1993
DOI: 10.1016/0378-1097(93)90168-2
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Transcript mapping of the rubredoxin gene from Clostridium pasteurianum

Abstract: The transcripts of the rubredoxin gene from C. pasteurianum have been shown to have a size of approx. 230 bases by Northern blotting techniques. The transcription start has been located within 41 bases upstream of the initiator codon. The data demonstrate that the rubredoxin gene is monocistronic. Two of the three open reading frames occurring upstream of the rubredoxin gene are adjacent and appear to encode a thioredoxin (or glutaredoxin) reductase and a thioredoxin (or glutaredoxin).

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Cited by 3 publications
(5 citation statements)
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“…Chae, B. M. Flores, S. G. Rhee, D. L. Diedrich, and B. E. Torian, manuscript submitted). Furthermore, although most bacterial homologues to the S. typhimurium AhpR system have an identical arrangement of open reading frames corresponding to ahpC and full-length ahpF, Clostridium pasteurianum gene organization is quite different and appears to include genes encoding a smaller (TrR-like) flavoprotein, a glutaredoxin-like protein, and an AhpC homologue (Figure 7; Mathieu & Meyer, 1993;Mathieu et al, 1992). Thus, in the one case where the structural gene related to ahpC is oriented downstream of an ahpF homologue (truncated), an additional open reading frame appears to encode an additional redox protein.…”
Section: Discussionmentioning
confidence: 99%
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“…Chae, B. M. Flores, S. G. Rhee, D. L. Diedrich, and B. E. Torian, manuscript submitted). Furthermore, although most bacterial homologues to the S. typhimurium AhpR system have an identical arrangement of open reading frames corresponding to ahpC and full-length ahpF, Clostridium pasteurianum gene organization is quite different and appears to include genes encoding a smaller (TrR-like) flavoprotein, a glutaredoxin-like protein, and an AhpC homologue (Figure 7; Mathieu & Meyer, 1993;Mathieu et al, 1992). Thus, in the one case where the structural gene related to ahpC is oriented downstream of an ahpF homologue (truncated), an additional open reading frame appears to encode an additional redox protein.…”
Section: Discussionmentioning
confidence: 99%
“…Thus, in the one case where the structural gene related to ahpC is oriented downstream of an ahpF homologue (truncated), an additional open reading frame appears to encode an additional redox protein. In a Mathieu & Meyer, 1993;Mathieu et al, 1992). Partially deduced sequences are indicated by broken lines at one end, and Orf designations refer to open reading frames which were not originally identified as known structural genes.…”
Section: Discussionmentioning
confidence: 99%
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“…Eubacterium acidaminophilum and Clostridium pasteuranium (Cohen et at.. 1993;Lubbers and Andreesen. 1993;Mathieu and Meyer, 1993 The discovery of a singie gene in M. teprae that encodes both TR and Tnc (TR/Trx) raises a number of questions regarding its biological function. The gene for TR/Trx encodes a 49 kDa protein containing 458 amino acids.…”
Section: Discussionmentioning
confidence: 99%
“…The metal-substituted Rd referred to above have all been prepared by removal of the iron atom from the native protein and subsequent incorporation of the heterometal into the apoprotein. Recently, the Clostridium pasteurianum Rd gene has been expressed in Escherichia coli (23)(24)(25)(26), where Znsubstituted Rd (ZnRd) is produced along with the ironcontaining protein (FeRd; refs. 24 and 27).…”
mentioning
confidence: 99%