Transaminase in seminal plasma of man

Povoa, H; Villela, Gilberto G.

doi:10.1007/bf02178987

Cited by 17 publications

(5 citation statements)

References 5 publications

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“…The levels of GOT activity present in human seminal plasma measured in the present study (Table 1) compare well with several previously reported studies [15, 181, but are slightly higher than those in another series [14], although this last group of workers used a less sensitive colorimetric enzyme assay method. Comparisons with other studies [6, 91 are precluded since in one study [9] the temperature at which the enzyme activities were determined was not given and in another [6] the author stated that in most cases the semen was stored at -20°C for up to 2 weeks before preparation of the cell-free supernatant for assay-a procedure that would …”

Section: Discussionsupporting

confidence: 92%

“…The levels of glutamic-oxalacetic transaminase (GOT: EC 2.6.1.1) activity in human semen have been reported to be some ten to twenty times that found in normal blood plasma [8, 14,181. While much of this activity appears to be derived from the secretion of the prostate gland [5], the levels of GOT in the semen also show a positive correlation with sperm density [9,14,151.…”

Section: Introductionmentioning

confidence: 99%

“…While much of this activity appears to be derived from the secretion of the prostate gland [5], the levels of GOT in the semen also show a positive correlation with sperm density [9,14,151. Release of GOT has been used as an indicator of cell damage during cryostorage of spermatozoa in domestic animals (bull: 13; boar: 1-3, 7,11 ram: 17).…”

Section: Introductionmentioning

confidence: 99%

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Glutamic-Oxalacetic Transaminase Leakage from Human Spermatozoa as an Indicator of Cryodamage

Bramley

1981

Archives of Andrology

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The levels of extracellular glutamic-oxalacetic transaminase (GOT: EC 2.6.1. I ) activity have been measured in cell-free supernatants of human semen and of semen + cryoprotectant mixtures both before freezing by either cryostorage or quench-freezing regimes, and after subsequent thawing. The mean level of GOT activity in seminal plasma was 293 ( 2 15 sem) IUil at 37"C, this being 15 times the level in normal blood plasma. Seminal GOT is positively correlated (p

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

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Glutamic-Oxalacetic Transaminase Leakage from Human Spermatozoa as an Indicator of Cryodamage

Bramley

1981

Archives of Andrology

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“…2.6.1 .l) and alanine aminotransferase (ALT; E.C. 2.6.1 -2) in human semen, done by Povoa and Villela (1960), Gregoire et al (1961) and Searcy et al (1962) evidenced the elevated activity of these enzymes, principally AST. (Povoa -1964;Joel and Herzberg -1965;Pumpianski and Sharon -1965;Gregoire and Moran -1973).…”

Section: Introductionmentioning

confidence: 95%

Human Semen Aspartate Aminotransferase and Alanine Aminotransferase Activity in Male Fertility Studies

Buitrago¹,

Diez²,

Battaner³

2009

Andrologia

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Summary The enzymatic activities of aminotransferases AST and ALT have been measured in the semen of a group of 98 subjects undergoing fertility studies, correlated with spermatic density and motility. An AST/ALT ratio was obtained for all patients. The results indicate that there exists a correlation between both enzymes' activities and spermatic density and motility. In two cases of excretory azoospermia, an elevated AST/ALT relation was obtained, while in two cases of secretory azoospermia a low AST/ALT ratio was seen. Aminotransferase values can thus be considered a useful piece of data in the evaluation of seminal quality, and the AST/ALT ratio used more specifically in the discrimination of the various types of azoospermia. Die Aktivität der Aspartat‐Amino‐Transferase und Alanin‐Amino‐Transferase im menschlichen Sperma bei männlichen Fertilitätsuntersuchungen Zusammenfassung Die Enzymaktivitäten der Aminotransferasen AST und ALT, die im Sperma von 98 Personen gemessen wurden, die sich einer Fertilitätsuntersuchung unterzogen, korrelierten mit der Spermatozoendichte und der Beweglichkeit. Eine Korrelation AST/ALT fand sich bei allen Patienten. In zwei Fällen mit exkretorischer Azoospermie fand sich eine hohe AST/ALT‐Relation, während in zwei Fällen mit sekretorischer Azoospermie eine niedrige AST/ALT‐Relation nachweisbar war. Die Aminotransferasewerte stellen somit ein wertvolles Teilergebnis bei der Bewertung der Spermaqualität dar, und die AST/ALT‐Korrelation erlaubt eine spezifischere Unterscheidung der verschiedenen Typen von Azoospermie.

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“…MacLeod and W röblewsld (10) investigated the lactic dehydrogenase of hum an semen and observed th a t the ac tivity of this enzyme was particularly high in seminal plasma. Very recently, Povoa and Villela (11) reported that the glutamicoxalacetic transam inase levels of the seminal plasm a of m an were approxim ately 20 times as great as in blood serum.…”

mentioning

confidence: 99%