Transcription factors Sox8 and Sox10 perform non-equivalent roles during oligodendrocyte development despite functional redundancy

Stolt, C. Claus; Lommes, Petra; Friedrich, Ralf P.; Wegner, Michael

doi:10.1242/dev.01114

Cited by 188 publications

(210 citation statements)

References 36 publications

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“…Sox10 can have limited functional redundancy (Stolt et al, 2004;Maka et al, 2005;Kellerer et al, 2006) and furthermore that ectopic expression of Sox9 will induce endogenous Sox10 expression in some cell types (Cheung and Briscoe, 2003). In our present study the detection of Sox10 in pre-Sertoli cells of the male genital ridge between e11.5 to e13.5 suggests a potential involvement in sex determination and differentiation ( Fig.…”

Section: Genital Ridge Expression Of Selectedsupporting

confidence: 57%

Presumptive pre‐sertoli cells express genes involved in cell proliferation and cell signalling during a critical window in early testis differentiation

Cory

Boyer

Pilon

et al. 2007

Molecular Reproduction Devel

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In mammals, the pre-Sertoli cell of the male genital ridge is the first cell type to display sex specific differentiation and differential gene expression. The genetic cascade driving the differentiationof pre-Sertoli cells and ultimately testis formationis beginning to be unravelled, but many questions remain. A better understanding of the transcriptome of pre-Sertoli cells immediately after sex determination is essential in order to further understand this differentiationprocess. A mouse model expressing Red Fluorescent Protein (RFP) under the control of a hybrid mouse/pig SRY promoter (HybSRYp-RFP) was used to purify cells from embryonic day 12.0 (e12.0) male genital ridges. To compare the transcriptomes of HybSRYp-RFP cell populations versus age matched whole female genital ridges, RNA was extracted and used to generate molecular probes that were hybridized onto Affymetrix Mouse Genome 430 2.0 microarrays. The expression of genes considered markers for pre-Sertoli cells, including Sox9, Mis, Dhh and Fgf9 were identified within the HybSRYp-RFP expressing cell population, while markers for germ cells (Oct4, SSEA-1) and endothelial cells (Ntrk3) were not identified. In contrast, markers for ovarian somatic cell expression, including Fst and Bmp2, were identified as overexpressed within the ovarian cell population. In a general fashion, genes identified as 2.5-fold over expressed in HybSRYp-RFP expressing cells coded notably for cell signalling and extra cellular proteins. The expression of Sox10, Stc2, Fgf18, Fgf13 and Wnt6 were further characterized via whole mount in situ hybridization (WISH) on male and female genital ridges between e11.5 and e14.5. Sox10, Fgf18, Fgf13 and Stc2 gene expression was detected within the male genital ridges while Wnt6 was found diffusely within both the male and female genital ridges. These data represent the earliest comprehensive microarray expression analysis of purified presumptive pre-Sertoli cells available to date. Mol. Reprod. Dev. 74: 1491-1504, 2007

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Section: Genital Ridge Expression Of Selectedsupporting

confidence: 57%

Presumptive pre‐sertoli cells express genes involved in cell proliferation and cell signalling during a critical window in early testis differentiation

Cory

Boyer

Pilon

et al. 2007

Molecular Reproduction Devel

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“…As a consequence, Sox10 deletion leads to a simultaneous loss of Sox8 expression, whereas Sox8 deletion leaves Sox10 expression unaffected. This dependence of Sox8 on Sox10 is cell type specific because it has not been observed in oligodendroglial cells that also express Sox8 and Sox10 (Stolt et al, 2004). Furthermore, Sox10 alone is not sufficient to maintain Sox8 expression as Sox8 is down-regulated in neural crest-derived cells of the adrenal gland from 14.5 dpc onward despite the continued presence of Sox10.…”

Section: Discussionmentioning

confidence: 97%

SoxE Proteins Are Differentially Required in Mouse Adrenal Gland Development

Reiprich

Stolt

Schreiner

et al. 2008

MBoC

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Sry-box (Sox)8, Sox9, and Sox10 are all strongly expressed in the neural crest. Here, we studied the influence of these closely related transcription factors on the developing adrenal medulla as one prominent neural crest derivative. Whereas Sox9 was not expressed, both Sox8 and Sox10 occurred widely in neural crest cells migrating to the adrenal gland and in the gland itself, and they were down-regulated in cells expressing catecholaminergic traits. Sox10-deficient mice lacked an adrenal medulla. The adrenal anlage was never colonized by neural crest cells, which failed to specify properly at the dorsal aorta and died apoptotically during migration. Furthermore, mutant neural crest cells did not express Sox8. Strong adrenal phenotypes were also observed when the Sox10 dimerization domain was inactivated or when a transactivation domain in the central portion was deleted. Sox8 in contrast had only minimal influence on adrenal gland development. Phenotypic consequences became only visible in Sox8-deficient mice upon additional deletion of one Sox10 allele. Replacement of Sox10 by Sox8, however, led to significant rescue of the adrenal medulla, indicating that functional differences between the two related Sox proteins contribute less to the different adrenal phenotypes of the null mutants than dependence of Sox8 expression on Sox10.

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“…So far, high-mobility group (Sox8, Sox10), basic helix-loophelix (Olig1, Olig2), and homeodomain (Nkx6.2, Nkx2.2) factors have been linked to myelin gene enhancer activity (Awatramani et al, 1997;Lu et al, 2000;Zhou et al, 2001;Stolt et al, 2004;Denarier et al, 2005;Ghislain and Charnay, 2006;Liu et al, 2007). As enhancers from the coordinately regulated myelin gene family emerge, characterization of their element content and configuration should reveal shared components and common features of the code controlling myelin gene transcription (Farhadi et al, 2003;Denarier et al, 2005;Dionne, 2006).…”

Section: Discussionmentioning

confidence: 99%

Separate Proteolipid Protein/DM20 Enhancers Serve Different Lineages and Stages of Development

Tuason¹,

Rastikerdar²,

Kuhlmann³

et al. 2008

Journal of Neuroscience

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The gene encoding DM20 emerged in cartilaginous fish, descending from a bilaterian ancestor of the M6 proteolipid gene family. Its proteolipid protein (PLP) isoform appeared in amphibians, contains an additional 35 amino acids, and, in the mammalian CNS, is the dominant myelin protein in which it confers an essential neuroprotective function. During development, the DM20 isoform is prominent in a number of tissues, and plp/DM20 transcripts are detected in multiple progenitor populations, including those that continue to express plp/DM20 as they differentiate into myelinating oligodendrocytes. The locus also encodes isoforms with extended leader sequences that accumulate in the cell bodies of several types of neurons. Here, to locate and characterize regulatory sequences controlling the complex plp/DM20 transcription program, putative regulatory sequences, suggested by interspecies conservation, were ligated individually to a minimally promoted eGFPlacZ reporter gene. These constructs were inserted in single copy at a common site adjacent to the hypoxanthine-guanine phosphoribosyltransferase locus in embryonic stem cells and their in vivo expression programs were compared in transgenic mice. Most expressed developmental and cell-specific subprograms accommodated within the known expression phenotype of the endogenous plp/DM20 locus, thus defining multiple components of the combinatorial mechanism controlling its normal temporal and cell-specific program. Along with previously characterized nervous system enhancers, those described here should help expose the content and configuration of elements that are operational in multiple glial and neuronal lineages. The transgenic lines derived here also provide effective markers for multiple stages of glial and neuronal lineage progression.

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Transcription factors Sox8 and Sox10 perform non-equivalent roles during oligodendrocyte development despite functional redundancy

Cited by 188 publications

References 36 publications

Presumptive pre‐sertoli cells express genes involved in cell proliferation and cell signalling during a critical window in early testis differentiation

Presumptive pre‐sertoli cells express genes involved in cell proliferation and cell signalling during a critical window in early testis differentiation

SoxE Proteins Are Differentially Required in Mouse Adrenal Gland Development

Separate Proteolipid Protein/DM20 Enhancers Serve Different Lineages and Stages of Development

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