Tracking HOCl by an incredibly simple fluorescent probe with AIE plus ESIPT in vitro and in vivo

Qu, Wangbo; Guo, Taiyu; Yang, Bin; Tian, Ruowei; Qiu, Shuang; Chen, Xinyue; Geng, Zhirong; Wang, Zhilin

doi:10.1016/j.saa.2022.121649

Cited by 9 publications

(4 citation statements)

References 68 publications

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“…The absorbance at 490 nm was recorded using a Varioskan Flash microplate spectrophotometer. The cell viability was calculated according to the following equation normalc normale normall normall .25em normalv normali normala normalb normali normall normali normalt normaly .25em normalr normala normalt normale = ( A − A 0 ) / ( A c − A 0 ) × 100 % where A is the absorbance of the experimental sample, and A 0 and A c are the absorbance of blank group and control group, respectively. The data of cell viability rate represent as mean and standard deviation of three independent experiments.…”

Section: Experimental Sectionmentioning

confidence: 99%

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Mitochondrial-Targeted AIE-Active Fluorescent Probe Based on Tetraphenylethylene Fluorophore with Dual Positive Charge Recognition Sites for Monitoring ATP in Cells

et al. 2023

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Adenosine triphosphate (ATP), as an important intracellular energy currency produced in mitochondria, is closely related to various diseases in living organisms. Currently, the biological application of AIE fluorophore as a fluorescent probe for ATP detection in mitochondria is rarely reported. Herein, D−π−A and D−A structure-based tetraphenylethylene (TPE) fluorophores were employed to synthesize six different ATP probes (P1−P6), and the phenylboronic acid groups and dual positive charge sites of probes could interact with the vicinal diol of ribose and negatively charged triphosphate structure of ATP, respectively. However, P1 and P4 with a boronic acid group and a positive charge site had poor selectivity for ATP detection. In contrast, P2, P3, P5, and P6 with dual positive charge sites exhibited better selectivity than P1 and P4. In particular, P2 had more advantages of high sensitivity, selectivity, and good time stability for ATP detection than P3, P5, and P6, which was ascribed to its D−π−A structure, linker 1 (1,4-bis(bromomethyl)benzene), and dual positive charge recognition sites. Then, P2 was employed to detect ATP, and it exhibited a low detection limit of 3.62 μM. Moreover, P2 showed utility in the monitoring of mitochondrial ATP level fluctuations.

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Section: Experimental Sectionmentioning

confidence: 99%

“…The absorbance at 490 nm was recorded using a Varioskan Flash microplate spectrophotometer. The cell viability was calculated according to the following equation 29…”

Section: Synthesis Of P2mentioning

confidence: 99%

Mitochondrial-Targeted AIE-Active Fluorescent Probe Based on Tetraphenylethylene Fluorophore with Dual Positive Charge Recognition Sites for Monitoring ATP in Cells

et al. 2023

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“…Wang et al 63 designed probe 36 with combined AIE and ESIPT mechanisms for fast and selective sensing and imaging HOCl (ClO − ) (Fig. 30).…”

Section: Introductionmentioning

confidence: 99%

ESIPT-based probes for cations, anions and neutral species: recent progress, multidisciplinary applications and future perspectives

Nehra,

Kaushik

2023

Anal. Methods

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“…Fluorescent probes find widespread application in detection and labeling techniques, including the determination of biomolecular content, metal ions, pesticide residues as well as the tracing of molecules [ 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 ]. Fluorescence probes based on excited-state intramolecular proton transfer (ESIPT) have been developed and designed [ 11 , 12 , 13 , 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

Paying Comprehensive Attention to the Temperature-Dependent Dual-Channel Excited-State Intramolecular Proton Transfer Mechanism of Fluorescence Ratio Probe BZ-DAM

Gao,

Zhang,

et al. 2023

IJMS

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The mechanism of fluorescence detection of diethyl chlorophosphate (DCP) based on 2-substituted benzothiazole (BZ-DAM) was studied by a theoretical calculation method. It should not be ignored that both the BZ-DAM and the detection product BZ-CHO have two excited-state intramolecular proton transfer (ESIPT) channels. Density functional theory (DFT) and time-dependent DFT (TDDFT) theory were used to study the photophysical mechanism of two compounds in two channels in (acetonitrile) ACN solvent, and the temperature dependence of the two channels was given. Channel 1 is more likely to exist at low temperatures and channel 2 is more likely to exist at high temperatures. By theoretical analysis of the constructed potential energy curve, the hydrogen bond energy and electron-hole analysis, we confirmed that both molecules undergo ESIPT and intramolecular charge transfer (ICT) processes in channel 1 and ESIPT and twisted intramolecular charge transfer (TICT) coupling processes in channel 2. The formation of product BZ-CHO molecules led to a significant fluorescence blue-shift phenomenon and inhibited the ICT process, which confirmed that BZ-DAM could be used as a fluorescence probe for fluorescence detection. We sincerely hope that this work will not only help to clarify the excited-state dynamics behavior of the BZ-DAM probe but also provide a new idea for designing and optimizing a new chemical dosimeter.

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Tracking HOCl by an incredibly simple fluorescent probe with AIE plus ESIPT in vitro and in vivo

Cited by 9 publications

References 68 publications

Mitochondrial-Targeted AIE-Active Fluorescent Probe Based on Tetraphenylethylene Fluorophore with Dual Positive Charge Recognition Sites for Monitoring ATP in Cells

Mitochondrial-Targeted AIE-Active Fluorescent Probe Based on Tetraphenylethylene Fluorophore with Dual Positive Charge Recognition Sites for Monitoring ATP in Cells

ESIPT-based probes for cations, anions and neutral species: recent progress, multidisciplinary applications and future perspectives

Paying Comprehensive Attention to the Temperature-Dependent Dual-Channel Excited-State Intramolecular Proton Transfer Mechanism of Fluorescence Ratio Probe BZ-DAM

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