Transcript profile distinguishes variability in human myogenic progenitor cell expansion capacity

Riddle, Emily; Bender, Erica; Thalacker‐Mercer, Anna

doi:10.1152/physiolgenomics.00041.2018

Cited by 8 publications

(8 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The last decade has been an exciting period for the study of the biology of skeletal muscle stem cells and tissue regeneration and the development of novel human in vitro cell models can contribute to the identification of new mechanisms that control myogenesis [14][15][16][17][18][19][20]. These human cell cultures appear more suitable for predictive screening strategies when compared to rodent cell lines, such as C2C12 or rat L6 myoblasts [21,22].…”

Section: Discussionmentioning

confidence: 99%

Characterization of Skeletal Muscle Endocrine Control in an In Vitro Model of Myogenesis

et al. 2020

View full text Add to dashboard Cite

Skeletal muscle has remarkable regenerative abilities regulated by a highly orchestrated process involving the activation of cellular and molecular responses, which are dependent on satellite cells. These cells maintain the stem cell population and provide numerous myogenic cells that proliferate, differentiate, fuse and lead to new myofiber formation for a functional contractile tissue. We have isolated and characterized satellite cells obtained from human biopsies and established an in vitro model of myogenesis, evaluating muscle regeneration, monitoring the dynamic increases of the specific myogenic regulatory factors and the final formation of multinucleated myofibers. As the skeletal muscle is an endocrine tissue able of producing many substances that can act on distant organs, and it can be physiologically modulated by a variety of hormones, we embarked in a project of characterization of muscle cell endocrinology machinery. The expression of a large array of hormone receptors was quantified during the process of myogenesis. The results obtained showed a significant and generalized increase of all the tested hormone receptors along the process of differentiation of human cultured cells from myoblasts to myocytes. Interestingly, also the production of the myokine irisin increased in a parallel manner. These findings point to the human cultured myoblasts as an ideal model to characterize the skeletal muscle endocrine machinery and its hormonal regulation.

show abstract

Section: Discussionmentioning

confidence: 99%

Characterization of Skeletal Muscle Endocrine Control in an In Vitro Model of Myogenesis

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Young (21–40 years) and old adults (65–80 years) were recruited from the Ithaca, New York and Boone, North Carolina areas (participant characteristics in Supplementary Table S1) in a manner previously published (Riddle et al, 2018a,b). The Cornell University and Appalachian State University Institutional Review Boards approved the protocols.…”

Section: Methodsmentioning

confidence: 99%

Peptide YY (PYY) Is Expressed in Human Skeletal Muscle Tissue and Expanding Human Muscle Progenitor Cells

et al. 2019

Self Cite

View full text Add to dashboard Cite

Peptide YY (PYY) is considered a gut peptide with roles in post-prandial appetite and glucose regulation. Circulating PYY protein levels increase during aerobic exercise. Furthermore, people who have greater increases in muscle progenitor cells (hMPCs), the adult stem cell population responsible for skeletal muscle (SkM) repair, after resistance training have higher PYY transcript levels in SkM prior to training. Currently, examination of PYY expression patterns in SkM and/or hMPCs is lacking. Our objective was to identify the expression patterns of PYY in SkM and hMPCs. PYY and the associated Y receptors were analyzed in SkM biopsy tissue and cultured hMPCs from young and old human participants. Additional experiments to assess the role and regulation of PYY in hMPCs were performed. In SkM, PYY and one of the three Y receptors (Y1r) were detectable, but expression patterns were not affected by age. In expanding hMPCs, PYY and all three Y receptor (Y1r, Y2r, and Y5r) proteins were expressed in a temporal fashion with young hMPCs having greater levels of Y receptors at various time points. Exogenous PYY did not affect hMPC population expansion. hMPC PYY levels increased following the metabolic stimulus, 5-Aminoimidazole-4-carboxamide ribonucleotide (AICAR), but were not affected by the inflammatory stimulus, tumor necrosis factor alpha (TNFα). In conclusion, PYY and Y receptor expression are not impacted by age in SkM tissue but are reduced in old vs. young expanding hMPCs. Furthermore, endogenous PYY production is stimulated by low energy states and thus may be integral for skeletal muscle and hMPC responses to metabolic stimuli.

show abstract

“…Noise in gene expression has been shown to have a significant impact on the design and function of genetic circuits in unicellular organisms (Elowitz et al, 2002;Eldar & Elowitz, 2010). It has also been observed in multiple pathways in mammalian cells (Yin et al, 2009;Mantsoki et al, 2016;Riddle et al, 2018), in Drosophila cells (Pare et al, 2009) and between individuals in Drosophila (Lin et al, 2016). However, gene expression variability has mostly been analysed for a few individual genes in plants at a single-cell resolution (Angel et al, 2015;Araujo et al, 2017;Meyer et al, 2017;Gould et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Widespread inter‐individual gene expression variability in Arabidopsis thaliana

Cortijo

Aydın

Ahnert

et al. 2019

Molecular Systems Biology

View full text Add to dashboard Cite

A fundamental question in biology is how gene expression is regulated to give rise to a phenotype. However, transcriptional variability is rarely considered although it could influence the relationship between genotype and phenotype. It is known in unicellular organisms that gene expression is often noisy rather than uniform, and this has been proposed to be beneficial when environmental conditions are unpredictable. However, little is known about inter‐individual transcriptional variability in multicellular organisms. Using transcriptomic approaches, we analysed gene expression variability between individual Arabidopsis thaliana plants growing in identical conditions over a 24‐h time course. We identified hundreds of genes that exhibit high inter‐individual variability and found that many are involved in environmental responses, with different classes of genes variable between the day and night. We also identified factors that might facilitate gene expression variability, such as gene length, the number of transcription factors regulating the genes and the chromatin environment. These results shed new light on the impact of transcriptional variability in gene expression regulation in plants.

show abstract

Transcript profile distinguishes variability in human myogenic progenitor cell expansion capacity

Cited by 8 publications

References 50 publications

Characterization of Skeletal Muscle Endocrine Control in an In Vitro Model of Myogenesis

Characterization of Skeletal Muscle Endocrine Control in an In Vitro Model of Myogenesis

Peptide YY (PYY) Is Expressed in Human Skeletal Muscle Tissue and Expanding Human Muscle Progenitor Cells

Widespread inter‐individual gene expression variability in Arabidopsis thaliana

Contact Info

Product

Resources

About