Tracking of Multimodal Therapeutic Nanocomplexes Targeting Breast Cancer in Vivo

Abstract

Nanoparticle-based therapeutics with local delivery and external electromagnetic field modulation holds extraordinary promise for soft-tissue cancers such as breast cancer; however, knowledge of the distribution and fate of nanoparticles in vivo is crucial for clinical translation. Here we demonstrate that multiple diagnostic capabilities can be introduced in photothermal therapeutic nanocomplexes by simultaneously enhancing both near-infrared fluorescence and magnetic resonance imaging (MRI). We track nanocomplexes in vivo, examining the influence of HER2 antibody targeting on nanocomplex distribution over 72 h. This approach provides valuable, detailed information regarding the distribution and fate of complex nanoparticles designed for specific diagnostic and therapeutic functions.

Optically Modulated Theranostic Nanoparticles

“…In recent work, Bardhan et al and Chen et al have demonstrated the modifications of silica-coated gold nanoshells for simultaneous enhancement of NIR fluorescence and T2-weighted MR contrast, and shown molecular targeting of breast, ovarian, and pancreatic cancers in mouse models (Figure 1.4). [39][40][41] El-Sayed and co-workers have reported in vitro demonstration of gold nanorods as contrast agents for both molecular imaging and photothermal cancer treatment. 42 Gold nanorods were conjugated to anti-epidermal growth factor receptor (anti-EGFR) antibodies and then incubated with epithelial cell lines of both malignant and non-malignant nature: HaCaT (human keratinocytes), and two malignant cell lines, HOC 313 clone 8 and HSC 3 (human oral squamous cell carcinoma).…”

Morphological Changes in Transplanted Sarcoma S45 at Photodynamic Therapy Using Nanocomposites Based on Gold Nanorods

“…В ряде работ был предложен метод доставки лекарственных средств в составе поли-электролитных капсул на основе золотых наноча-стицах (ЗНЧ), разрушающихся под воздействием лазерного излучения и доставляющих лекарство к мишеням [17,18], или с помощью наночастиц, окруженных слоем полимерного наногеля [19,20]. Кроме того, в составе наноконъюгатов часто используют фотоактивные вещества [21,22] и пептиды, облегчающие внутриклеточное проникновение [23]. Таким образом, золотые наноструктуры с плазмонным резонансом весьма перспективны для селективной ФДТ онкологических и других заболеваний.…”

In vivo tomographic imaging with fluorescence and MRI using tumor-targeted dual-labeled nanoparticles

“…Recently, nanoparticle (NP)-based imaging has attracted considerable attention. NPs have been widely used as the imaging contrast agent for their potential for earlier diagnosis, 12 greater biocompatibility, and reduced toxicity. 13 To achieve enhanced specificity and sensitivity, tumor-targeted NPs have been developed, and their feasibility in tumortargeted imaging has been demonstrated in vivo.…”