Transcription factor <scp>RD</scp>26 is a key regulator of metabolic reprogramming during dark‐induced senescence

Kamranfar, Iman; Gong, Xue; Tohge, Takayuki; Sedaghatmehr, Mastoureh; Fernie, Alisdair R.; Balazadeh, Salma; Mueller‐Roeber, Bernd

doi:10.1111/nph.15127

Cited by 67 publications

(52 citation statements)

References 94 publications

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“…The AtCV and AtNYC1 genes positively regulate chloroplast degradation in Arabidopsis [15,38,39]. Consistent with the observations of AtCV and AtNYC1 in Arabidopsis, the transcription levels of PGSC0003DMG400002261 (StCV) and PGSC0003DMG400030219 (StNYC1) genes were significantly up-regulated with dark treatment.…”

Section: Overexpressed Stabi5 Potato Lines Accelerate Dark-induced Lesupporting

confidence: 83%

StABI5 Involved in the Regulation of Chloroplast Development and Photosynthesis in Potato

Zhu

Feng

et al. 2020

IJMS

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Abscisic acid (ABA) insensitive 5 (ABI5)—a core transcription factor of the ABA signaling pathway—is a basic leucine zipper transcription factor that plays a key role in the regulation of seed germination and early seedling growth. ABI5 interacts with other phytohormone signals to regulate plant growth and development, and stress responses in Arabidopsis, but little is known about the functions of ABI5 in potatoes. Here, we find that StABI5 is involved in the regulation of chloroplast development and photosynthesis. Genetic analysis indicates that StABI5 overexpression transgenic potato lines accelerate dark-induced leaf yellowing and senescence. The chlorophyll contents of overexpressed StABI5 transgenic potato lines were significantly decreased in comparison to those of wild-type Desiree potatoes under dark conditions. Additionally, the RNA-sequencing (RNA-seq) analysis shows that many metabolic processes are changed in overexpressed StABI5 transgenic potatoes. Most of the genes involved in photosynthesis and carbon fixation are significantly down-regulated, especially the chlorophyll a-b binding protein, photosystem I, and photosystem II. These observations indicate that StABI5 negatively regulates chloroplast development and photosynthesis, and provides some insights into the functions of StABI5 in regard to potato growth.

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Section: Overexpressed Stabi5 Potato Lines Accelerate Dark-induced Lesupporting

confidence: 83%

StABI5 Involved in the Regulation of Chloroplast Development and Photosynthesis in Potato

Zhu

Feng

et al. 2020

IJMS

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“…SSPP functions in sustaining leaf longevity and its overexpression significantly delays leaf senescence in Arabidopsis [53]. The transcription factor RD26 (ANAC072) is a positive regulator of stress-and darkness-induced senescence [54,55]. As we recently demonstrated, an enhanced expression of RD26 triggers the accumulation of free amino acids (in particular Gln and Asn), enhances the accumulation of tricarboxylic acid (TCA) cycle intermediates and represses the accumulation of γ-aminobutyric acid (GABA; [54]).…”

Section: Annotation Of Genes Induced By Sf For Oxidative Stress Protementioning

confidence: 99%

A Biostimulant Obtained from the Seaweed Ascophyllum nodosum Protects Arabidopsis thaliana from Severe Oxidative Stress

Omidbakhshfard

Sujeeth

Gupta

et al. 2020

IJMS

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Abiotic stresses cause oxidative damage in plants. Here, we demonstrate that foliar application of an extract from the seaweed Ascophyllum nodosum, SuperFifty (SF), largely prevents paraquat (PQ)-induced oxidative stress in Arabidopsis thaliana. While PQ-stressed plants develop necrotic lesions, plants pre-treated with SF (i.e., primed plants) were unaffected by PQ. Transcriptome analysis revealed induction of reactive oxygen species (ROS) marker genes, genes involved in ROS-induced programmed cell death, and autophagy-related genes after PQ treatment. These changes did not occur in PQ-stressed plants primed with SF. In contrast, upregulation of several carbohydrate metabolism genes, growth, and hormone signaling as well as antioxidant-related genes were specific to SF-primed plants. Metabolomic analyses revealed accumulation of the stress-protective metabolite maltose and the tricarboxylic acid cycle intermediates fumarate and malate in SF-primed plants. Lipidome analysis indicated that those lipids associated with oxidative stress-induced cell death and chloroplast degradation, such as triacylglycerols (TAGs), declined upon SF priming. Our study demonstrated that SF confers tolerance to PQ-induced oxidative stress in A. thaliana, an effect achieved by modulating a range of processes at the transcriptomic, metabolic, and lipid levels.

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“…Interestingly, all the AFS3 identified proteins have been related to stress responses, in particular to biotic stress, with the exception of ENODL1 like protein At5g53870, that was shown to be promoted by drought and heat (Rizhsky et al, 2004); and only few AFS3 proteins have been previously related to leaf senescence. One of them is the amylase AMY1, that locates to the apoplast where it might degrade cell wall associated carbohydrates during Arabidopsis senescence (Doyle et al, 2007;Kamranfar et al, 2018), AMY1 expression is also induced by ABA, heat, and pathogen-related stresses (Doyle et al, 2007). Arabidopsis amy1 plants show early flowering (Jie et al, 2009).…”

Section: Proteins That Accumulate In the Apoplast Of Senescent Leavesmentioning

confidence: 99%

Physiological and Proteomic Changes in the Apoplast Accompany Leaf Senescence in Arabidopsis

et al. 2020

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The apoplast, i.e. the cellular compartment external to the plasma membrane, undergoes important changes during senescence. Apoplastic fluid volume increases quite significantly in senescing leaves, thereby diluting its contents. Its pH elevates by about 0.8 units, similar to the apoplast alkalization in response to abiotic stresses. The levels of 159 proteins decrease, whereas 24 proteins increase in relative abundance in the apoplast of senescing leaves. Around half of the apoplastic proteins of non-senescent leaves contain a N-terminal signal peptide for secretion, while all the identified senescence-associated apoplastic proteins contain the signal peptide. Several of the apoplastic proteins that accumulate during senescence also accumulate in stress responses, suggesting that the apoplast may constitute a compartment where developmental and stress-related programs overlap. Other senescence-related apoplastic proteins are involved in cell wall modifications, proteolysis, carbohydrate, ROS and amino acid metabolism, signaling, lipid transport, etc. The most abundant senescence-associated apoplastic proteins, PR2 and PR5 (e.g. pathogenesis related proteins PR2 and PR5) are related to leaf aging rather than to the chloroplast degradation program, as their levels increase only in leaves undergoing developmental senescence, but not in dark-induced senescent leaves. Changes in the apoplastic space may be relevant for signaling and molecular trafficking underlying senescence.

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Transcription factor RD26 is a key regulator of metabolic reprogramming during dark‐induced senescence

Cited by 67 publications

References 94 publications

StABI5 Involved in the Regulation of Chloroplast Development and Photosynthesis in Potato

StABI5 Involved in the Regulation of Chloroplast Development and Photosynthesis in Potato

A Biostimulant Obtained from the Seaweed Ascophyllum nodosum Protects Arabidopsis thaliana from Severe Oxidative Stress

Physiological and Proteomic Changes in the Apoplast Accompany Leaf Senescence in Arabidopsis

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