Transcriptional changes of antioxidant responses, hormone signalling and developmental processes evoked by the Brassica napus SHOOTMERISTEMLESS during in vitro embryogenesis

Elhiti, Mohamed; Yang, Cunchun; Belmonte, Mark F.; Gulden, Robert H.; Stasolla, Claudio

doi:10.1016/j.plaphy.2012.06.024

Cited by 9 publications

(9 citation statements)

References 68 publications

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“…Such approach was in agreement with the observed upregulation of genes and the increase in the activity of antioxidative enzymes-i.e. superoxide dismutase (SOD) and/ or catalase (CAT)-as well as the accumulation of low molecular weight antioxidants detected in embryogenic microspores (Vrinten et al 1999;Maraschin et al 2006;Muñoz-Amatriaín et al 2006;Jacquard et al 2009;Elhiti et al 2012;Uváčková et al 2012). Both the above-mentioned enzymes-SOD, which catalyse the dismutation of O 2•− to H 2 O 2 , and CAT, which subsequently detoxifies H 2 O 2 to O 2 and H 2 O-help maintain the redox balance and play a crucial role in cell defence against oxidative stress.…”

Section: Supplementary Informationmentioning

confidence: 99%

Triticale and barley microspore embryogenesis induction requires both reactive oxygen species generation and efficient system of antioxidative defence

Żur

Dubas

Krzewska

et al. 2021

Plant Cell Tiss Organ Cult

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The effectiveness of microspore embryogenesis (ME) is determined by a complex network of internal and environmental factors. In the present study on triticale and barley, strong positive correlation (r = 0.85) between the generation of hydrogen peroxide (H2O2) and ME effectiveness confirmed the important role of reactive oxygen species in microspore reprogramming. However, for high effectiveness of ME induction, intensive H2O2 generation had to be associated with high activity of antioxidative enzymes, superoxide dismutase and catalase. The strong seasonal effect on the physiological status of microspores revealed in the study suggests a kind of ‘biological clock’ controlling plant reproduction, crucial for microspore viability and embryogenic potential. Although the effect of various modifications of ME-inducing stress tiller pre-treatment was determined mainly by the physiological condition of microspores, at higher stress intensity positive effects induced by antioxidant molecules—reduced glutathione and its precursor, l-2-oxothiazolidine-4-carboxylic acid—were observed. High level of variation in the response to ME-inducing stress tiller pre-treatment was also revealed between the two DH lines of triticale and two cultivars of barley and among microspores isolated from subsequently developed spikes.

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Section: Supplementary Informationmentioning

confidence: 99%

Triticale and barley microspore embryogenesis induction requires both reactive oxygen species generation and efficient system of antioxidative defence

Żur

Dubas

Krzewska

et al. 2021

Plant Cell Tiss Organ Cult

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“…Several studies have indicated the possibility that ascorbic acid or glutathione application increases ME effectiveness. In the majority of cases, both compounds were supplemented to the induction or regeneration media, which resulted in enhanced ELS formation and green plant regeneration [ 12 , 13 , 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

Impact of Ascorbate—Glutathione Cycle Components on the Effectiveness of Embryogenesis Induction in Isolated Microspore Cultures of Barley and Triticale

et al. 2021

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Enhanced antioxidant defence plays an essential role in plant survival under stress conditions. However, excessive antioxidant activity sometimes suppresses the signal necessary for the initiation of the desired biological reactions. One such example is microspore embryogenesis (ME)—a process of embryo-like structure formation triggered by stress in immature male gametophytes. The study focused on the role of reactive oxygen species and antioxidant defence in triticale (x Triticosecale Wittm.) and barley (Hordeum vulgare L.) microspore reprogramming. ME was induced through various stress treatments of tillers and its effectiveness was analysed in terms of ascorbate and glutathione contents, total activity of low molecular weight antioxidants and activities of glutathione–ascorbate cycle enzymes. The most effective treatment for both species was a combination of low temperature and exogenous application of 0.3 M mannitol, with or without 0.3 mM reduced glutathione. The applied treatments induced genotype-specific defence responses. In triticale, both ascorbate and glutathione were associated with ME induction, though the role of glutathione did not seem to be related to its function as a reducing agent. In barley, effective ME was accompanied by an accumulation of ascorbate and high activity of enzymes regulating its redox status, without direct relation to glutathione content.

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“…To better understand the role of altered BnLEC1 and BnFUS3 expression during embryogenesis and the morphological abnormalities resulting from their miss-expression, we also measured the transcript abundance of 8 molecular marker genes at different stages of MDE development (late-globular, 7 day; cotyledonary, 14 day; and fully developed, 21 day). Expression of these genes was linked to proper embryonic development [14], [31].…”

Section: Resultsmentioning

confidence: 99%

“…Analysis of gene expression in developing [7,14, and 21 day] B. napus MDEs was determined by quantitative qRT-PCR [13]. Expression studies were conducted for molecular marker genes identified in previous studies [14], [31] and required for proper embryo formation.…”

Section: Methodsmentioning

confidence: 99%

Effects of altered expression of LEAFY COTYLEDON1 and FUSCA3 on microspore-derived embryogenesis of Brassica napus L.

Elahi

Duncan

Stasolla

2016

Journal of Genetic Engineering and Biotechnology

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Brassica napus (Bn) microspore-derived embryogenesis has become a model system to study basic aspects of plant development. Recognized transcription factors governing embryogenesis include: FUSCA3 (FUS3), a member of the plant-specific B3-domain family, and LEAFY COTYLEDON1 (LEC1), a member of the HAP3 subunit of the CCAAT binding factor family. The effects of altered expression of both genes were investigated during microspore-derived embryogenesis in established B. napus lines over-expressing or down-regulating BnLEC1, as well as in tilling lines where BnFUS3 was mutated. While over-expression of BnLEC1 decreases the yield of microspore-derived embryos (MDEs) without affecting their ability to regenerate plants, suppression of BnLEC1 or BnFUS3 reduced both embryo number and regeneration frequency. Embryos produced by these lines showed structural abnormalities accompanied by alterations in the expression of several embryogenesis-marker genes. Oil accumulation was also altered in the transgenic MDEs. Total oil content was increased in MDEs over-expressing BnLEC1 and decreased in those suppressing BnLEC1 or BnFUS3. Mutation of BnFUS3 also resulted in a small but significant increase in linoleic (C18:2) acid. Together this study demonstrates the crucial role of BnLEC1 and BnFUS3 during in vitro embryogenesis.

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Transcriptional changes of antioxidant responses, hormone signalling and developmental processes evoked by the Brassica napus SHOOTMERISTEMLESS during in vitro embryogenesis

Cited by 9 publications

References 68 publications

Triticale and barley microspore embryogenesis induction requires both reactive oxygen species generation and efficient system of antioxidative defence

Triticale and barley microspore embryogenesis induction requires both reactive oxygen species generation and efficient system of antioxidative defence

Impact of Ascorbate—Glutathione Cycle Components on the Effectiveness of Embryogenesis Induction in Isolated Microspore Cultures of Barley and Triticale

Effects of altered expression of LEAFY COTYLEDON1 and FUSCA3 on microspore-derived embryogenesis of Brassica napus L.

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