Trans-epithelial Fluid Pumping Performance of Renal Epithelial Cells and Mechanics of Cystic Expansion

Choudhury, Mohammad Ikbal; Li, Yizeng; Mistriotis, Panagiotis; Dixon, Eryn E.; Yang, Jing; Maity, Damodar; Walker, Rebecca; Benson, Morgen; Martin, Leigha; Koroma, Fatima; Qian, Feng; Κωνσταντόπουλος, Κωνσταντίνος; Woodward, Owen M.; Sun, Sean X.

doi:10.1101/727313

Cited by 4 publications

(1 citation statement)

References 19 publications

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“…The pumping action of NaK can cause an imbalance of ions at the cell leading/trailing edge, generating water influx/efflux driven by the osmotic pressure gradient. NaK has been shown to generate water fluxes 25,26 . Upon inhibition of NaK by Ouabain 23,27 , we observed a reduction in cell speed for both control and high viscosity media.…”

Section: Water Permeation Plays a Significant Role In Cell Motility I...mentioning

confidence: 99%

Extracellular Hydraulic Resistance Enhances Cell Migration

Maity

Bera

et al. 2022

Advanced Science

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Cells migrating in vivo can encounter microenvironments with varying physical properties. One such physical variable is the viscosity of the fluid surrounding the cell. Increased fluid viscosity is expected to increase the hydraulic resistance experienced by the migrating cell and therefore decrease the cell speed.We demonstrate that contrary to this expected result, cells migrate faster in high viscosity media on 2D substrates. To reveal the molecular mechanism, we examined both actin dynamics and water dynamics driven by ion channel activity. Results show that cells increased in area in high viscosity and actomyosin dynamics remained similar, except that actin retrograde flow speed is reduced. Inhibiting ion channel fluxes in high viscosity media results in a large reduction in cell speed, suggesting that water flux contributes to the observed speed increase. Moreover, inhibiting actin-dependent vesicular trafficking that transports ion channels from the ER to the cell boundary changes ion channel spatial positioning and reduces cell speed in high viscosity media. Cells also displayed altered Ca 2+ -activity in high viscosity media, and when cytoplasmic Ca 2+ is sequestered, cell speed reduction and altered ion channel positioning were observed.Taken together, we find that the cell cytoplasmic actin-phase and water-phase are coupled during cell migration in high viscosity media. Directional water fluxes are mediated by ion channels whose position depend on actin-based vesicular trafficking. There are no significant changes in ion channel total content in high viscosity, in agreement with physical modeling that also predicts the observed cell speedup in high viscosity environment.

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Section: Water Permeation Plays a Significant Role In Cell Motility I...mentioning

confidence: 99%

Extracellular Hydraulic Resistance Enhances Cell Migration

Maity

Bera

et al. 2022

Advanced Science

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Microscale pressure measurements based on an immiscible fluid/fluid interface

Yang

Duan

Fraser

et al. 2019

Sci Rep

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A method of microscale pressure measurement based on immiscible fluid/fluid interface is proposed. This method utilizes observed curvature changes in a fluid/fluid interface, and can accurately report hydraulic pressure in fluids at length scales of 10 microns. The method is especially suited for measuring fluid pressure in micro-scale biological samples. Using this method, we probe fluid pressure build up in epithelial domes, murine mammary gland organoids embedded in hydrogel, and lumen pressure in the developing mouse embryo. Results reveal that the pressure developed across epithelial barriers is on the order of 100~300 Pa, and is modulated by ion channel activity.

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The importance of water and hydraulic pressure in cell dynamics

Κωνσταντόπουλος

Zhao

et al. 2020

Journal of Cell Science

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All mammalian cells live in the aqueous medium, yet for many cell biologists, water is a passive arena in which proteins are the leading players that carry out essential biological functions. Recent studies, as well as decades of previous work, have accumulated evidence to show that this is not the complete picture. Active fluxes of water and solutes of water can play essential roles during cell shape changes, cell motility and tissue function, and can generate significant mechanical forces. Moreover, the extracellular resistance to water flow, known as the hydraulic resistance, and external hydraulic pressures are important mechanical modulators of cell polarization and motility. For the cell to maintain a consistent chemical environment in the cytoplasm, there must exist an intricate molecular system that actively controls the cell water content as well as the cytoplasmic ionic content. This system is difficult to study and poorly understood, but ramifications of which may impact all aspects of cell biology from growth to metabolism to development. In this Review, we describe how mammalian cells maintain the cytoplasmic water content and how water flows across the cell surface to drive cell movement. The roles of mechanical forces and hydraulic pressure during water movement are explored.

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Trans-epithelial Fluid Pumping Performance of Renal Epithelial Cells and Mechanics of Cystic Expansion

Cited by 4 publications

References 19 publications

Extracellular Hydraulic Resistance Enhances Cell Migration

Extracellular Hydraulic Resistance Enhances Cell Migration

Microscale pressure measurements based on an immiscible fluid/fluid interface

The importance of water and hydraulic pressure in cell dynamics

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