Tracking the Story of Cytokinin Research

Kamínek, M.

doi:10.1007/s00344-015-9543-4

Cited by 29 publications

(17 citation statements)

References 147 publications

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“…Starting with physiology, his work has extended to analytical methods and biochemistry later. His story on the history of cytokinins (Kaminek 2015) and so on to complete the individual reviews. Certainly, there are much more aspects in the history of plant hormones that could have been covered, but we do hope that our readers will appreciate as much as we do the interesting and dedicated contributions in this special issue.…”

Section: A Special Issue On the History Of Plant Hormone Researchmentioning

confidence: 99%

From Facts and False Routes: How Plant Hormone Research Developed

Ludwig‐Müller

Lüthen

2015

J Plant Growth Regul

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Our understanding of how plant hormones work has tremendously increased over the last decades. However, such progress was not possible without those who laid the foundation of the work which was sometimes much more tedious in early days than today. Therefore, we thought it timely to follow-up on historical aspects of plant hormone research. The development in the field of auxins, cytokinins, gibberellins, abscisic acid, jasmonates, brassinosteroids, and ethylene are accompanied with personal recollections. Plant hormones cannot go without plant growth regulators which have been developed for commercial reasons, but are also used in many cases nowadays as tools to investigate a specific role of a hormone in planta. This issue is also hopefully a useful collection for those scientists who want to get an overview of how their ''favorite hormone'' started.

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Section: A Special Issue On the History Of Plant Hormone Researchmentioning

confidence: 99%

From Facts and False Routes: How Plant Hormone Research Developed

Ludwig‐Müller

Lüthen

2015

J Plant Growth Regul

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“…CKs are divided into two groups depending on the structure of the N 6 substituent: (i) aliphatic or isoprenoid, including N 6 -isopentenyladenine (iP) and zeatins ( Figure 1A), and (ii) aromatic, including N 6 -benzyladenine (BA), topolins, and N 6 -furfuryladenine (kinetin) ( Figure 1B) [1,2]. Since the discovery of kinetin in 1955 by Miller and Skoog [3], a large number of various CKs as well as corresponding nucleoside and nucleotide derivatives were synthesized or isolated from different plant sources [4,5]. Among these numerous molecular forms, only CKs as nucleobases possess hormonal activity, but not their nucleoside or nucleotide derivatives [6,7].…”

Section: Introductionmentioning

confidence: 99%

“…Since the discovery of kinetin in 1955 by Miller, Skoog et al [3], a large number of various CKs as well as corresponding nucleoside and nucleotide derivatives were synthesized or isolated from different plant sources [4,5]. Among these numerous molecular forms, only CKs as nucleobases possess hormonal activity, but not their nucleoside or nucleotide derivatives [6,7].…”

Section: Introductionmentioning

confidence: 99%

“…To date, the CK biosynthesis pathways are not completely deciphered. The biosynthesis of isoprenoid CKs, in particular iP, is most studied, while the biosynthesis pathway of aromatic CKs is practically unknown [5,8]. The key step of the iP biosynthesis is commonly assumed to be isopentenyl transferase-catalyzed transfer of isopentenyl moiety from dimethylallyl diphosphate (DMAPP) to the N 6 position of adenosine-5 -monophosphate (AMP), adenosine-5 -diphosphate (ADP) or adenosine-5 -triphosphate (ATP), with the formation of the respective 5 -nucleotides (iPRMP, iPRDP, iPRTP) ( Figure 2).…”

Section: Introductionmentioning

confidence: 99%

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Distinct Peculiarities of In Planta Synthesis of Isoprenoid and Aromatic Cytokinins

et al. 2020

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The biosynthesis of aromatic cytokinins in planta, unlike isoprenoid cytokinins, is still unknown. To compare the final steps of biosynthesis pathways of aromatic and isoprenoid cytokinins, we synthesized a series of nucleoside derivatives of natural cytokinins starting from acyl-protected ribofuranosyl-, 2′-deoxyribofuranosyl- and 5′-deoxyribofuranosyladenine derivatives using stereoselective alkylation with further deblocking. Their cytokinin activity was determined in two bioassays based on model plants Arabidopsis thaliana and Amaranthus caudatus. Unlike active cytokinins-bases, cytokinin nucleosides lack the hormonal activity until the ribose moiety is removed. According to our experiments, ribo-, 2′-deoxyribo- and 5′-deoxyribo-derivatives of isoprenoid cytokinin N6-isopentenyladenine turned in planta into active cytokinins with clear hormonal activity. As for aromatic cytokinins, both 2′-deoxyribo- and 5′-deoxyribo-derivatives did not exhibit analogous activity in Arabidopsis. The 5′-deoxyribo-derivatives cannot be phosphorylated enzymatically in vivo; therefore, they cannot be “activated” by the direct LOG-mediated cleavage, largely occurring with cytokinin ribonucleotides in plant cells. The contrasting effects exerted by deoxyribonucleosides of isoprenoid (true hormonal activity) and aromatic (almost no activity) cytokinins indicates a significant difference in the biosynthesis of these compounds.

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“…The cytokinin (CK) plant hormones are organic compounds that are effective at very low concentrations (pmol g −1 fresh weight, FW) and play essential roles during plant growth and development. They promote cell division in the presence of auxin, inhibit apical dominance, slow down aging of plant tissues, increase resistance to stress and stimulate photosynthesis (Miller et al ., ; Mok and Mok, ; Kamínek, ). They are defined as N 6 ‐substituted derivatives of adenine, and can be divided into isoprenoid and aromatic CKs according to the character of their side chain.…”

Section: Introductionmentioning

confidence: 99%

Microscale magnetic microparticle‐based immunopurification of cytokinins from Arabidopsis root apex

et al. 2017

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Cytokinins (CKs) are pivotal plant hormones that have crucial roles in plant growth and development. However, their isolation and quantification are usually challenging because of their extremely low levels in plant tissues (pmol g fresh weight). We have developed a simple microscale magnetic immunoaffinity-based method for selective one-step isolation of CKs from very small amounts of plant tissue (less than 0.1 mg fresh weight). The capacity of the immunosorbent and the effect of the complex plant matrix on the yield of the rapid one-step purification were tested using a wide range of CK concentrations. The total recovery range of the new microscale isolation procedure was found to be 30-80% depending on individual CKs. Immunoaffinity extraction using group-specific monoclonal CK antibodies immobilized onto magnetic microparticles was combined with a highly sensitive ultrafast mass spectrometry-based method with a detection limit close to one attomole. This combined approach allowed metabolic profiling of a wide range of naturally occurring CKs (bases, ribosides and N -glucosides) in 1.0-mm sections of the Arabidopsis thaliana root meristematic zone. The magnetic immunoaffinity separation method was shown to be a simple and extremely fast procedure requiring minimal amounts of plant tissue.

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Tracking the Story of Cytokinin Research

Cited by 29 publications

References 147 publications

From Facts and False Routes: How Plant Hormone Research Developed

From Facts and False Routes: How Plant Hormone Research Developed

Distinct Peculiarities of In Planta Synthesis of Isoprenoid and Aromatic Cytokinins

Microscale magnetic microparticle‐based immunopurification of cytokinins from Arabidopsis root apex

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