Tradescantia as a biomonitor for genotoxicity evaluation of diesel and biodiesel exhaust emissions

Placencia, Fabián; Fadic, Ximena; Yáñez, Karen; Cereceda-Balic, Francisco

doi:10.1016/j.scitotenv.2018.10.009

Cited by 11 publications

(4 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is known that gaseous PAHs, carbonyls, and other gaseous compounds such as NO 2 can generate DNA damage in cells either directly or indirectly, for example, by oxidative stress [74][75][76]. Based on our results, we suggest that gaseous molecules should be given more attention in future studies.…”

Section: Genotoxicitymentioning

confidence: 52%

Influence of wood species on toxicity of log-wood stove combustion aerosols: a parallel animal and air-liquid interface cell exposure study on spruce and pine smoke

et al. 2020

View full text Add to dashboard Cite

Background: Wood combustion emissions have been studied previously either by in vitro or in vivo models using collected particles, yet most studies have neglected gaseous compounds. Furthermore, a more accurate and holistic view of the toxicity of aerosols can be gained with parallel in vitro and in vivo studies using direct exposure methods. Moreover, modern exposure techniques such as air-liquid interface (ALI) exposures enable better assessment of the toxicity of the applied aerosols than, for example, the previous state-of-the-art submerged cell exposure techniques. Methods: We used three different ALI exposure systems in parallel to study the toxicological effects of spruce and pine combustion emissions in human alveolar epithelial (A549) and murine macrophage (RAW264.7) cell lines. A wholebody mouse inhalation system was also used to expose C57BL/6 J mice to aerosol emissions. Moreover, gaseous and particulate fractions were studied separately in one of the cell exposure systems. After exposure, the cells and animals were measured for various parameters of cytotoxicity, inflammation, genotoxicity, transcriptome and proteome.

show abstract

Section: Genotoxicitymentioning

confidence: 52%

Influence of wood species on toxicity of log-wood stove combustion aerosols: a parallel animal and air-liquid interface cell exposure study on spruce and pine smoke

et al. 2020

View full text Add to dashboard Cite

show abstract

“…For example, the Trad-SHM and Trad-MN test systems with Tradescantia clone 02 showed toxic effects, even when most of the measured toxicants in a lake were below the WHO standards for drinking water quality [29], suggesting high sensitivity to low contaminant concentrations [25] or high performance in the detection of the potential toxicity of a contaminant mix. T. pallida was employed in a 2 h exposure to diesel and biodiesel exhaust emissions, demonstrating higher genotoxicity, consistent with higher concentrations of particulate matter, carbonyls and PAH content [28]. In this work, for the first time, the genotoxic potential of a HAB event was assessed using two different Tradescantia-based test systems on HAB in Lake Sevan (Armenia) in July 2020.…”

Section: Introductionmentioning

confidence: 90%

“…Microtox (R) testing detected toxicity in the samples collected during cyanobacteria, dinoflagellate and haptophyte blooms [24]. However, no studies used the Tradescantia test for the evaluation of HABs' potential toxicity, although the Tradescantia-based test systems are known for their high sensitivity to even low concentrations of contaminants and wide applicability for a variety of risk assessment purposes (air, water, soils) [25][26][27][28]. For example, the Trad-SHM and Trad-MN test systems with Tradescantia clone 02 showed toxic effects, even when most of the measured toxicants in a lake were below the WHO standards for drinking water quality [29], suggesting high sensitivity to low contaminant concentrations [25] or high performance in the detection of the potential toxicity of a contaminant mix.…”

Section: Introductionmentioning

confidence: 99%

Tradescantia-Based Test Systems Can Be Used for the Evaluation of the Toxic Potential of Harmful Algal Blooms

Khosrovyan

Avalyan

Atoyants

et al. 2023

Water

View full text Add to dashboard Cite

Harmful algal blooms (HABs) are overgrowths of toxic strains of algae (diatoms, green) and cyanobacteria (blue-green algae). While occurring naturally, human-induced environmental changes have resulted in more frequent occurrences of such blooms worldwide. Meantime, the ecotoxicological risk of HABs is rarely evaluated by means of standard test methods. For the first time, the genotoxic potential of the HAB event 2020 was assessed using two different Tradescantia-based test systems (Trad-SHM and Trad-MN, 24-h exposure). An integrated analysis of biological (algal abundance) and ecotoxicological (testing) data revealed linkages among algal proliferation, changes in Tradescantia stamen hairs (mutations and suppressed growth) and chromosomal aberrations during microsporogenesis (appearance of micronuclei) that were likely to be caused by toxic algal groups. Green alga Botryococcus braunii and the cyanobacterial species Anabaena and Oscillatoria could suppress stamen hair growth; Cyanobacteria Phormidium and Aphanothece sp. could trigger mutations in stamen hairs (appearance of pink and colorless cells); and Oscillatoria sp. could be responsible for the occurrence of chromosomal damage. Diatom proliferation in the spring was not related to the genotoxic response in Tradescantia. Both tests, the Trad-SHM and Trad-MN, are suitable for the evaluation of the toxic potential of HABs.

show abstract

“…The induction of SOS DNA repair system in E. coli K-12 mutants on exposure of DNA damaging agents is another assay for genotoxicity testing ( Ferná ndez de Henestrosa et al., 2000 ; Maslowska et al., 2015 ). Several plant systems such as Allium cepa , Vigna radiata , Vicia faba , Tradescantia , Zea mays and Nicotiana tabacum have been used as an indicator of toxicity of industrial wastewater ( Han et al., 2011 ; Iqbal and Nisar 2015 ; Haq et al., 2016 ; Iqbal 2016 ; Placencia et al., 2019 ). Out of these, Allium cepa and Vigna radiata are commonly used in monitoring of genotoxic potential due to their ability to detect different endpoints e.g.…”

Section: Introductionmentioning

confidence: 99%

Cyto-genotoxic potential of petroleum refinery wastewater mixed with domestic sewage used for irrigation of food crops in the vicinity of an oil refinery

Khatoon¹,

Malik²

2021

Heliyon

View full text Add to dashboard Cite

Petroleum refinery wastewater combined with domestic sewage were collected from the open channel in the vicinity of Mathura oil refinery, UP (India) and analysed by inductively coupled plasma optical emission spectrometry (ICP-OES) and gas chromatography-mass spectrometry (GC-MS) for elemental analysis and organic pollutants, respectively. Several potentially toxic and non-toxic elements were found to be present in the wastewater samples. GC-MS analysis revealed the presence of several organic contaminants including pesticides. Wastewater samples were extracted using amberlite XAD4/8 resins and liquid-liquid extraction procedures using different organic solvents. The extracts were tested for their cyto-genotoxic potential using bacterial (Salmonella mutagenicity test, E. coli K-12 DNA repair defective mutants, Bacteriophage λ assay) and plant (Vigna mungo phytotoxicity test, Allium cepa chromosomal aberration assay) systems. A significant increase was observed in the number of revertants of TA97a, TA98 and TA100 strains with the test samples and XAD concentrated samples were found to be more mutagenic than liquid-liquid extracts. Colony forming units (CFUs) of DNA repair defective mutants of E. coli K-12 recA, lexA and polA declined significantly as compared to their isogenic wild-type counterparts with the test samples. Significant reduction in plaque forming units (PFUs) of bacteriophage λ was also found on treatment with the solvent extracts. Presence of several toxic pollutants in the wastewater apply prohibitive action on the seed germination process. Germination rate of Vigna mungo seeds as well as radicle and plumule lengths were found to be affected when treated with different concentration of wastewater as compared to control. Present study also indicated concentration dependent reduction in mitotic index of A. cepa i.e., 16.38% at 5% and 9.74% at 100% wastewater and percentage of aberrant cells were highest at 100% effluent. Present findings indicated that mutagenicity/genotoxicity of wastewater is due to the mixture of genotoxins; poses serious hazards to the receiving waterbodies which require continuous monitoring and remedial measures for their improvement.

show abstract

Tradescantia as a biomonitor for genotoxicity evaluation of diesel and biodiesel exhaust emissions

Cited by 11 publications

References 32 publications

Influence of wood species on toxicity of log-wood stove combustion aerosols: a parallel animal and air-liquid interface cell exposure study on spruce and pine smoke

Influence of wood species on toxicity of log-wood stove combustion aerosols: a parallel animal and air-liquid interface cell exposure study on spruce and pine smoke

Tradescantia-Based Test Systems Can Be Used for the Evaluation of the Toxic Potential of Harmful Algal Blooms

Cyto-genotoxic potential of petroleum refinery wastewater mixed with domestic sewage used for irrigation of food crops in the vicinity of an oil refinery

Contact Info

Product

Resources

About