Transcription of the <i>Arabidopsis CPD</i> gene, encoding a steroidogenic cytochrome P450, is negatively controlled by brassinosteroids

Mathur, Jaideep; Molnár, Gergely; Fujioka, Shozo; Takatsuto, Suguru; Sakurai, Akira; Yokota, Takao; Adam, Günter; Voigt, B.; Nagy, Ferenc; Maas, Christoph; Schell, Jeff; Koncz, Csaba; Szekeres, Miklós

doi:10.1046/j.1365-313x.1998.00158.x

Cited by 216 publications

(175 citation statements)

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“…In transgenic Arabidopsis, BR biosynthesis could be increased by overexpression of DWF4/CYP90B1, one of the ratelimiting enzymes (Choe et al, 2001), and parallel changes in the amounts of CPD:b-glucuronidase and native CPD transcripts revealed that the mRNA level of CPD is controlled primarily at the level of transcription (Mathur et al, 1998). Accordingly, reporter fusions with promoters of BR-biosynthetic genes, such as those of CPD, CYP85A1, and CYP85A2, could be used successfully to determine developmental and temporal expression patterns, as well as active sites of BR synthesis (Mathur et al, 1998;Castle et al, 2005). Whereas the crucial role and relatively high transcription level of CPD make its promoter ideal for in vivo expression assays using LUC reporter, such studies require faithful correspondence between the activities of endogenous CPD and the LUC reporter.…”

Section: Discussionmentioning

confidence: 99%

“…The CPD:LUC1 reporter construct was generated by cloning the 2968 to 25 segment (relative to the translation start) of the CPD promoter (Mathur et al, 1998) into the T-DNA-based binary vector pPCV812 (Koncz et al, 1994), in which the uidA (b-glucuronidase) part had been replaced by the firefly (Photynus pyralis) LUC-derived LUC1 (Promega). The CYP85A2 promoter (2971 to 25) was cloned in the same vector, as was described by Castle et al (2005).…”

Section: Generation Of Transgenic Plantsmentioning

confidence: 99%

“…More precise localization of transcriptional activity could be achieved in transgenic plants carrying reporter genes fused to the CPD, CYP85A1, or CYP85A2 promoters (Mathur et al, 1998;Castle et al, 2005), proving the efficiency of reporter-based expression analysis. Because CPD is more actively transcribed than other BR-biosynthetic P450 genes , we generated transgenic Arabidopsis lines expressing firefly (Photynus pyralis) luciferase (LUC) under control of the CPD promoter in order to allow in vivo monitoring of temporal changes in promoter activity by luminescence imaging.…”

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confidence: 94%

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Diurnal Regulation of the Brassinosteroid-Biosynthetic CPD Gene in Arabidopsis

et al. 2006

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Plant steroid hormones, brassinosteroids (BRs), are essential for normal photomorphogenesis. However, the mechanism by which light controls physiological functions via BRs is not well understood. Using transgenic plants carrying promoter-luciferase reporter gene fusions, we show that in Arabidopsis (Arabidopsis thaliana) the BR-biosynthetic CPD and CYP85A2 genes are under diurnal regulation. The complex diurnal expression profile of CPD is determined by dual, light-dependent, and circadian control. The severely decreased expression level of CPD in phytochrome-deficient background and the red light-specific induction in wildtype plants suggest that light regulation of CPD is primarily mediated by phytochrome signaling. The diurnal rhythmicity of CPD expression is maintained in brassinosteroid insensitive 1 transgenic seedlings, indicating that its transcriptional control is independent of hormonal feedback regulation. Diurnal changes in the expression of CPD and CYP85A2 are accompanied by changes of the endogenous BR content during the day, leading to brassinolide accumulation at the middle of the light phase. We also show that CPD expression is repressed in extended darkness in a BR feedback-dependent manner. In the dark the level of the bioactive hormone did not increase; therefore, our data strongly suggest that light also influences the sensitivity of plants to BRs.

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Section: Discussionmentioning

confidence: 99%

Section: Generation Of Transgenic Plantsmentioning

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Diurnal Regulation of the Brassinosteroid-Biosynthetic CPD Gene in Arabidopsis

et al. 2006

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“…The CPD gene is expressed in the cotyledons and young leaves of developing seedlings and is down-regulated by BL (34). Because BL is undetectable in rosettes of bas1-D phyB-4, one would expect higher expression of CPD.…”

Section: Discussionmentioning

confidence: 99%

BAS1 : A gene regulating brassinosteroid levels and light responsiveness in Arabidopsis

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Nguyen

Malancharuvil

et al. 1999

Proc. Natl. Acad. Sci. U.S.A.

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The Arabidopsis bas1-D mutation suppresses the long hypocotyl phenotype caused by mutations in the photoreceptor phytochrome B (phyB). The adult phenotype of bas1-D phyB-4 double mutants mimics that of brassinosteroid biosynthetic and response mutants. bas1-D phyB-4 has reduced levels of brassinosteroids and accumulates 26-hydroxybrassinolide in feeding experiments. The basis for the mutant phenotype is the enhanced expression of a cytochrome P450 (CYP72B1). bas1-D suppresses a phyB-null allele, but not a phyA-null mutation, and partially suppresses a cryptochrome-null mutation. Seedlings with reduced BAS1 expression are hyperresponsive to brassinosteroids in a light-dependent manner and display reduced sensitivity to light under a variety of conditions. Thus, BAS1 represents one of the control points between multiple photoreceptor systems and brassinosteroid signal transduction.

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“…Although, the existence of BRs in vascular cells of pine tree has been reported (Kim et al, 1990), it is still unknown if BRs are biosynthesized in the vascular tissues themselves. Mathur and others indicated that a chimeric CPD promoter-uid gene is strongly expressed in palisade cells, suggesting that a key enzyme for synthesizing BRs is expressed in mesophyll cells (Mathur et al, 1998). Although they did not refer to the expression of the chimeric gene in the vasculature, some pictures appear to show a preferential expression of the gene in vascular networks in leaves.…”

Section: Brs Rapidly Increase Before Entry Into the Final Stagementioning

confidence: 99%

Brassinosteroid Levels Increase Drastically Prior to Morphogenesis of Tracheary Elements

et al. 2001

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As the first step toward understanding the involvement of endogenous brassinosteroids (BRs) in cytodifferentiation, we analyzed biosynthetic activities of BRs in zinnia (Zinnia elegans L. cv Canary Bird) cells differentiating into tracheary elements. The results of feeding experiments suggested that both the early and late C6-oxidation pathways occur during tracheary element differentiation. Gas chromatography-mass spectrometry analysis revealed that five BRs, castasterone, typhasterol, 6-deoxocastasterone, 6-deoxotyphasterol, and 6-deoxoteasterone, actually existed in cultured zinnia cells and culture medium. Quantification of endogenous BRs in each stage of tracheary element differentiation by gas chromatography-mass spectrometry exhibited that they increased dramatically prior to the morphogenesis, which was consistent with the idea that BRs are necessary for the initiation of the final stage of tracheary element differentiation. Moreover, the proportion of each BR in culture medium was quite different from that in cells, suggesting that specific BRs are selectively secreted into medium and may function outside the cells.

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Transcription of the Arabidopsis CPD gene, encoding a steroidogenic cytochrome P450, is negatively controlled by brassinosteroids

Cited by 216 publications

References 41 publications

Diurnal Regulation of the Brassinosteroid-Biosynthetic CPD Gene in Arabidopsis

Diurnal Regulation of the Brassinosteroid-Biosynthetic CPD Gene in Arabidopsis

BAS1 : A gene regulating brassinosteroid levels and light responsiveness in Arabidopsis

Brassinosteroid Levels Increase Drastically Prior to Morphogenesis of Tracheary Elements

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