Transcription of the German cockroach densovirus BgDNV genome: Alternative processing of viral RNAs

Kapelinskaya, T. V.; Martynova, Elena; Korolev, A. L.; Schal, Coby; Муха, Д. В.

doi:10.1134/s1607672908040042

Cited by 3 publications

(2 citation statements)

References 14 publications

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“…AdDNV displays split VP ORFs, and its two largest structural proteins have different extensions to which no roles have yet been assigned. PfDNV (33), PcDNV (25), BgDNV (9,17), and MpDNV (31,32), which all have a split VP ORF, with the smaller ORF encoding the PLA2 motif, probably have similar expression strategies (27). For PfDNV, a large number of donor and acceptor splicing sites have also been identified in the VP ORFs.…”

Section: Resultsmentioning

confidence: 99%

The Acheta domesticus Densovirus, Isolated from the European House Cricket, Has Evolved an Expression Strategy Unique among Parvoviruses

Liu

Jousset

et al. 2011

J Virol

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Insect parvoviruses (densoviruses) belong to the Densovirinae subfamily of the Parvoviridae and are small, isometric, nonenveloped viruses (diameter, ϳ25 nm) that contain a linear single-stranded DNA of 4 to 6 kb (2, 3, 27). These viruses can be subdivided into two large groups, those with ambisense genomes and those with monosense genomes. Like vertebrate parvoviruses, all densoviruses have a genomic DNA with hairpins at both ends, often (but not necessarily for all genera) as inverted terminal repeats (ITRs). All densoviruses with ambisense genomes package both complementary strands in equimolecular ratios as single strands in separate capsids (27). The nonstructural (NS) gene cassette is found in the 5Ј half of one genome strand, and the structural protein (VP) gene cassette is found in the 5Ј half of the complementary strand. By convention, the genome is oriented so that the NS cassette is found in the left half. Expression strategies of densoviruses often involve (alternative) splicing and leaky scanning translation mechanisms (28). So far, the near-atomic structures of three densoviruses, Penaeus stylirostris densovirus (PstDNV), Bombyx mori densovirus 1 (BmDNV-1), and Galleria mellonella densovirus (GmDNV), have been solved (10,11,21). The capsid of densoviruses consists of 60 subunits (Tϭ1) of identical proteins that may contain N-terminal extensions not involved in capsid formation but that confer additional functions to the capsid. One of these functions is a phospholipase A2 (PLA2) activity that is required for genome delivery during infection (34). Densoviruses are usually highly pathogenic for their natural hosts (5).The monosense densoviruses have been classified into 3 uniform genera, i.e., Iteravirus, with a 5.0-kb genome, 0.25-kb ITRs, and a PLA2 motif in VP; Brevidensovirus, with a 4.0-kb genome, no ITRs but terminal hairpins, and no PLA2 motif; and Hepanvirus, with a single member, hepatopancreatic parvovirus, with a 6.3-kb genome also lacking a PLA2 motif and ITRs but with 0.2-kb terminal hairpins (23,27). In contrast, the ambisense densoviruses have been classified into one uniform genus, Densovirus, with a 6-kb genome and 0.55-kb ITRs, and a second genus, Pefudensovirus, with only Periplaneta fuliginosa densovirus (PfDNV) as a member, with a 5.5-kb genome, 0.2-kb ITRs, and a split VP gene cassette (2, 26). Ribosome frameshifts have been proposed to connect its VP open reading frames (ORFs) (33). So far, all ambisense densoviruses have an N-terminal PLA2 motif in their largest VP. Some sequenced ambisense densoviruses, e.g., Myzus persicae densovirus (MpDNV) (32), Blattella germanica densovirus (BgDNV) (18), and Planococcus citri densovirus (PcDNV) (25), are as yet unclassified. The ambisense virus Culex pipiens densovirus (CpDNV) has a different genome organization for both the NS

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Section: Resultsmentioning

confidence: 99%

The Acheta domesticus Densovirus, Isolated from the European House Cricket, Has Evolved an Expression Strategy Unique among Parvoviruses

Liu

Jousset

et al. 2011

J Virol

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“…These viruses fall within the Narnaviridae , Phasmaviridae , Xinmoviridae , Phenuiviridae , Rhabdoviridae , Orthomyxoviridae , and chuviruses, along with a single orthomyxo-like virus and a single nairo-related virus. Members of the Parvoviridae (ssDNA) have also been found in cockroaches [ 23 , 24 ].…”

Section: Introductionmentioning

confidence: 99%

Unmapped RNA Virus Diversity in Termites and Their Symbionts

Lay

Shī

Buček

et al. 2020

Viruses

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Despite their ecological importance, nothing is known about the diversity and abundance of RNA viruses in termites (Termitoidae). We used a metatranscriptomics approach to determine the RNA virome structure of 50 diverse species of termite that differ in both phylogenetic position and colony composition. From these samples, we identified 67 novel RNA viruses, characterized their genomes, quantified their abundance and inferred their evolutionary history. These viruses were found within or similar to those from the Togaviridae, Iflaviridae, Polycipiviridae, Flaviviridae, Leviviridae, Narnaviridae, Mitoviridae, Lispivirdae, Phasmaviridae, Picobirnaviridae and Partitiviridae. However, all viruses identified were novel and divergent, exhibiting only 20% to 45% amino acid identity to previously identified viruses. Our analysis suggested that 17 of the viruses identified were termite-infecting, with the remainder likely associated with the termite microbiome or diet. Unclassified sobemo-like and bunya-like viruses dominated termite viromes, while most of the phylogenetic diversity was provided by the picobirna- and mitovirus-like viruses. Of note was the identification of a novel flavi-like virus most closely related to those found in marine vertebrates and invertebrates. Notably, the sampling procedure had the strongest association with virome composition, with greater RNA virome diversity in libraries prepared from whole termite bodies than those that only sampled heads.

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Diaphorina citri densovirus is a persistently infecting virus with a hybrid genome organization and unique transcription strategy

Nigg

Falk²

2020

Journal of General Virology

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Transcription of the German cockroach densovirus BgDNV genome: Alternative processing of viral RNAs

Cited by 3 publications

References 14 publications

The Acheta domesticus Densovirus, Isolated from the European House Cricket, Has Evolved an Expression Strategy Unique among Parvoviruses

The Acheta domesticus Densovirus, Isolated from the European House Cricket, Has Evolved an Expression Strategy Unique among Parvoviruses

Unmapped RNA Virus Diversity in Termites and Their Symbionts

Diaphorina citri densovirus is a persistently infecting virus with a hybrid genome organization and unique transcription strategy

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