Transcription Mediated By rpoN-Dependent Promoters

Valls, Marc; Cases, Ildefonso; Lorenzo, Vı́ctor de

doi:10.1007/978-1-4419-9084-6_10

Cited by 8 publications

(5 citation statements)

References 123 publications

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“…While initially identified for its role in nitrogen assimilation, σ 54 proteins have been appropriated in different organisms to control many other physiological processes ranging from utilization of alternative carbon sources and control of detoxification systems, through assembly of motility organs, to the production of extracellular alginate (reviewed in Valls et al ., 2004). Much of the signal‐responsive expression of these systems is built into the regulatory circuits of σ 54 ‐promoters through trans ‐acting proteins and/or cis ‐acting domains that control the activities of the cognate obligatory bEBP.…”

Section: Introductionmentioning

confidence: 99%

Signal sensory systems that impact σ⁵⁴-dependent transcription

Shingler

2011

FEMS Microbiol Rev

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Alternative σ-factors of bacteria bind core RNA polymerase to program the specific promoter selectivity of the holoenzyme. Signal-responsive changes in the availability of different σ-factors redistribute the RNA polymerase among the distinct promoter classes in the genome for appropriate adaptive, developmental and survival responses. The σ(54) -factor is structurally and functionally distinct from all other σ-factors. Consequently, binding of σ(54) to RNA polymerase confers unique features on the cognate holoenzyme, which requires activation by an unusual class of mechano-transcriptional activators, whose activities are highly regulated in response to environmental cues. This review summarizes the current understanding of the mechanisms of transcriptional activation by σ(54) -RNA polymerase and highlights the impact of global regulatory factors on transcriptional efficiency from σ(54) -dependent promoters. These global factors include the DNA-bending proteins IHF and CRP, the nucleotide alarmone ppGpp, and the RNA polymerase-targeting protein DksA.

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Section: Introductionmentioning

confidence: 99%

Signal sensory systems that impact σ⁵⁴-dependent transcription

Shingler

2011

FEMS Microbiol Rev

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“…The σ 54 -factor is involved in controlling many physiological processes that are responsive to environmental cues ranging from assembly of motility organs and chemotaxis transducers, through nitrogen assimilation and the utilization of different carbon sources, to alginate biosynthesis (reviewed in Valls et al ., 2004). Hence, global regulatory factors that alter the activity or availability of σ 54 -RNA polymerase ( σ 54 -RNAP) have the potential to mediate farreaching effects on integrated bacterial responses.…”

Section: Introductionmentioning

confidence: 99%

The guanosine tetraphosphate (ppGpp) alarmone, DksA and promoter affinity for RNA polymerase in regulation of σ⁵⁴‐dependent transcription

Bernardo

Johansson

Solera

et al. 2006

Molecular Microbiology

130

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SummaryThe RNA polymerase-binding protein DksA is a cofactor required for guanosine tetraphosphate (ppGpp)-responsive control of transcription from s 70 promoters. Here we present evidence: (i) that both DksA and ppGpp are required for in vivo s 54 transcription even though they do not have any major direct effects on s 54 transcription in reconstituted in vitro transcription and s -factor competition assays, (ii) that previously defined mutations rendering the housekeeping s

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“…1A). Putative Ϫ10 and Ϫ35 elements for 70 of pseudomonads (36) were found 254 and 224 bp upstream of the start codon, respectively, whereas no characteristic pseudomonad 70 or 54 binding motifs (37) were observed 33 bp upstream of the start codon. Therefore, the regions 254 and 224 bp upstream of the start codon were identified as the transcriptional start sites for pnd.…”

Section: Resultsmentioning

confidence: 86%

Effects of Three Different Nucleoid-Associated Proteins Encoded on IncP-7 Plasmid pCAR1 on Host Pseudomonas putida KT2440

Suzuki-Minakuchi

Hirotani

Shintani

et al. 2015

Appl Environ Microbiol

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e Nucleoid-associated proteins (NAPs), which fold bacterial DNA and influence gene transcription, are considered to be global transcriptional regulators of genes on both plasmids and the host chromosome. Incompatibility P-7 group plasmid pCAR1 carries genes encoding three NAPs: H-NS family protein Pmr, NdpA-like protein Pnd, and HU-like protein Phu. In this study, the effects of single or double disruption of pmr, pnd, and phu were assessed in host Pseudomonas putida KT2440. When pmr and pnd or pmr and phu were simultaneously disrupted, both the segregational stability and the structural stability of pCAR1 were markedly decreased, suggesting that Pmr, Pnd, and Phu act as plasmid-stabilizing factors in addition to their established roles in replication and partition systems. The transfer frequency of pCAR1 was significantly decreased in these double mutants. The segregational and structural instability of pCAR1 in the double mutants was recovered by complementation of pmr, whereas no recovery of transfer deficiency was observed. Comprehensive phenotype comparisons showed that the host metabolism of carbon compounds, which was reduced by pCAR1 carriage, was restored by disruption of the NAP gene(s). Transcriptome analyses of mutants indicated that transcription of genes for energy production, conversion, inorganic ion transport, and metabolism were commonly affected; however, how their products altered the phenotypes of mutants was not clear. The findings of this study indicated that Pmr, Pnd, and Phu act synergistically to affect pCAR1 replication, maintenance, and transfer, as well as to alter the host metabolic phenotype.

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Transcription Mediated By rpoN-Dependent Promoters

Cited by 8 publications

References 123 publications

Signal sensory systems that impact σ⁵⁴-dependent transcription

Signal sensory systems that impact σ⁵⁴-dependent transcription

The guanosine tetraphosphate (ppGpp) alarmone, DksA and promoter affinity for RNA polymerase in regulation of σ⁵⁴‐dependent transcription

Effects of Three Different Nucleoid-Associated Proteins Encoded on IncP-7 Plasmid pCAR1 on Host Pseudomonas putida KT2440

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Transcription Mediated By rpoN-Dependent Promoters

Cited by 8 publications

References 123 publications

Signal sensory systems that impact σ54-dependent transcription

Signal sensory systems that impact σ54-dependent transcription

The guanosine tetraphosphate (ppGpp) alarmone, DksA and promoter affinity for RNA polymerase in regulation of σ54‐dependent transcription

Effects of Three Different Nucleoid-Associated Proteins Encoded on IncP-7 Plasmid pCAR1 on Host Pseudomonas putida KT2440

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Signal sensory systems that impact σ⁵⁴-dependent transcription

Signal sensory systems that impact σ⁵⁴-dependent transcription

The guanosine tetraphosphate (ppGpp) alarmone, DksA and promoter affinity for RNA polymerase in regulation of σ⁵⁴‐dependent transcription