Transcription of ribonucleic acid in isolated mouse myeloma nuclei

Marzluff, William F.; Murphy, Edwin C.; Huang, Ru Chih C.

doi:10.1021/bi00742a013

Cited by 206 publications

(109 citation statements)

References 8 publications

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“…Size Analysis of the RNA Synthesized in Isolated Nuclei. As previously reported (11), nuclei can synthesize RNA of a wide range in molecular weight, Analysis of the RNA synthesized in vitro by sedimentation velocity in the presence of SDS confirms the report that all molecular weight classes of RNA (4-80S) are synthesized (data not shown).…”

Section: Introductionsupporting

confidence: 88%

“…This drug therefore can be used in order to easily differentiate between ribosomal and nonribosomal RNA synthesis. A---A RNA released from nuclei incubations without cytoplasm RNA synthesis in nuclei isolated from mouse myeloma cells is partially inhibited by a-amanitin (11). Our experiments confirm these results.…”

Section: Introductionsupporting

confidence: 86%

“…Isolation of Nuclei. Nuclei were isolated by a slight modification of the procedure described by Marzluff et al (11).…”

Section: Introductionmentioning

confidence: 99%

“…Cells (8)(9)(10)(11)(12) x 105/ml) were collected by sedimentation at 60g for 10 minutes and washed three times in 30 mM Tris HCl pH 7.5, 120 mM KC1, 5 mM Mg acetate, 7 mM 2-mercaptoethanol.…”

Section: Introductionmentioning

confidence: 99%

“…Most of the eukaryotic messenger RNAs and some of the HnRNA contain poly(A) sequences at their 3' end (21) (7) (8) (9). Marzluff et al (11) have shown that nuclei isolated from mouse myeloma cells are capable of synthesizing poly(A) sequences. Our experiments have confirmed these findings.…”

Section: Introductionmentioning

confidence: 99%

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Synthesis of messenger RNA-like molecules in isolated myeloma nuclei

Mory

Getter

1977

Nucl Acids Res

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Nuclei isolated from mouse myeloma cells grown in tissue culture are capable of synthesizing RNA for prolonged periods of time. Addition of cytoplasmic extracts to the system stimulates slightly the rate and prolongs the time of synthesis.As judged by sedimentation in SDS and in formamide gradients, the size of the RNA synthesized is heterogeneous from smaller than 10S to larger than 45S, thus resembling in vivo made RNA.

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Section: Introductionsupporting

confidence: 88%

Section: Introductionsupporting

confidence: 86%

“…Isolation of Nuclei. Nuclei were isolated by a slight modification of the procedure described by Marzluff et al (11).…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Synthesis of messenger RNA-like molecules in isolated myeloma nuclei

Mory

Getter

1977

Nucl Acids Res

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Flow cytometric analysis of isolated rat liver nuclei during growth

Papa

Capitani²,

Matteucci

et al. 1987

Cytometry

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The development of hepatocyte polyploidy in rats aged up to 4 months was analyzed by flow cytometry using both scatter and fluorescent parameters to distinguish DNA diploid and DNA tetraploid populations and to discriminate between parenchymal and non‐parenchymal compartments. The precise origin of each class of nuclei was assessed in whole liver homogenate using purified hepatocytes, obtained by liver perfusion followed by separation on Percoll gradient, and identifying the peaks corresponding to parenchymal nuclei. The results indicate that preparative procedures involving homogenization of the rat liver tissue caused loss of the DNA octaploid population. Data on the relative proportion of the different DNA ploidy elements during rat liver development, which are in good agreement with those observed by cell analysis by means of microspectrophotometry, indicate the usefulness of flow cytometry as a choice method for the analysis of ploidy distribution.

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The role of transcription and messenger RNA stability in the regulation of epidermal growth factor receptor gene expression in regenerating mouse liver

1992

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The influence of partial hepatectomy on epidermal growth factor receptor gene expression was studied in mouse liver. Epidermal growth factor receptor binding and epidermal growth factor receptor messenger RNA levels in the liver showed a rapid peak 8 hr after partial hepatectomy, whereas the sham operation had no effects on these levels. The peak epidermal growth factor receptor messenger RNA level was approximately threefold higher than preoperative values. The increase in epidermal growth factor receptor messenger RNA levels occurred primarily as a consequence of an increase in the rate of transcription. Partial hepatectomy slightly increased the half-life of epidermal growth factor receptor messenger RNA in the liver from 2.8 to 3.6 hr. Treatment of partially hepatectomized mice with cycloheximide increased hepatic epidermal growth factor receptor messenger RNA levels about fivefold by prolonging the half-life of the messenger RNA to 11.2 hr, although this treatment inhibited the increase in transcription induced by partial hepatectomy. Cycloheximide also increased epidermal growth factor receptor messenger RNA levels in the liver or kidney of sham-operated mice about threefold, primarily through stabilizing epidermal growth factor receptor messenger RNA. In contrast, cycloheximide had no effects on beta-actin messenger RNA levels in the liver and kidney. These results suggest that transcription induced by partial hepatectomy requires protein synthesis and that labile proteins are involved in the regulation of the stability of epidermal growth factor receptor messenger RNA.

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Transcription of ribonucleic acid in isolated mouse myeloma nuclei

Cited by 206 publications

References 8 publications

Synthesis of messenger RNA-like molecules in isolated myeloma nuclei

Synthesis of messenger RNA-like molecules in isolated myeloma nuclei

Flow cytometric analysis of isolated rat liver nuclei during growth

The role of transcription and messenger RNA stability in the regulation of epidermal growth factor receptor gene expression in regenerating mouse liver

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