Tracking Dynamic Microvascular Changes during Healing after Complete Biopsy Punch on the Mouse Pinna Using Optical Microangiography

Abstract

Optical microangiography (OMAG) and Doppler optical microangiography (DOMAG) are two non-invasive techniques capable of determining the tissue microstructural content, microvasculature angiography, and blood flow velocity and direction. These techniques were used to visualize the acute and chronic microvascular and tissue responses upon an injury in vivo. A tissue wound was induced using a 0.5 mm biopsy punch on a mouse pinna. The changes in the microangiography, blood flow velocity and direction were quantified for the acute (<30 min) wound response and the changes in the tissue structure and microangiography were determined for the chronic wound response (30 min–60 days). The initial wound triggered recruitment of peripheral capillaries, as well as redirection of main arterial and venous blood flow within 3 min. The complex vascular networks and new vessel formation were quantified during the chronic response using fractal dimension. The highest rate of wound closure occurred between days 8 and 22. The vessel tortuosity increased during this time suggesting angiogenesis. Taken together, these data signify that OMAG has the capability to track acute and chronic changes in blood flow, microangiography and structure during wound healing. The use of OMAG has great potential to improve our understanding of vascular and tissue responses to injury in order to develop more effective therapeutics.

Motion artifact and background noise suppression on optical microangiography frames using a naïve Bayes mask

“…Optical microangiography (OMAG) is a technique for processing OCT data, which allows the extraction of the three-dimensional microvasculature from the OCT images, with capillary resolution [2]. OMAG has been used in a variety of applications which include tracking wounds [3] and burns [4] in mouse ear, imaging of the human corneo-scleral limbus [5] and human retina [6], studies of mouse brain [7], and others.…”

Label-Free Optical Imaging of Lymphatic Vessels Within Tissue Beds IN VIVO

“…Perhaps this enlargement was due to the inflammatory and immune response at the injury site. The biopsy punch model was very similar to the one previously reported by Jung et al [72] where they created a biopsy punch and complete removal of tissue on the ear skin flap and monitored the vascular remodeling and healing process along the time. In this paper, we also monitor the response of lymphatic vessels along with blood vessels to that injury model.…”

External Compression Versus Intravascular Injection: A Mechanistic Animal Model of Filler-Induced Tissue Ischemia

“…[20][21][22] Optical microangiography uses the inherent properties of optical coherence tomography signals to separate moving particles from static scattering elements within tissues. This technique allows precise localization of blood perfusion in 3D microstructures.…”

“…Detailed image acquisition protocols have been previously published. [20][21][22] To image a larger field of view (2 × 2 cm), a montage of adjacent individual pictures must be created. 21 Optical microangiography affords us a label-free in vivo view of filler-induced malperfusion at the single capillary level within tissue beds.…”