Transcript responses in leaves of ozone-treated beech saplings seasons at an outdoor free air model fumigation site over two growing seasons

Olbrich, Maren; Gerstner, Elke; Welzl, Gerhard; Winkler, Jana Barbro; Ernst, D.

doi:10.1007/s11104-009-0129-4

Cited by 26 publications

(18 citation statements)

References 68 publications

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“…In recent decades, the effects of ozone have been studied at the physiological, biochemical and molecular biological levels [17]. Numerous transcripts, up-regulated in leaves by ozone, belong to the category “disease and defence”, which also includes pathogenesis-related (PR) protein transcripts [17], [19]. The exposure of rye cultivars and Lolium perenne to elevated ozone concentrations during plant growth increased the allergen content in their pollen [20], [21], whereas in different cultivars of Lolium , no significant differences in group 5 allergen were evident between control plants and plants grown in ozone concentrations up to 140 ppb [22].…”

Section: Introductionmentioning

confidence: 99%

Molecular and Immunological Characterization of Ragweed (Ambrosia artemisiifolia L.) Pollen after Exposure of the Plants to Elevated Ozone over a Whole Growing Season

et al. 2013

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Climate change and air pollution, including ozone is known to affect plants and might also influence the ragweed pollen, known to carry strong allergens. We compared the transcriptome of ragweed pollen produced under ambient and elevated ozone by 454-sequencing. An enzyme-linked immunosorbent assay (ELISA) was carried out for the major ragweed allergen Amb a 1. Pollen surface was examined by scanning electron microscopy and attenuated total reflectance–Fourier transform infrared spectroscopy (ATR-FTIR), and phenolics were analysed by high-performance liquid chromatography. Elevated ozone had no influence on the pollen size, shape, surface structure or amount of phenolics. ATR-FTIR indicated increased pectin-like material in the exine. Transcriptomic analyses showed changes in expressed-sequence tags (ESTs), including allergens. However, ELISA indicated no significantly increased amounts of Amb a 1 under elevated ozone concentrations. The data highlight a direct influence of ozone on the exine components and transcript level of allergens. As the total protein amount of Amb a 1 was not altered, a direct correlation to an increased risk to human health could not be derived. Additional, the 454-sequencing contributes to the identification of stress-related transcripts in mature pollen that could be grouped into distinct gene ontology terms.

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Section: Introductionmentioning

confidence: 99%

Molecular and Immunological Characterization of Ragweed (Ambrosia artemisiifolia L.) Pollen after Exposure of the Plants to Elevated Ozone over a Whole Growing Season

et al. 2013

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“…This impairment of Rubisco is accompanied by a loss of the mRNA coding for the small (rbcS) and large (rbcL) subunits of the enzyme. For example, declines in rbcS mRNA were measured in beech saplings in a free air O 3 exposure system (Olbrich et al 2009). Effects of O 3 on proteins involved in carbon assimilation have also been observed, as reductions in quantities of the small and large subunit (rbcL) of Rubisco and Rubisco activase were measured in soybean plants exposed to 120 ppb O 3 for 3 days (Ahsan et al 2010).…”

Section: Physiological Effectsmentioning

confidence: 99%

Plant-Mediated Ecosystem Effects of Tropospheric Ozone

2014

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“…O 3 enters the cell through the cell wall and plasma membrane, where it is rapidly converted into different ROS, which are highly toxic and induce changes in proteins, and cause oxidative DNA lesions (Mishra et al, 2013). O 3 also induces the up-regulation of antioxidant defense systems in plants (Olbrich et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Detection of protein and DNA damage induced by elevated carbon dioxide and ozone in Triticum aestivum L. using biomarker and comet assay

Abdelhaliem¹,

Al-Huqai²

2016

Genet. Mol. Res.

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ABSTRACT. This study was designed to compare the genetic effects of elevated carbon dioxide (CO 2 ) and ozone (O 3 ), alone or in combination, under irrigated and non-irrigated conditions on proteins and DNA of wheat (Triticum aestivum L.). Sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE), isozymes, random amplified polymorphic DNA (RAPD), and comet assays were used. SDS-PAGE analysis revealed distinctive polymorphisms (100%) based on the number of polypeptide bands (169) with molecular weights ranging from 300.0 to 24.00 kDa, band intensity, appearance, and loss of bands when compared with control samples. Six isozymes, malate dehydrogenase, amylase, leucine-aminopeptidase, esterase, peroxidase, and catalase, generated 100% polymorphism values based on zymogram number, relative front, and optical intensities. RAPD revealed 276 DNA bands with a distinctive polymorphism value of 92.31% based on the number of amplified DNA products, ranging from 45 to 1100 bp, and band intensity. In the comet assay, the highest extent of nuclear DNA damage was observed as tail length 8.70 µm, tailed DNA 8.01%, and tail moment unit 34.18 in non-irrigated O 3 -treated wheat nuclei. These results show that O 3 -treatment alone induced high levels of oxidative protein and DNA damage in wheat plant, especially in a non-irrigation system. Interestingly, CO 2 in combination with O 3 could ameliorate the negative impact of O 3 -oxidative stress. This study shows that protein and DNA biomarkers, and the comet assay, could be used for the reliable estimation of genotoxicity following the exposure of economic crop plants to air pollutants.

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Transcript responses in leaves of ozone-treated beech saplings seasons at an outdoor free air model fumigation site over two growing seasons

Abstract: The still most abundant air pollutant, tropospheric ozone, leads to severe oxidative stress in plants.

Cited by 26 publications

References 68 publications

Molecular and Immunological Characterization of Ragweed (Ambrosia artemisiifolia L.) Pollen after Exposure of the Plants to Elevated Ozone over a Whole Growing Season

Molecular and Immunological Characterization of Ragweed (Ambrosia artemisiifolia L.) Pollen after Exposure of the Plants to Elevated Ozone over a Whole Growing Season

Plant-Mediated Ecosystem Effects of Tropospheric Ozone

Detection of protein and DNA damage induced by elevated carbon dioxide and ozone in Triticum aestivum L. using biomarker and comet assay

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