Transcriptional control of the phenol hydroxylase gene phe of Corynebacterium glutamicum by the AraC-type regulator PheR

Abstract

Corynebacterium glutamicum can degrade phenol by a meta-cleavage pathway, which depends on ncgl2588 (phe) of the phe operon encoding phenol hydroxylase. An additional gene, ncgl2587 (pheR), is located upstream of phe. The pheR encodes an AraC/XylR-type regulator protein with 377 amino acid residues and is transcribed in the same direction as phe. Disruption of pheR by homologous recombination resulted in the accumulation of phenol in C. glutamicum. PheR demonstrates a low type of constitutive expression where phenol induces phe expression. PheR shares 75% sequence identity with AraC-type regulator of Corynebacterium lubricantis and 37 conserved residues, characteristic of AraC family, were located. A constructed pK18mobsacB-P:lacZ transcriptional fusion plasmid was transformed into the wild-type, ΔpheR, and ΔpheR strains, and the results indicated that PheR activates the expression of phe encoding phenol hydroxylase. Electrophoretic mobility shift assay (EMSA) demonstrated a direct interaction of PheR with the phe promoter region and binding site of PheR on the P was located 109-bp upstream of phe, as indicated by foot printing analysis. Our research provides deep insight into PheR expression and its regulatory function on Phe in C. glutamicum.

Overlap of Promoter Recognition Specificity of Stress Response Sigma Factors SigD and SigH in Corynebacterium glutamicum ATCC 13032

“…Such damage to the cell wall might induce a similar stress response as lysozyme treatment. However, effects of toxic aromatic compounds, such as phenol, vanillin and ferulic acid, which are degraded by C. glutamicum (Chen et al, 2016, 2017, 2018), on σ D regulon induction have not yet been tested.…”

Abasy Atlas v2.2: The most comprehensive and up-to-date inventory of meta-curated, historical, bacterial regulatory networks, their completeness and system-level characterization

“…Four new regulators of different types have been examined in detail, exerting in total 63 new direct transcriptional interactions. Moreover, the predicted regulatory role of the AraC/XylR-type protein Cg2965 (PheR) has been confirmed by experimental data [50,51]. PheR activates the expression of the phe gene (cg2966) encoding phenol hydroxylase, allowing C. glutamicum to degrade phenol by a meta-cleavage pathway.…”

“…PheR activates the expression of the phe gene (cg2966) encoding phenol hydroxylase, allowing C. glutamicum to degrade phenol by a meta-cleavage pathway. Electrophoretic mobility shift assays (EMSAs) demonstrated a direct interaction of the purified PheR protein with the phe promoter region [51]. The MarR-type regulator CrtR (Cg0725) is encoded upstream and in divergent orientation of the carotenoid biosynthesis operon crtEcg0722crtBIYEb in C. glutamicum.…”

Effect of Separate and Combined Toxicity of Bisphenol A and Zinc on the Soil Microbiome

“…The content of zinc in dry matter above 300 mg kg −1 is considered to be toxic [ 33 ]. The phytotoxic effect of zinc may cause structural and developmental disturbances in plant cells [ 34 ].…”