Tracking the Single-Carbon-Dot Transmembrane Transport by Force Tracing Based on Atomic Force Microscopy

Lu, Denghua; Yang, Xudong; Zhang, Qingrong; Wang, Ruixia; Zhou, Siyuan; Yang, Guocheng; Shan, Yuping

doi:10.1021/acsbiomaterials.8b01363

Cited by 10 publications

(9 citation statements)

References 42 publications

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“…Subsequently, the AFM tip was slowly moved to the contact point and slightly brought into contact with the cell surface via the AFM feedback system, and then the feedback system was turned off. 12 The diagram of the force tracing setup is shown in Figure 1B, the photodetector will record the change of laser position caused by the cantilever deflection.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…Taking the advantages of high temporal− spatial resolution and single-particle tracking under physiological conditions, the force tracing technique on the basis of atomic force microscopy (AFM) has proven to be an excellent method for studying the transmembrane dynamic mechanism. 12 Force tracing could detect force as low as 10 pN and track fast processes down to 10 μs, which is much faster than other force testing techniques (for example, magnetic forceps, 13 optical tweezers, 14 and glass microneedles 15 ). The force tracing technique has been used to study the differences in transmembrane transporting dynamic parameters of gold nanoparticles (Au NPs, 5 nm, 10 nm, and 20 nm).…”

Section: ■ Introductionmentioning

confidence: 99%

“…The transmembrane transport of Au NCs is a dynamic process, and one technique with higher temporal–spatial resolution is necessary to track the dynamic process. Taking the advantages of high temporal–spatial resolution and single-particle tracking under physiological conditions, the force tracing technique on the basis of atomic force microscopy (AFM) has proven to be an excellent method for studying the transmembrane dynamic mechanism . Force tracing could detect force as low as 10 pN and track fast processes down to 10 μs, which is much faster than other force testing techniques (for example, magnetic forceps, optical tweezers, and glass microneedles).…”

Section: Introductionmentioning

confidence: 99%

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Size-Dependent Transmembrane Transport of Gold Nanocages

et al. 2020

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Gold nanocages (Au NCs), as drug carriers, have been widely applied for cancer diagnosis and photothermal therapy (PTT). Transmembrane transporting efficacy of Au NCs is the fundamental and important issue for their use in PTT. Herein, we used a force tracing technique based on atomic force microscopy to track the dynamic transmembrane process of Au NCs at the single-particle level in real time. Meanwhile, we measured and compared the dynamic parameters of Au NCs with sizes of 50 and 100 nm usually used as nanodrug carriers of PTT. It is concluded that the 50 nm Au NC transmembrane transporting needs smaller force and shorter duration with a much faster speed. However, both the 50 and 100 nm Au NC transmembrane transporting depends on the caveolin-mediated endocytosis, clathrin-mediated endocytosis, and macropinocytosis, which was also confirmed by confocal fluorescence imaging. This report will provide a potential technique for screening nanodrug carriers from the perspective of transmembrane transporting efficacy.

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Section: ■ Results and Discussionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Size-Dependent Transmembrane Transport of Gold Nanocages

et al. 2020

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“…Fortunately, the size control method of most colloidal materials is mature nowadays, and sub-30-nm-sized NPs, including UCNPs (Liu et al, 2017;Zhan et al, 2017), SiNPs (Liang et al, 2020), AIE dots (Fang et al, 2017;Li et al, 2017;Li et al, 2018), PDots (Wu et al, 2020), and QDots (Lesoine et al, 2013;Hanne et al, 2015;Ye et al, 2018;Zhao et al, 2019), have all been successfully fabricated for STED applications, some of which are even commercially available (Hanne et al, 2015;Zhao et al, 2019). CDots (Leménager et al, 2014;Wang et al, 2017;Han et al, 2019;Hua et al, 2019;Li et al, 2019), on the contrary, are naturally nano-sized (<10 nm) and are well known for their fast transport dynamics across membranes (Lu et al, 2019). For FNDs and LPR nanohybrid particles, the smallest particle size in reported STED imaging cases is 40∼50 nm (Tzeng et al, 2011;Urban et al, 2018).…”

Section: Particle Sizementioning

confidence: 99%

Shedding New Lights Into STED Microscopy: Emerging Nanoprobes for Imaging

et al. 2021

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First reported in 1994, stimulated emission depletion (STED) microscopy has long been regarded as a powerful tool for real-time superresolved bioimaging . However, high STED light power (101∼3 MW/cm2) is often required to achieve significant resolution improvement, which inevitably introduces phototoxicity and severe photobleaching, damaging the imaging quality, especially for long-term cases. Recently, the employment of nanoprobes (quantum dots, upconversion nanoparticles, carbon dots, polymer dots, AIE dots, etc.) in STED imaging has brought opportunities to overcoming such long-existing issues. These nanomaterials designed for STED imaging show not only lower STED power requirements but also more efficient photoluminescence (PL) and enhanced photostability than organic molecular probes. Herein, we review the recent progress in the development of nanoprobes for STED imaging, to highlight their potential in improving the long-term imaging quality of STED microscopy and broadening its application scope. We also discuss the pros and cons for specific classes of nanoprobes for STED bioimaging in detail to provide practical references for biological researchers seeking suitable imaging kits, promoting the development of relative research field.

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“…3d), which may be attributed to the larger molecule structure of EGF and the more complex EGF–EGFR receptor-mediated endocytosis. 25 However, the probability of an observed force–time signal is lower (6.9%) than that of PAMAM-CPT-EGF (13.6%), indicating the higher transporting efficiency of PAMAM-CPT-EGF. Likewise, Wei-Ting Kuo et al also concluded that EGF has a higher adhesion force and binding affinity to EGFR.…”

mentioning

confidence: 93%