Tracking GLUT2 Translocation by Live-Cell Imaging

Tsytkin-Kirschenzweig, Sabina; Cohen, Merav; Nahmias, Yaakov

doi:10.1007/978-1-4939-7507-5_18

Cited by 5 publications

(2 citation statements)

References 29 publications

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“…In livers of control animals, Pparα expression was higher at ZT14 in comparison to ZT20, a pattern that was still evident in tumor-bearing mice ( Figure 5 D). We also evaluated the expression of Glut2 , a gene that encodes a glucose transporter [ 96 , 97 ]. Glut2 transcripts did not show an oscillatory pattern in control mice; however, in tumor-bearing mice, Glut2 expression was higher at ZT14 in comparison to ZTs 8 and 20 ( Figure 5 E).…”

Section: Resultsmentioning

confidence: 99%

Non-Metastatic Cutaneous Melanoma Induces Chronodisruption in Central and Peripheral Circadian Clocks

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Moraes

Magalhães-Marques

et al. 2018

IJMS

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The biological clock has received increasing interest due to its key role in regulating body homeostasis in a time-dependent manner. Cancer development and progression has been linked to a disrupted molecular clock; however, in melanoma, the role of the biological clock is largely unknown. We investigated the effects of the tumor on its micro- (TME) and macro-environments (TMaE) in a non-metastatic melanoma model. C57BL/6J mice were inoculated with murine B16-F10 melanoma cells and 2 weeks later the animals were euthanized every 6 h during 24 h. The presence of a localized tumor significantly impaired the biological clock of tumor-adjacent skin and affected the oscillatory expression of genes involved in light- and thermo-reception, proliferation, melanogenesis, and DNA repair. The expression of tumor molecular clock was significantly reduced compared to healthy skin but still displayed an oscillatory profile. We were able to cluster the affected genes using a human database and distinguish between primary melanoma and healthy skin. The molecular clocks of lungs and liver (common sites of metastasis), and the suprachiasmatic nucleus (SCN) were significantly affected by tumor presence, leading to chronodisruption in each organ. Taken altogether, the presence of non-metastatic melanoma significantly impairs the organism’s biological clocks. We suggest that the clock alterations found in TME and TMaE could impact development, progression, and metastasis of melanoma; thus, making the molecular clock an interesting pharmacological target.

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Section: Resultsmentioning

confidence: 99%

Non-Metastatic Cutaneous Melanoma Induces Chronodisruption in Central and Peripheral Circadian Clocks

Assis

Moraes

Magalhães-Marques

et al. 2018

IJMS

View full text Add to dashboard Cite

show abstract

“…Recent evidence strongly suggests that the intracellular localization of GLUT1 and GLUT2 may change in response to the energy requirements of the cell [ 38 , 39 ]. Consistent with this evidence, we evaluated whether hypoglycaemia in tanycytes induces the mobilization of GLUT1, GLUT2, and GLUT6 to the ER-subcellular structures to promote glucose export from the ER to the extracellular environment.…”

Section: Resultsmentioning

confidence: 99%