Transcription factor AP-2 gamma/Krüppel-like factor 10 axis is involved in miR-3656-related dysfunction of endothelial cells in hypertension

Abstract

Background: Dysfunction of endothelial cells links to microvascular rarefaction, reflecting the pathogenesis of hypertension. Our previous studies found that miR-3656 reduces nitric oxide generation and von Willebrand factor (vWF) cleavage, thereby retarding blood flow and potentially increasing blood pressure. In this paper, we investigated mechanism of transcription regulation contributing to miR-3656-damaged endothelial cells in hypertension. Methods:The effects of miR-3656 on function of endothelial cells were analyzed on the basis of proliferation, migration, tube formation, and apoptosis. The mRNA level and protein level of genes were examined using quantitative real-time polymerase chain reaction (qRT-PCR) and western blot. Dual-luciferase reporter assay was performed to confirm the binding between miR-3656 and 3' untranslated region (UTR) of transcription factor AP-2 gamma (TFAP2C). The binding between TFAP2C and the promoter region of Krppel-like factor 10 (KLF10) was confirmed by chromatin immunoprecipitation-qPCR assay.Results: miR-3656 impaired the cell proliferation, migration, tube formation, and apoptosis of endothelial cells. miR-3656 inhibited the expression of TFAP2C by directly targeting 3'UTR of TFAP2C; moreover, miR-3656induced injury of endothelial cells was rescued by TFAP2C overexpression. Furthermore, downregulated TFAP2C decreased KLF10 expression by binding to KLF10 promoter region, and upregulated KLF10 reversed the effects of silencing TFAP2C on endothelial cells. These inhibitory processes led to interference of miR-3656 to KLF10promoted function of endothelial cells. Conclusion:TFAP2C/KLF10 axis is involved in miR-3656related dysfunction of endothelial cells in hypertension. The 3'UTR of TFAP2C and KLF10 promoter region are the hubs of the TFAP2C/KLF10 axis.

Identification of candidate biomarkers and pathways associated with multiple sclerosis using bioinformatics and next generation sequencing data analysis

“…LTF (lactotransferrin) [362], RHOB (ras homolog family member B) [396], CDH1 [377], CFTR (CF transmembrane conductance regulator) [380], hsa-mir-146a-5p [636], hsa-mir-195-5p [637], BRCA1 [638], ELK4 [639] and FOXO3 [640] might offer useful information for treating Alzheimer’s Disease. LTF (lactotransferrin) [541], CFTR (CF transmembrane conductance regulator) [551], hsa-mir-195-5p [627], BRCA1 [641], NFKB1 [642], TFAP2C [643], FOXO3 [644] and NRF1 [645] are associated with poor prognosis in patients with hypertension. Increasing evidence has convincingly demonstrated that altered expression of LTF (lactotransferrin) [580], hsa-mir-146a-5p [646], hsa-mir-195-5p [637], HNF4A [647], NFKB1 [648], NR2F1 [649], FOXO3 [650] and NRF1 [651] are a prognosis factors in Parkinson’s disease.…”