Transcript abundance of resistance- and susceptibility-related genes in a barley breeding pedigree with partial resistance to the bird cherry-oat aphid (Rhopalosiphum padi L.)

Mehrabi, Sara; Åhman, Inger; Jonsson, Lisbeth

doi:10.1007/s10681-014-1093-5

Cited by 16 publications

(32 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These genotypes are related to three of the four genotypes in the study by Delp et al (2009). The study showed that the contig1636_at was present in all genotypes, as determined by genomic PCR, but constitutively expressed only in nine genotypes, seven of which are susceptible and two resistant to R. padi (Mehrabi et al 2014). In the present study, we aim at obtaining further knowledge about especially the putative susceptibility-related sequences corresponding to contig1636_at and contig1639_at.…”

Section: Introductionmentioning

confidence: 83%

“…RNA was extracted using NucleoSpin Ò RNA Plant kit from Macherey-Nagel (Düren, Germany) according to the manufacturer's protocol and stored at -80°C. Quality control of RNA, measuring of RNA concentration and cDNA synthesis were all carried out as in Mehrabi et al (2014).…”

Section: Aphid Rearingmentioning

confidence: 99%

“…The procedures, conditions and calculations for RT-qPCR were the same as in Mehrabi et al (2014). Because of the large sample size, only the leaves from three replicates per time point and treatment were included.…”

Section: Rt-qpcrmentioning

confidence: 99%

“…The primer sequences used in PCR are shown in Supplementary Table 1. The conditions and protocols were as described in Mehrabi et al (2014) except for the PCR product purification that was carried out using Gene JET PCR Purification Kit provided by Thermo Scientific (Lithuania) according to the manufacturer's protocol. Sequencing was performed by Eurofins MWG Operon (Ebersberg, Germany).…”

Section: Isolation and Sequencing Of Primer Productsmentioning

confidence: 99%

“…The transcript levels were measured 48 h after infestation with R. padi. In a more recent study, we investigated the constitutive expression of putative resistance-related genes in 23 different barley genotypes with varying resistance to R. padi (Mehrabi et al 2014). These genotypes are related to three of the four genotypes in the study by Delp et al (2009).…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

The constitutive expression and induction of three β-1,3-glucanases by bird cherry-oat aphid in relation to aphid resistance in 15 barley breeding lines

Mehrabi

Åhman

Jonsson

2016

Arthropod-Plant Interactions

Self Cite

View full text Add to dashboard Cite

In this study, 15 closely related barley genotypes were analyzed for the abundance of three b-1,3-glucanase transcripts immediately before and during infestation by the bird cherry-oat aphid (Rhopalosiphum padi L.). The barley lines are doubled haploid lines in backcross (BC) generations BC1 and BC2 from a cross between cultivar Lina and a wild barley accession. Previously, they have been characterized as susceptible (S) or resistant (R) to R. padi based on their ability to support nymphal growth. Here we also tested whether resistance was manifested as reduced aphid settling on the plants. Indeed, aphid numbers were lower on R than on S lines in all cases where there were significant differences between R and S lines. The choice of b-1,3-glucanase sequences is based on earlier results comparing two S and two R genotypes, suggesting that at least two of the three studied sequences are susceptibility factors. The comparisons of transcript abundance in plants with aphids showed for two of the b-1,3-glucanase sequences that there were several cases where an S genotype had significantly higher abundance than an R genotype, and in no case did an R line have significantly higher abundance than an S line. Thus, there was some support for the idea that b-1,3-glucanase sequences are susceptibility factors in the interaction between barley and R. padi.

show abstract

Section: Introductionmentioning

confidence: 83%

Section: Aphid Rearingmentioning

confidence: 99%

Section: Rt-qpcrmentioning

confidence: 99%

Section: Isolation and Sequencing Of Primer Productsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

The constitutive expression and induction of three β-1,3-glucanases by bird cherry-oat aphid in relation to aphid resistance in 15 barley breeding lines

Mehrabi

Åhman

Jonsson

2016

Arthropod-Plant Interactions

Self Cite

View full text Add to dashboard Cite

show abstract

A Comprehensive Analysis of Wheat Resistance to Rhopalosiphum padi (Hemiptera: Aphididae) in Brazilian Wheat Cultivars

Correa

Maciel

Bücker-Neto

et al. 2020

Journal of Economic Entomology

View full text Add to dashboard Cite

Rhopalosiphum padi L. is one of the predominant aphids affecting wheat crops worldwide. Therefore, the identification of resistant genotypes and the understanding of molecular response mechanisms involved in wheat resistance to this aphid may contribute to the development of new breeding strategies. In this study, we evaluated the resistance of 15 wheat cultivars to R. padi and performed morpho-histological and gene expression analyses of two wheat cultivars (BRS Timbaúva, resistant and Embrapa 16, susceptible) challenged and unchallenged by R. padi. The main findings of our work are as follows: 1) most Brazilian wheat cultivars recently released are resistant to R. padi; 2) Green leaf volatiles are probably involved in the resistance of the BRS Timbaúva cultivar to the aphid; 3) trichomes were more abundant and larger in the resistant cultivar; 4) the internal morphology did not show differences between cultivars; 5) the lipoxygenase-encoding gene was downregulated in the susceptible cultivar and basal expression remained level in the resistant cultivar; and 6) the expression of resistance-related proteins was induced in the resistant but not in the susceptible cultivar. Lipoxygenase is the first enzyme in the octadecanoic pathway, a well-known route for the synthesis of signaling molecules involved in the activation of plant defense. The overall analyses suggest that the key steps in BRS Timbaúva resistance to R. padi may be presence or absence of green leaf volatiles decreasing the aphid preference and the action of nonglandular trichome as a physical barrier, which allows continuous lipoxygenase-encoding gene expression.

show abstract

Drought stress increases the expression of barley defence genes with negative consequences for infesting cereal aphids

Leybourne

Valentine

Binnie

et al. 2022

Journal of Experimental Botany

View full text Add to dashboard Cite

Crops are exposed to myriad abiotic and biotic stressors with negative consequences. Two stressors that are expected to increase under climate change are drought and infestation with herbivorous insects, including important aphid species. Expanding our understanding of the impact drought has on the plant-aphid relationship will become increasingly important under future climate scenarios. Here we use a previously characterised plant-aphid system comprising a susceptible variety of barley, a wild relative of barley with partial-aphid resistance, and the bird cherry-oat aphid to examine the drought-plant-aphid relationship. We show that drought has a negative effect on plant physiology and aphid fitness and provide evidence to suggest that plant resistance influences aphid responses to drought stress. Furthermore, we show that the expression of thionin genes, plant defensive compounds that contribute to aphid resistance, increase in susceptible plants exposed to drought stress but remain at constant levels in the partially-resistant plant, suggesting they play an important role in determining the success of aphid populations. This study highlights the role of plant defensive processes in mediating the interactions between the environment, plants, and herbivorous insects.

show abstract

Transcript abundance of resistance- and susceptibility-related genes in a barley breeding pedigree with partial resistance to the bird cherry-oat aphid (Rhopalosiphum padi L.)

Cited by 16 publications

References 52 publications

The constitutive expression and induction of three β-1,3-glucanases by bird cherry-oat aphid in relation to aphid resistance in 15 barley breeding lines

The constitutive expression and induction of three β-1,3-glucanases by bird cherry-oat aphid in relation to aphid resistance in 15 barley breeding lines

A Comprehensive Analysis of Wheat Resistance to Rhopalosiphum padi (Hemiptera: Aphididae) in Brazilian Wheat Cultivars

Drought stress increases the expression of barley defence genes with negative consequences for infesting cereal aphids

Contact Info

Product

Resources

About