Transcriptional Analysis of <i>serk1</i> and <i>serk3</i> Coreceptor Mutants

Esse, G. Wilma van; Hove, Colette A. ten; Guzzonato, Francesco; Esse, H. Peter van; Boekschoten, Mark V.; Ridder, Lars; Vervoort, Jacques; Vries, S.C. de

doi:10.1104/pp.16.01478

Cited by 3 publications

(1 citation statement)

References 103 publications

(128 reference statements)

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“…Moreover, exogenous application of brassinosteroids to Arabidopsis seedlings reduced the levels of glucosinolates, suggesting that brassinosteroids negatively affect defense compounds 36 . Our observation that StSERK3A/B- RNAi plants contain higher levels of defense metabolites is also in accordance with the analysis of Arabidopsis serk1-3serk3-2 roots, which had higher levels of aliphatic glucosinolates as well as 4-methoxy-3-indol-3-ylmethyl glucosinolate 37 . The latter compound is a substrate of the atypical myrosinase PEN2 38,39 , which is required for penetration resistance against nonhost pathogens, such as P. infestans 40 .…”

Section: Discussionsupporting

confidence: 90%

Early Pep-13-induced immune responses are SERK3A/B-dependent in potato

Nietzschmann

Gorzolka

Smolka

et al. 2019

Sci Rep

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Potato plants treated with the pathogen-associated molecular pattern Pep-13 mount salicylic acid- and jasmonic acid-dependent defense responses, leading to enhanced resistance against Phytophthora infestans, the causal agent of late blight disease. Recognition of Pep-13 is assumed to occur by binding to a yet unknown plasma membrane-localized receptor kinase. The potato genes annotated to encode the co-receptor BAK1, StSERK3A and StSERK3B, are activated in response to Pep-13 treatment. Transgenic RNAi-potato plants with reduced expression of both SERK3A and SERK3B were generated. In response to Pep-13 treatment, the formation of reactive oxygen species and MAP kinase activation, observed in wild type plants, is highly reduced in StSERK3A/B-RNAi plants, suggesting that StSERK3A/B are required for perception of Pep-13 in potato. In contrast, defense gene expression is induced by Pep-13 in both control and StSERK3A/B-depleted plants. Altered morphology of StSERK3A/B-RNAi plants correlates with major shifts in metabolism, as determined by untargeted metabolite profiling. Enhanced levels of hydroxycinnamic acid amides, typical phytoalexins of potato, in StSERK3A/B-RNAi plants are accompanied by significantly decreased levels of flavonoids and steroidal glycoalkaloids. Thus, altered metabolism in StSERK3A/B-RNAi plants correlates with the ability of StSERK3A/B-depleted plants to mount defense, despite highly decreased early immune responses.

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Section: Discussionsupporting

confidence: 90%

Early Pep-13-induced immune responses are SERK3A/B-dependent in potato

Nietzschmann

Gorzolka

Smolka

et al. 2019

Sci Rep

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Identifying receptor-like kinases that enable Caulobacter RHG1 to promote plant growth in Arabidopsis thaliana

Lampens,

Vandecasteele,

Braem

et al. 2024

Journal of Plant Interactions

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BRI1 Signaling in the Root is Mediated through the SERK1 and SERK3 Co-receptors

Bui¹

2020

JTAS

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Brassinosteroid (BR) is a class of polyhydroxysteroids plant hormones known to regulate shoot and root growth. Genetic and molecular analyses demonstrate that receptor kinase BRI1 protein acts as a perceiver for BR. One of the characteristics of bri1 mutant’s phenotypes is the complete BR insensitivity in the root. Biochemical evidences of the BRI1 protein complex indicates that somatic embryogenesis receptor kinase 1 (SERK1) and SERK3 participate in the BR pathway in Arabidopsis root. While only serk3 mutants show partial reduction to BR sensitivity, serk1 presents a normal BR penetration phenotype compared to the wild type. Interestingly, the double mutant serk1serk3 displays more, but not full resistance to BR in root length assay. In this study, we aimed to enhance the BR insensitivity of the double mutant serk1serk3 by crossing serk1 mutant allele with a strong serk3 and bri1 mutant alleles. In our study, by generating serk1-3serk3-2 double mutants, a complete insensitivity to BR that phenocopied bri1-301 mutant was recorded. However, we were unable to increase BR resistance in the root of serk1-3serk3-2 double mutant by crossing with bri1 mutant allele in the triple mutant serk1-3serk3-2bri1. As a result, all the BRI1 signaling in the root was mediated through the SERK1 and SERK3 co-receptors. Additionally, we established that based on conventional BR assays, the At1g27190 protein was also involved in BR signaling. Preliminary data indicated that the triple mutant serk1serk3-2At1g27190 showed a dwarfed phenotype. Whether or not this dwarfed phenotype is linked to BRI1 signaling impairment needs to be further investigated.

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Transcriptional Analysis of serk1 and serk3 Coreceptor Mutants

Cited by 3 publications

References 103 publications

Early Pep-13-induced immune responses are SERK3A/B-dependent in potato

Early Pep-13-induced immune responses are SERK3A/B-dependent in potato

Identifying receptor-like kinases that enable Caulobacter RHG1 to promote plant growth in Arabidopsis thaliana

BRI1 Signaling in the Root is Mediated through the SERK1 and SERK3 Co-receptors

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