Transcervical artificial insemination of Australian Merino ewes with frozen-thawed semen

Windsor, D.P.; Széll, A.; Buschbeck, C.; Edward, A.Y.; Milton, John; Buckrell, B.C.

doi:10.1016/0093-691x(94)90671-5

Cited by 45 publications

(29 citation statements)

References 19 publications

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“…In the separate New Zealand study of Smith et al (1995 ; Table 1), complete penetration of the cervix, using the Guelph inseminating pipette, was also much lower than that achieved by Buckrell et al (1994); however, conception rates for those ewes that were completely penetrated were similar to those achieved by Buckrell et al (1994) using laparoscopy. The same is true for the observations of Windsor et al (1994). Thus, not surprisingly, Smith et al (1995) concluded that research should concentrate on ways of increasing the incidence and ease of cervical penetration.…”

Section: Transcervical Inseminationmentioning

confidence: 94%

“…In the Halbert et al (1990a) study, complete cervical penetration was achieved in 49 of 90 ewes (54%) and 40 of the 49 ewes (82%) lambed. In the much larger Buckrell et al (1994) study, the corresponding percentages for 2060 ewes were 88% and 33%, with significant effects of the time of year (breeding v. non-breeding season) and interval from previous lambing, Table 1, on lambing outcome. Complete penetration rates increased from 76% for the first 500 ewes inseminated in the study to 98% for the last 500 and the corresponding mean times to achieve complete penetration decreased from 8.6 to 3.6 min, reflecting the importance of operator experience.…”

Section: Transcervical Inseminationmentioning

confidence: 99%

“…The observations of Halbert et al (1990a), Buckrell et al (1994), Windsor et al (1994) and Husein et al (1998) imply that there is no adverse effect, and with no accompanying information on fertility it is not known whether the damage to the cervical epithelial lining observed by Campbell et al (1996) has a detrimental effect on lambing rate. The lower lambing rates observed for transcervical than laparoscopic insemination by McKelvey (1999) and included in Table 1, and the associated trauma to the cervix, imply an adverse effect.…”

Section: Transcervical Inseminationmentioning

confidence: 99%

“…Much of the optimism in this view, at that time, was based on the improvement in complete cervical penetration provided by the Guelph equipment and insemination technique (see Table 1; Halbert et al, 1990a;Buckrell et al, 1994) and by the knowledge that complete penetration of the cervix at insemination increased the numbers and the quality of spermatozoa reaching the oviducts (Fukui and Roberts, 1977). In the Halbert et al (1990a) study, complete cervical penetration was achieved in 49 of 90 ewes (54%) and 40 of the 49 ewes (82%) lambed.…”

Section: Transcervical Inseminationmentioning

confidence: 99%

“…In the Windsor et al (1994) study, the combined lower success rate in traversing the cervix and in establishing pregnancy, compared with the Buckrell et al (1994) study, resulted in overall lambing rates of less than 1 in 4. The technique was therefore unable to compete with the success of the already well-established laparoscopic intrauterine technique in Australia.…”

Section: Transcervical Inseminationmentioning

confidence: 99%

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Traversing the ovine cervix – a challenge for cryopreserved semen and creative science

Robinson

McKelvey²,

King

et al. 2011

Animal

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This review brings together research findings on cervical relaxation in the ewe and its pharmacological stimulation for enhancement of the penetration needed for transcervical insemination and embryo transfer. On the basis that the success of artificial insemination is the percentage of ewes lambing, a review is made of recent research aimed at understanding and minimising the sub-lethal effects of freezing and thawing on the viability of spermatozoa, their membrane integrity and their ability to migrate through cervical mucus, as these characteristics have a major influence on fertility, particularly when semen is deposited, artificially, in the os cervix. Milestones of achievement are given for transcervical intrauterine insemination, embryo recovery and transfer and the birth of lambs of pre-determined sex, firstly following intracytoplasmic sperm injection, then laparoscopic intrauterine insemination using highly diluted flow-cytometrically sorted fresh semen and subsequently by os cervix insemination using sexed semen that had been frozen and thawed. Diversity of research endeavour (applied, cellular, molecular), research discipline (anatomy, histology, immunology, endocrinology) and research focus (cell, tissue, organ, whole animal) is embraced within the review as each has significant contributions to make in advancing recent scientific findings from the laboratory into robust on-farm transcervical insemination and embryo transfer techniques.Keywords: ovine cervix, semen cryopreservation, artificial insemination, embryo recovery and transfer ImplicationsIn bringing together the results of recent research on the cryopreservation of ram semen and the relaxation of the ewe cervix, this review provides a basis for identifying opportunities for improving the efficiency of cervical insemination and transcervical embryo recovery and transfer in sheep genetic improvement programmes.

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Section: Transcervical Inseminationmentioning

confidence: 94%

Section: Transcervical Inseminationmentioning

confidence: 99%

Section: Transcervical Inseminationmentioning

confidence: 99%

Section: Transcervical Inseminationmentioning

confidence: 99%

Section: Transcervical Inseminationmentioning

confidence: 99%

See 3 more Smart Citations

Traversing the ovine cervix – a challenge for cryopreserved semen and creative science

Robinson

McKelvey²,

King

et al. 2011

Animal

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Fertility and Its Relationship to Motility Characteristics of Spermatozoa in Ewes After Cervical, Transcervical, and Intrauterine Insemination With Frozen‐Thawed Ram Semen

SÁNCHEZ‐PARTIDA,

WINDSOR,

EPPLESTON

et al. 1999

Journal of Andrology

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The fertility of ewes after artificial insemination and the relationship between fertility and motility characteristics assessed by a computerized motility analysis system were examined with ram semen frozen in diluents reported to improve postthaw motility. The percentages of motile and progressive spermatozoa were better when frozen in proline—or glycine betaine‐containing or HEPES‐based, rather than Trisbased, diluents (P < 0.01). The fertility of spermatozoa frozen in diluents containing proline or glycine betaine was slightly reduced, whereas when both compatible solutes were present, the reduction was more pronounced, in comparison with semen frozen in Tris‐ or HEPES‐based diluents (9.5 versus 71.1 and 66.6%; P < 0.01). Fertility of frozen—thawed spermatozoa was higher after laparoscopic insemination than after cervical or transcervical insemination (P < 0.01). Similarly, higher fertility was obtained after cervical insemination with fresh than with frozen‐thawed semen (32.4 versus 11.3%; P < 0.01). Furthermore, loss of embryos was lower after laparoscopic insemination of ewes with semen frozen in a Tris diluent than with semen frozen in proline diluents, in glycine betaine diluents, or in proline‐plus‐glycine betaine diluents (0.0 versus 26.0, 38.5, and 60.0%; P < 0.001). A wide variation in the postthaw percentage of motile (31.6–59.7%) and progressive (22.6–43.1%) spermatozoa and in the fertility of spermatozoa from individual rams was also observed after laparoscopic (29.2–59.7%) or cervical insemination (8.7–30.5%). Postthaw motility results from immediately after thawing and fertility results from experiments where intrauterine insemination was performed with semen frozen in proline‐ or glycine betaine—containing or HEPES‐ or Tris‐based diluents were pooled and subjected to a pairwise correlation procedure. The correlation analysis showed relationships between some of the motility characteristics (P < 0.01), but there were no relationships between the motility characteristics and fertility.

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Factors influencing the success of transcervical insemination in Merino ewes

Windsor¹

1995

Theriogenology

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Transcervical artificial insemination of Australian Merino ewes with frozen-thawed semen

Cited by 45 publications

References 19 publications

Traversing the ovine cervix – a challenge for cryopreserved semen and creative science

Traversing the ovine cervix – a challenge for cryopreserved semen and creative science

Fertility and Its Relationship to Motility Characteristics of Spermatozoa in Ewes After Cervical, Transcervical, and Intrauterine Insemination With Frozen‐Thawed Ram Semen

Factors influencing the success of transcervical insemination in Merino ewes

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