Transcription factor OsHsfC1b regulates salt tolerance and development in Oryza sativa ssp. japonica

Schmidt, Romy; Schippers, Jos H. M.; Welker, Annelie; Mieulet, Delphine; Guiderdoni, Emmanuel; Mueller‐Roeber, Bernd

doi:10.1093/aobpla/pls011

Cited by 87 publications

(54 citation statements)

References 69 publications

(92 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Subsequently, multistep PCR was performed as described previously (Warthmann et al, 2008). The artificial microRNA construct was cloned into pC5300 OE vector according to Schmidt et al (2012). For construction of SERF1 overexpression lines, the full-length CDS of SERF1 was synthesized and sequence verified by GeneArt.…”

Section: Constructs and Rice Transformationmentioning

confidence: 99%

SALT-RESPONSIVE ERF1 Regulates Reactive Oxygen Species-Dependent Signaling during the Initial Response to Salt Stress in Rice

et al. 2013

View full text Add to dashboard Cite

Early detection of salt stress is vital for plant survival and growth. Still, the molecular processes controlling early salt stress perception and signaling are not fully understood. Here, we identified SALT-RESPONSIVE ERF1 (SERF1), a rice (Oryza sativa) transcription factor (TF) gene that shows a root-specific induction upon salt and hydrogen peroxide (H 2 O 2 ) treatment. Loss of SERF1 impairs the salt-inducible expression of genes encoding members of a mitogen-activated protein kinase (MAPK) cascade and salt tolerance-mediating TFs. Furthermore, we show that SERF1-dependent genes are H 2 O 2 responsive and demonstrate that SERF1 binds to the promoters of MAPK KINASE KINASE6 (MAP3K6), MAPK5, DEHYDRATION-RESPONSIVE ELEMENT BINDING2A (DREB2A), and ZINC FINGER PROTEIN179 (ZFP179) in vitro and in vivo. SERF1 also directly induces its own gene expression. In addition, SERF1 is a phosphorylation target of MAPK5, resulting in enhanced transcriptional activity of SERF1 toward its direct target genes. In agreement, plants deficient for SERF1 are more sensitive to salt stress compared with the wild type, while constitutive overexpression of SERF1 improves salinity tolerance. We propose that SERF1 amplifies the reactive oxygen species-activated MAPK cascade signal during the initial phase of salt stress and translates the salt-induced signal into an appropriate expressional response resulting in salt tolerance.

show abstract

Section: Constructs and Rice Transformationmentioning

confidence: 99%

SALT-RESPONSIVE ERF1 Regulates Reactive Oxygen Species-Dependent Signaling during the Initial Response to Salt Stress in Rice

et al. 2013

View full text Add to dashboard Cite

show abstract

“…The functional characterization of a class C Hsf has been reported recently in Oryza sativa (Os). OsHsfC1b serves as a regulator of salt stress response and affects plant growth under non-stress conditions [31]. Moreover, previous studies have indicated that HsfA4 has a negative correlation with the levels of ascorbate peroxidase 1 (APX1) and may function as an anti-apoptotic factor in plants [32], [33], [34].…”

Section: Introductionmentioning

confidence: 99%

Ectopic Overexpression of SlHsfA3, a Heat Stress Transcription Factor from Tomato, Confers Increased Thermotolerance and Salt Hypersensitivity in Germination in Transgenic Arabidopsis

Zhang

Wang

et al. 2013

PLoS ONE

View full text Add to dashboard Cite

Plant heat stress transcription factors (Hsfs) are the critical components involved in mediating responses to various environmental stressors. However, the detailed roles of many plant Hsfs are far from fully understood. In this study, an Hsf (SlHsfA3) was isolated from the cultivated tomato (Solanum lycopersicum, Sl) and functionally characterized at the genetic and developmental levels. The nucleus-localized SlHsfA3 was basally and ubiquitously expressed in different plant organs. The expression of SlHsfA3 was induced dramatically by heat stress, moderately by high salinity, and slightly by drought, but was not induced by abscisic acid (ABA). The ectopic overexpression of SlHsfA3 conferred increased thermotolerance and late flowering phenotype to transgenic Arabidopsis plants. Moreover, SlHsfA3 played a negative role in controlling seed germination under salt stress. RNA-sequencing data demonstrated that a number of heat shock proteins (Hsps) and stress-associated genes were induced in Arabidopsis plants overexpressing SlHsfA3. A gel shift experiment and transient expression assays in Nicotiana benthamiana leaves demonstrated that SlHsfA3 directly activates the expression of SlHsp26.1-P and SlHsp21.5-ER. Taken together, our results suggest that SlHsfA3 behaves as a typical Hsf to contribute to plant thermotolerance. The late flowering and seed germination phenotypes and the RNA-seq data derived from SlHsfA3 overexpression lines lend more credence to the hypothesis that plant Hsfs participate in diverse physiological and biochemical processes related to adverse conditions.

show abstract

“…WMD computationally generates hundreds of amiRNAs for each target that are ranked based on the complementarity and hybridization energy without knowing the in vivo effi ciency. The effi cacy of amiRNAs has been tested in Arabidopsis (Schwab et al 2006 ), rice (Warthmann et al 2008 ;Schmidt et al 2012 ), Physcomitrella patens (Khraiwesh et al 2008 ), Chlamydomonas reinhardtii (Molnar et al 2009 ), and other species. The advantages of amiRNA compared to other RNA silencing approaches such as RNA interference (RNAi) and virusinduced gene silencing (VIGS) are that it has minimal off-target effects and a unique capacity for multigene silencing (Alvarez et al 2006 ;Niu et al 2006 ;Schwab et al 2006 ;Ossowski et al 2008 ).…”

Section: Artifi Cial Micrornas (Amirnas)mentioning

confidence: 99%

Engineering of Plants for the Production of Commercially Important Products: Approaches and Accomplishments

Abdel‐Ghany

Golovkin²,

Reddy

2015

Plant Biology and Biotechnology

View full text Add to dashboard Cite

For centuries humans have used plants as a source of food, fi ber, fuel, and medicine because they have the ability to synthesize a vast array of complex organic compounds using light, carbon dioxide, and water. Advances in recombinant DNA and transgenic technologies during the last several decades have opened many new avenues to further exploit plants for production of many novel products. The potential to use plants to synthesize diverse native and nonnative industrial and pharmaceutical products coupled with the depletion of fossil fuels that are the source of many commercially important products and the adverse effects of chemical synthesis of platform chemicals on the environment have renewed considerable interest in using plants for large-scale production of chemicals and valueadded compounds. To accomplish this, different genetic engineering and transformation strategies have been developed for introducing multiple genes (gene stacking), modulating their expression with regulatable promoters, and targeting the products to a specifi c compartment in the cells. Successful metabolic engineering of plants should lead to sustained production of platform chemicals, pharmaceuticals, and biopolymers. In

show abstract

Transcription factor OsHsfC1b regulates salt tolerance and development in Oryza sativa ssp. japonica

Abstract: The paper describes the functional analysis of a class C heat shock transcription factor from rice (Oryza sativa). OsHsfC1b is shown to play a role in ABA-mediated salt stress tolerance and is required for plant growth under non-stress conditions.

Cited by 87 publications

References 69 publications

SALT-RESPONSIVE ERF1 Regulates Reactive Oxygen Species-Dependent Signaling during the Initial Response to Salt Stress in Rice

SALT-RESPONSIVE ERF1 Regulates Reactive Oxygen Species-Dependent Signaling during the Initial Response to Salt Stress in Rice

Ectopic Overexpression of SlHsfA3, a Heat Stress Transcription Factor from Tomato, Confers Increased Thermotolerance and Salt Hypersensitivity in Germination in Transgenic Arabidopsis

Engineering of Plants for the Production of Commercially Important Products: Approaches and Accomplishments

Contact Info

Product

Resources

About