Trägersynthesen, 6. Träger‐Oligonucleotidsynthese an unvernetzten Copolymeren aus Vinylalkohol und N‐Vinylpyrrolidon

Abstract

zu ihrem 65. Geburtstag gewidmet ZUSAMMENFASSUNG:Unvernetzte Copolymere aus Vinylacetat und N-Vinylpyrrolidon wurden verseift und die erhaltenen Copolymeren als Trager fur eine Oligonucleotid-Synthese in homogener Phase eingesetzt. Desoxythymidin als Kettenanfang wurde uber eine Carbonyloxy-Brucke an den Trager angekniipft. Die Kettenverlangerung erfolgte zunachst durch Oligokondensation mit ungeschutztem Desoxythymidinphosphat, sodann durch Anbau einer geschutzten Nucleotideinheit an das 3'-Ende. Oligonucleotide des Typs dT (pdT), und dT (pdT)"-rU wurden auf diesem Wege gewonnen. SUMMARY:The saponification products of non-crosslinked copolymers from vinyl acetate and N-vinylpyrrolidone were used as supports for oligonucleotide synthesis in homogeneous phase. Desoxythymidineas initial nucleoside was attached to the carrier via an carbonyloxy linkage. The elongation of the oligonucleotide chain was achieved first by oligocondensation with unprotected desoxythymidine phosphate, followed by addition of a protected nucleotide unit at the 3'-end. Oligonucleotides of the types dT (pdT), and dT (pdT),-rU were obtained in this way. Einleitung

Organic Synthesis on Soluble Polymer Supports: Liquid-Phase Methodologies

“…A hydrolyzed copolymer of polyvinyl acetate and poly(1-vinyl-2-pyrrolidinone) of molecular weight 42 000 (10−20% vinyl alcohol) provided a soluble support containing hydroxyl anchoring sites and possessing higher solubilizing power than polyvinyl acetate. , Nucleotides were attached at the 5‘-hydroxyl group to the polymer by carbonate linkages with a loading capacity of 1 mmol/g. Oligothymidines of 1−4 residues in length were prepared; however, dialysis in water was insufficient for removal of impurities, a second step using ion chromatography was required to complete purification.…”