Transcription factor FfMYB15 regulates the expression of cellulase gene FfCEL6B during mycelial growth of Flammulina filiformis

Liu, Zongqi; Deng, Bing; Yuan, Hui; Zhang, Benfeng; Liu, Jingyu; Meng, Junlong; Chang, Mingchang

doi:10.1186/s12934-022-01932-z

Cited by 6 publications

(2 citation statements)

References 62 publications

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“…In fungi, AfMybA, MYT1, and MYT2 have been found to be involved in growth and development [32][33][34]. Among macro-fungi, F. velutiper and Ganoderma lucidum MYB TFs were found to be involved in growth and development [35,36]. In the context of P. ostreatus MYB gene family identification, there has been speculation that MYB might play a role in growth and development [26].…”

Section: Discussionmentioning

confidence: 99%

Function of Transcription Factors PoMYB12, PoMYB15, and PoMYB20 in Heat Stress and Growth of Pleurotus ostreatus

Yuan,

Liu,

Guo

et al. 2023

IJMS

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MYB transcription factors (TFs) have been extensively studied in plant abiotic stress responses and growth and development. However, the role of MYB TFs in the heat stress response and growth and development of Pleurotus ostreatus remains unclear. To investigate the function of PoMYB12, PoMYB15, and PoMYB20 TFs in P. ostreatus, mutant strains of PoMYB12, PoMYB15, and PoMYB20 were generated using RNA interference (RNAi) and overexpression (OE) techniques. The results indicated that the mycelia of OE-PoMYB12, OE-PoMYB20, and RNAi-PoMYB15 mutant strains exhibited positive effects under heat stress at 32 °C, 36 °C, and 40 °C. Compared to wild-type strains, the OE-PoMYB12, OE-PoMYB20, and RNAi-PoMYB15 mutant strains promoted the growth and development of P. ostreatus. These mutant strains also facilitated the recovery of growth and development of P. ostreatus after 24 h of 36 °C heat stress. In conclusion, the expression of PoMYB12 and PoMYB20 supports the mycelium’s response to heat stress and enhances the growth and development of P. ostreatus, whereas PoMYB15 produces the opposite effect.

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Section: Discussionmentioning

confidence: 99%

Function of Transcription Factors PoMYB12, PoMYB15, and PoMYB20 in Heat Stress and Growth of Pleurotus ostreatus

Yuan,

Liu,

Guo

et al. 2023

IJMS

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“…The content of corn cob in the cultivation material of H. marmoreus in this study was as high as 47%, which was the main source of cellulose, and makes cellulose account for an important proportion in the cultivation material. Therefore, the level of the enzyme activity of mycelia to degrade cellulose becomes the key to whether the strain of H. marmoreus can effectively utilize the culture substrate [ 16 ].…”

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confidence: 99%

Preliminary study on the anti-CO2 stress and growth ability of hypsizygus marmoreus mutant strain HY68

Liu,

Ma,

Chen

et al. 2023

BMC Microbiol

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Background A high concentration of CO2 will stagnate the development of the newly formed primordia of Hypsizygus marmoreus, hinder the development of the mushroom cap, thereby inhibiting the normal differentiation of the fruiting body. Moreover, in the previous experiment, our research group obtained the mutant strain HY68 of H. marmoreus, which can maintain normal fruiting under the condition of high concentration of CO2. Our study aimed to evaluate the CO2 tolerance ability of the mutant strain HY68, in comparison with the starting strain HY61 and the control strain HY62. We analyzed the mycelial growth of these strains under various conditions, including different temperatures, pH levels, carbon sources, and nitrogen sources, and measured the activity of the cellulose enzyme. Additionally, we identified and predicted β-glucosidase-related genes in HY68 and analyzed their gene and protein structures. Results Our results indicate that HY68 showed superior CO2 tolerance compared to the other strains tested, with an optimal growth temperature of 25 °C and pH of 7, and maltose and beef paste as the ideal carbon and nitrogen sources, respectively. Enzyme activity assays revealed a positive correlation between β-glucosidase activity and CO2 tolerance, with Gene14147 identified as the most closely related gene to this activity. Inbred strains of HY68 showed trait segregation for CO2 tolerance. Conclusions Both HY68 and its self-bred offspring could tolerate CO2 stress. The fruiting period of the strains resistant to CO2 stress was shorter than that of the strains not tolerant to CO2 stress. The activity of β-GC and the ability to tolerate CO2 were more closely related to the growth efficiency of fruiting bodies. This study lays the foundation for understanding how CO2 regulates the growth of edible fungi, which is conducive to the innovation of edible fungus breeding methods. The application of the new strain HY68 is beneficial to the research of energy-saving production in factory cultivation.

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Gene Silencing via RNA Interference in Cryptococcus

Bose

2024

Methods in Molecular Biology

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Transcription factor FfMYB15 regulates the expression of cellulase gene FfCEL6B during mycelial growth of Flammulina filiformis

Cited by 6 publications

References 62 publications

Function of Transcription Factors PoMYB12, PoMYB15, and PoMYB20 in Heat Stress and Growth of Pleurotus ostreatus

Function of Transcription Factors PoMYB12, PoMYB15, and PoMYB20 in Heat Stress and Growth of Pleurotus ostreatus

Preliminary study on the anti-CO2 stress and growth ability of hypsizygus marmoreus mutant strain HY68

Gene Silencing via RNA Interference in Cryptococcus

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