell-Based Therapy of Neurodegenerative Diseases

Accomplishments and challenges in stem cell imaging in vivo

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