Tracking single particle rotation: probing dynamics in four dimensions

Anthony, Stephen M.; Yu, Yan

doi:10.1039/c5ay00522a

Cited by 28 publications

(33 citation statements)

References 82 publications

(171 reference statements)

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“…Among them, the most widely used are rod-shaped particles, including gold nanorods (Gu et al, 2012, 2011; Neupane et al, 2016; Chaudhari and Pradeep, 2014; Xiao et al, 2011a), colloidal ellipsoids (Han et al, 2006; Mukhija and Solomon, 2007), quantum rods (Tsay et al, 2006; Ohmachi et al, 2012), filamentous viruses (Lettinga et al, 2005), and dumbbell particles (Uspal et al, 2013; Uspal and Doyle, 2014), Typically, the rotation of rod-shaped imaging probes can be measured along two of the three possible rotational axes: in-plane rotational angle φ and out-of-plane rotational angle θ (Fig. 1a) (Anthony and Yu, 2015). The third axis of rotation, longitudinal rotational angle δ around the long axis of a rod, is generally not visible unless optical anisotropy is created along the side of the rod.…”

Section: General Methods For Probing Rotational Dynamicsmentioning

confidence: 99%

“…However, a growing number of studies show that rotational dynamics are a prevalent and important feature in biological systems (Anthony and Yu, 2015). Noji et al (1997) performed an early study that directly observed the rotation of mitochondrial F 1 -ATPase during ATP hydrolysis.…”

Section: Introductionmentioning

confidence: 99%

“…A major challenge is the lack of methods to precisely and directly measure rotation reliably in a variety of different cellular environments (Anthony and Yu, 2015). Currently existing techniques for measuring rotational dynamics in biological systems rely on the use of anisotropic particles as imaging probes.…”

Section: Introductionmentioning

confidence: 99%

“…The former type includes colloidal rods (Han et al, 2006; Mukhija and Solomon, 2007), filamentous viruses (Lettinga et al, 2005), gold nanorods (Gu et al, 2013), and quantum rods (Tsay et al, 2006; Ohmachi et al, 2012). The latter includes particles with surface-tethered markers (Kukura et al, 2009; Robbins and Theriot, 2003; Irmscher et al, 2012) and two-faced Janus particles that have different optical properties on opposite sides (Anthony and Yu, 2015; Behrend et al, 2004a,b, 2005; Anker and Kopelman, 2003; Anthony et al, 2006, 2008; Hong et al, 2006). In this review, we assess these techniques for measuring the rotational dynamics in biological systems.…”

Section: Introductionmentioning

confidence: 99%

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Seeing the unseen: Imaging rotation in cells with designer anisotropic particles

Gao

Sanchez

2017

Micron

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Cellular functions are enabled by cascades of transient biological events. Imaging and tracking the dynamics of these events have proven to be a powerful means of understanding the principles of cellular processes. These studies have typically focused on translational dynamics. By contrast, investigations of rotational dynamics have been scarce, despite emerging evidence that rotational dynamics are an inherent feature of many cellular processes and may also provide valuable clues to understanding those cell functions. Such studies have been impeded by the limited availability of suitable rotational imaging probes. This has recently changed thanks to the advances in the development of anisotropic particles for rotational imaging. In this review, we will summarize current techniques for imaging rotation using particle probes that are anisotropic in shape or optical properties. We will highlight two studies that demonstrate how these techniques can be applied to explore important facets of cellular functions.

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Section: General Methods For Probing Rotational Dynamicsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Seeing the unseen: Imaging rotation in cells with designer anisotropic particles

Gao

Sanchez

2017

Micron

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“…A summary of these rotational imaging methods can be found in a recent review from our group. 122 One strategy involves using Janus particles.…”

Section: Janus Particles As Imaging Probesmentioning

confidence: 99%

Janus particles for biological imaging and sensing

Sanchez

Gao

et al. 2016

Analyst

163

146

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Janus particles, named after the two-faced Roman god Janus, have different surface makeups, structures or compartments on two sides. This review highlights recent advances in employing Janus particles as novel analytical tools for live cell imaging and biosensing. Unlike conventional particles used in analytical science, two-faced Janus particles provide asymmetry and directionality, and can combine different or even incompatible properties within a single particle. The broken symmetry enables imaging and quantification of rotational dynamics, revealing information beyond what traditional measurements offer. The spatial segregation of molecules on the surface of a single particle also allows analytical functions that would otherwise interfere with each other to be decoupled, opening up opportunities for novel multimodal analytical methods. We summarize here the development of Janus particles, a few general methods for their fabrication and, more importantly, the emerging and novel applications of Janus particles as multi-functional imaging probes and sensors.

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Nanoparticle Transport Through Polymers and Along Interfaces

Boyle

Maguire

Rose

et al. 2022

Macromolecular Engineering

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Transport of nanoparticles has received increasing attention for both fundamental interest to test prevailing models as well as practical interest in separations and self‐healing applications. Methods for measuring particle dynamics are briefly reviewed to orient the reader to different methodologies. The transport of nanoparticles through polymer melts, solutions, and cross‐linked networks is described with emphasis on systems where the nanoparticle is on the length scale of the defining structure including the tube size, correlation length, and mesh size, respectively. Because nanoparticles can interact with the different mediums, the interactions between nanoparticles and polymer and the subsequent impacts on dynamics are described. The transport of particles at liquid–solid (polymer brush) interfaces is addressed due to growing interest in applications ranging from understanding dynamics in porous media to the translocation of DNA. Both hard particles and polymer grafted particles (soft particles) are described. The objective of this article is to provide the reader with fundamental descriptions of phenomena while reviewing the current state of understanding. Further directions will leverage advances in inorganic chemistry to prepare monodisperse, functional nanoparticles as well as polymer chemistry to create novel media, such as networks with monodisperse mesh size.

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Tracking single particle rotation: probing dynamics in four dimensions

Cited by 28 publications

References 82 publications

Seeing the unseen: Imaging rotation in cells with designer anisotropic particles

Seeing the unseen: Imaging rotation in cells with designer anisotropic particles

Janus particles for biological imaging and sensing

Nanoparticle Transport Through Polymers and Along Interfaces

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