Tracking of tubular molecules for scientific applications

Parvin, Bahram; Peng, Chenglin; Maestre, Palafox

doi:10.1109/34.400570

Cited by 13 publications

(5 citation statements)

References 15 publications

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“…One feature of the mammary microenvironment of particular interest is the basement membrane, a tubular structure that underlies and corresponds to the basal aspect of the mammary cells. The image analysis tool leverages a technique that was developed for the tracking of tubular molecules [12]. The result is shown in Figure 11.…”

Section: Low-dose Radiation Studymentioning

confidence: 99%

DeepView

Parvin

Taylor

Cong

et al. 1999

Proceedings of the 1999 ACM/IEEE Conference on Supercomputing

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This paper outlines the requirements, architecture, and design of a "Microscopy Channel" over the wide area network. A microscopy channel advertises a listing of available online microscopes, where users can seamlessly participate in an experiment, acquire expert opinions, collect and process data, and store this information in their electronic notebook. The proposed channel is a collaborative problem solving environment (CPSE) that allows for both synchronous and asynchronous collaboration.Our testbed includes several unique electron and optical microscopes with applications ranging from material science to cell biology. We have studied current commercial CORBA services and concluded that three basic services are needed to meet the extensibility and functionality constraints. These include: Instrument Services (IS), Exchange Services (ES), and Computational Services (CS). These services sit on top of CORBA and its enabling services (naming, trading, security, and notification). IS provide a layer of abstraction for controlling any type of microscope. ES provide a common set of utilities for information management and transaction. CS provide the analytical capabilities needed for online microscopy and PSE.

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Section: Low-dose Radiation Studymentioning

confidence: 99%

DeepView

Parvin

Taylor

Cong

et al. 1999

Proceedings of the 1999 ACM/IEEE Conference on Supercomputing

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“…Due to the flourescence of the injected dye the retinal vessels appear bright in the angiography images (FA and ICG). Therefore, the vessels can be modeled as dark or bright tubes of varying diameters (see also [17] and [18]). Vessels with a discernible vascular wall are modeled as triple-tubes by combining three parallel tubes.…”

Section: A Anatomical and Pathological Featuresmentioning

confidence: 99%

Mapping the human retina

Pinz

Bernogger

Datlinger³

et al. 1998

IEEE Trans. Med. Imaging

195

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The new therapeutic method of scotoma-based photocoagulation (SBP) developed at the Vienna Eye Clinic for diagnosis and treatment of age-related macular degeneration requires retinal maps from scanning laser ophthalmoscope images. This paper describes in detail all necessary image analysis steps for map generation. A prototype software system for fully automatic map generation has been implemented and tested on a representative dataset selected from a clinical study with 50 patients. The map required for the SBP treatment can be reliably extracted in all cases. Thus, algorithms presented in this paper should be directly applicable in daily clinical routine without major modifications.

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“…Detection of precipitates is achieved by accumulating line segments as long as the convexity constraint i s satised. The actual search process is based on dynamic programming and the approach i s b e y ond the scope of this paper [7,8]. Examples of detection of DNA molecules and precipitates are shown in gure 3.…”

Section: Detectionmentioning

confidence: 99%

“…These are well known techniques in the eld of computer vision and we h a v e built on existing knowledge [1,7,8,6] to construct our system. In the context of remote microscopy, the system must provide the look and feel available to the local operator, and hide the inherent latency in the wide area network.…”

Section: Computational Techniquesmentioning

confidence: 99%

“…The actual software implementation uses threads to realize the necessary parallelism under the OSF operating system. The second mode of tracking is based on high level shape changes, where a variant o f t h e deformable model, based again on dynamic programming [7], is used to quantify and track objects of interest under the microscope. We use a multigrid implementation of this algorithm for maximum speed-up and higher tolerance for large motion.…”

Section: Tracking Subsystemmentioning

confidence: 99%

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Visual servoing for micro-manipulation

Parvin

Callahan²,

Maestre³

1996

Proceedings of 13th International Conference on Pattern Recognition

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A framework for automated micromanipulation of microscopic objects using visual information is developed and applied to diverse problems in biology and material science. These applications include microdissection of stretched and immobilized DNA molecules using an optical microscope and dynamic studies of crystal structure formation observed with a high power transmission electron microscope. Manipulation in this context refers not only to mechanical positioning or dissection of a specimen, but also to the variation of environmental parameters that elicit a response in the specimen. The common thread between these applications is a collection of computational techniques for image compression, autofocusing, object detection, tracking, and servo l o o p c o n trol. A second feature of our system is a distributed software architecture that allows the proposed dynamic experiments to be conducted remotely over the wide area network. In this context, the visual information processing is used to compensate for dynamic changes and the latencies in the wide area network. This system is now a v ailable over the global Internet.

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Tracking of tubular molecules for scientific applications

Cited by 13 publications

References 15 publications

DeepView

DeepView

Mapping the human retina

Visual servoing for micro-manipulation

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