Transcription factor Fli-1 positively regulates lipopolysaccharide-induced interleukin-27 production in macrophages

Gao, Peng; Yuan, Ming; Ma, Xianwei; Jiang, Wei; Zhu, Lingxi; Wen, Mingyue; Xu, Jing; Liu, Qiuyan; An, Huazhang

doi:10.1016/j.molimm.2016.02.006

Cited by 10 publications

(7 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…FLI1 is a regulator involved in cell growth and differentiation of hematopoietic cells [55,56]. The protein level of FLI1 is increased in macrophages after LPS stimulation; it is involved in IL-27 production [57] and is an activator of CXCL2 (MIP-2α) in an additive manner with the p65 (RelA) subunit of NF-κB under LPS stimulation [58]. FLI1 is acetylated by p300/CBP-associated shows GO related to microtubule-based processes, Cluster 2 (score 5.57) is associated with transcription, and Cluster 3 (score 5.18) is related to organelle organization.…”

Section: Discussionmentioning

confidence: 99%

Resveratrol decreases the expression of genes involved in inflammation through transcriptional regulation

Pinheiro

Oliveira

Coutinho

et al. 2019

Free Radical Biology and Medicine

View full text Add to dashboard Cite

Oxidative stress generated during inflammation is associated with a wide range of pathologies. Resveratrol (RESV) displays anti-inflammatory and antioxidant activities, being a candidate for the development of adjuvant therapies for several inflammatory diseases. Despite this potential, the cellular responses induced by RESV are not well known. In this work, transcriptomic analysis was performed following lipopolysaccharide (LPS) stimulation of monocyte cultures in the presence of RESV. Induction of an inflammatory response was observed after LPS treatment and the addition of RESV led to decreases in expression of the inflammatory mediators, tumor necrosis factor-alpha (TNF-α), interleukin-8 (IL-8), and monocyte chemoattractant protein-1 (MCP-1), without cytotoxicity. RNA sequencing revealed 823 upregulated and 2098 downregulated genes (cutoff ≥2.0 or ≤−2.0) after RESV treatment. Gene ontology analysis showed that the upregulated genes were associated with metabolic processes and the cell cycle, consistent with normal cell growth and differentiation under an inflammatory stimulus. The downregulated genes were associated with inflammatory responses, gene expression, and protein modification. The prediction of master regulators using the iRegulon tool showed nuclear respiratory factor 1 (NRF1) and GA-binding protein alpha subunit (GABPA) as the main regulators of the downregulated genes. Using immunoprecipitation and protein expression assays, we observed that RESV was able to decrease protein acetylation patterns, such as acetylated apurinic/apyrimidinic endonuclease-1/reduction-oxidation factor 1 (APE1/Ref-1), and increase histone methylation. In addition, reductions in p65 (nuclear factor-kappa B (NF-κB) subunit) and lysine-specific histone demethylase-1 (LSD1) expression were observed. In conclusion, our data indicate that treatment with RESV caused significant changes in protein acetylation and methylation patterns, suggesting the induction of deacetylase and reduction of demethylase activities that mainly affect regulatory cascades mediated by NF-кB and Janus kinase/signal transducers and activators of transcription (JAK/STAT) signaling. NRF1 and GABPA seem to be the main regulators of the transcriptional profile observed after RESV treatment.

show abstract

Section: Discussionmentioning

confidence: 99%

Resveratrol decreases the expression of genes involved in inflammation through transcriptional regulation

Pinheiro

Oliveira

Coutinho

et al. 2019

Free Radical Biology and Medicine

View full text Add to dashboard Cite

show abstract

“…expression of several important cytokines including monocyte chemoattractant protein-1 (MCP-1), interleukin (IL)-6, and granulocyte colony-stimulating factor (G-CSF) [22,23,[27][28][29][30][31]. A recent study further suggested that (1) Fli-1 is a key regulator of inflammation in ECs [21] and (2) Fli-1 deficiency promotes proliferation and cell survival of ECs [32].…”

mentioning

confidence: 99%

Fli-1 Governs Pericyte Dysfunction in a Murine Model of Sepsis

Zhou

Goodwin

et al. 2018

The Journal of Infectious Diseases

View full text Add to dashboard Cite

show abstract

“…Prevention profile genes were enriched for distinct motifs from ETS (SpiB, PU.1) family (Figs 3J and S4E). ETS TFs are known to interact extensively with other TFs [75] and play a role in cytokine gene expression [76][77][78][79] and macrophage differentiation [39].…”

Section: Plos Pathogensmentioning

confidence: 99%

Yersinia remodels epigenetic histone modifications in human macrophages

et al. 2021

View full text Add to dashboard Cite

Various pathogens systematically reprogram gene expression in macrophages, but the underlying mechanisms are largely unknown. We investigated whether the enteropathogen Yersinia enterocolitica alters chromatin states to reprogram gene expression in primary human macrophages. Genome-wide chromatin immunoprecipitation (ChIP) seq analyses showed that pathogen-associated molecular patterns (PAMPs) induced up- or down-regulation of histone modifications (HMod) at approximately 14500 loci in promoters and enhancers. Effectors of Y. enterocolitica reorganized about half of these dynamic HMod, with the effector YopP being responsible for about half of these modulatory activities. The reorganized HMod were associated with genes involved in immune response and metabolism. Remarkably, the altered HMod also associated with 61% of all 534 known Rho GTPase pathway genes, revealing a new level in Rho GTPase regulation and a new aspect of bacterial pathogenicity. Changes in HMod were associated to varying degrees with corresponding gene expression, e. g. depending on chromatin localization and cooperation of the HMod. In summary, infection with Y. enterocolitica remodels HMod in human macrophages to modulate key gene expression programs of the innate immune response.

show abstract

Transcription factor Fli-1 positively regulates lipopolysaccharide-induced interleukin-27 production in macrophages

Cited by 10 publications

References 39 publications

Resveratrol decreases the expression of genes involved in inflammation through transcriptional regulation

Resveratrol decreases the expression of genes involved in inflammation through transcriptional regulation

Fli-1 Governs Pericyte Dysfunction in a Murine Model of Sepsis

Yersinia remodels epigenetic histone modifications in human macrophages

Contact Info

Product

Resources

About