Traditional Chinese medicine active ingredients-based selenium nanoparticles regulate antioxidant selenoproteins for spinal cord injury treatment

Abstract

Background As Traditional Chinese Medicine (TCM) drugs, Huangqi and Danshen are always applied in combination for spinal cord injury (SCI) treatment based on the compatibility theory of TCM. Astragalus Polysaccharidesis (APS) and Tanshinone IIA (TSIIA) are the main active ingredients of Huangqi and Danshen, and they both possess neuroprotective effects through antioxidant activities. However, low solubility and poor bioavailability have greatly limited their application. In recent years, selenium nanoparticles (SeNPs) have drawn enormous attention as potential delivery carrier for antioxidant drugs. Results In this study, TCM active ingredients-based SeNPs surface decorated with APS and loaded with TSIIA (TSIIA@SeNPs-APS) were successfully synthesized under the guidance of the compatibility theory of TCM. Such design improved the bioavailability of APS and TSIIA with the benefits of high stability, efficient delivery and highly therapeutic efficacy for SCI treatment illustrated by an improvement of the antioxidant protective effects of APS and TSIIA. The in vivo experiments indicated that TSIIA@SeNPs-APS displayed high efficiency of cellular uptake and long retention time in PC12 cells. Furthermore, TSIIA@SeNPs-APS had a satisfactory protective effect against oxidative stress-induced cytotoxicity in PC12 cells by inhibiting excessive reactive oxygen species (ROS) production, so as to alleviate mitochondrial dysfunction to reduce cell apoptosis and S phase cell cycle arrest, and finally promote cell survival. The in vivo experiments indicated that TSIIA@SeNPs-APS can protect spinal cord neurons of SCI rats by enhancing GSH-Px activity and decreasing MDA content, which was possibly via the metabolism of TSIIA@SeNPs-APS to SeCys2 and regulating antioxidant selenoproteins to resist oxidative stress-induced damage. Conclusions TSIIA@SeNPs-APS exhibited promising therapeutic effects in the anti-oxidation therapy of SCI, which paved the way for developing the synergistic effect of TCM active ingredients by nanotechnology to improve the efficacy as well as establishing novel treatments for oxidative stress-related diseases associated with Se metabolism and selenoproteins regulation.

Network Pharmacology and Molecular Docking-Based Investigation of Potential Targets of Astragalus membranaceus and Angelica sinensis Compound Acting on Spinal Cord Injury

“…A recent study found that AM can inhibit the expression of glial fibrillary acidic protein (GFAP) by increasing SOD activity, thereby preventing or reducing secondary SCI. Moreover, high-dose AM injection can reduce the degree of spinal cord edema and improve spinal nerve function with rat spinal cord injury [ 11 ]. Angelica sinensis (Dang-gui, AS) is the dried root of Angelica sinensis (Oliv.…”

Novel insights into the synergistic effects of selenium nanoparticles and metformin treatment of letrozole - induced polycystic ovarian syndrome: targeting PI3K/Akt signalling pathway, redox status and mitochondrial dysfunction in ovarian tissue

“…Also, traditional Chinese medicine active ingredients-based SeNPs exhibited protective effects against oxidative stress-induced cytotoxicity in PC12 cells through alleviating mitochondrial dysfunction [ 57 ]. Moreover, MET treatment improved mitochondrial function in leukocytes of PCOS patients [ 58 ].…”

CoSe2 Nanoparticles with Multienzymic Activities for Antibacterial Applications

“…15 In addition, selenium plays an essential role as an antibacterial and antioxidant. 16,17 Recently, CoSe 2 was reported as a nanoenzyme with peroxidase (POD) mimicking activity in the detection of glutathione and dye degradation. 18,19 Similarly, a highly specific and sensitive colorimetric test for the detection of H 2 O 2 and glucose was established using CoSe 2 /reduced graphene oxide (CoSe 2 /rGO) nanohybrids, which were revealed to contain intrinsic POD-like catalytic activity.…”

“…Owing to excellent electrocatalytic activity, high availability, environmental friendliness, and structural and compositional advantages, CoSe 2 has attracted considerable attention for its potential to serve as a catalyst for hydrogen peroxide synthesis, hydrogen evolution reaction, oxygen evolution reaction, etc. − Cobalt catalysis shares similar reactivity to iron, , which have distinctive electrocatalysis and optical properties, , and are widely used in the battery storage material , and biological medicine. , Selenium is critical to the antioxidant activity of glutathione peroxidase and for cell-protective selenoproteins . In addition, selenium plays an essential role as an antibacterial and antioxidant. , …”