Transcriptional factors, Mafs and their biological roles

Tsuchiya, Masaharu; Misaka, Ryoichi; Nitta, Kosaku; Tsuchiya, Ken

doi:10.4239/wjd.v6.i1.175

Cited by 45 publications

(50 citation statements)

References 56 publications

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“…V-maf musculoaponeurotic fibrosarcoma oncogene homologue B (MafB), belonging to basic leucine zipper transcription factor, is a widely expressed and involved in several biological events [3,4]. Initially, MafB was shown to form heterodimers with v-Maf and Fos through its zipper structure and induced transformation of chicken embryo fibroblasts in vitro [5].…”

Section: Introductionmentioning

confidence: 99%

Transcription Factor MafB Promotes Hepatocellular Carcinoma Cell Proliferation through Up-Regulation of Cyclin D1

Yu¹,

Jiang²,

Xu³

et al. 2016

Cell Physiol Biochem

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Background/Aims: MafB, a member of the Maf transcription factor family, plays a key role in the regulation of pancreatic alpha and beta cell differentiation. However, its function in the control of cancer cell proliferation remains unknown. Methods: The mRNA and protein expression levels of MafB in hepatocellular carcinoma tissues and adjacent non-tumor normal specimens were determined by real-time RT-PCR and Western blot, respectively. Report assay was performed to determine whether the regulation of Cyclin D1 by MafB is at the transcriptional level. The binding of MafB to the Cyclin D1 promoter was determined by Chromatin Immunoprecipitation (ChIP) assays. To determine the potential oncogenic effects of MafB in vivo, HepG2 cells transfected with adenovirus containing empty vector or MafB were injected subcutaneously to the skin under the front legs of the nude mice. Results: In the current study, we showed that MafB was markedly up-regulated in hepatocellular carcinoma (HCC) tissues and cells. Enforced overexpression of MafB enhanced, while its deficiency inhibited HCC cell proliferation. Mechanistically, Cyclin D1, an important regulator of cell cycle progression, was identified as a direct transcriptional target of MafB. Consistently, knockdown of Cyclin D1 largely attenuated the proliferative roles of MafB in HCC cells. Importantly, MafB overexpression significantly promoted cancer cell growth in mice. Conclusions: Collectively, our results identified a novel HCC regulatory pathway involving MafB and Cyclin D1, the dysfunction of which drives proliferative character in HCC.

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Section: Introductionmentioning

confidence: 99%

Transcription Factor MafB Promotes Hepatocellular Carcinoma Cell Proliferation through Up-Regulation of Cyclin D1

Yu¹,

Jiang²,

Xu³

et al. 2016

Cell Physiol Biochem

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“…C-MAF is a member of large MAF protein subfamily which is a subgroup of MAF family together with MAFA, MAFB, and NRL [14,15]. Similar to other Maf family proteins, mouse c-Maf has a basic region leucine zipper (bZIP) structure and forms a homodimer or heterodimer with other compatible bZIP proteins, including other large Maf proteins, c-Fos, and c-Jun [14].…”

Section: Discussionmentioning

confidence: 99%

“…Similar to other Maf family proteins, mouse c-Maf has a basic region leucine zipper (bZIP) structure and forms a homodimer or heterodimer with other compatible bZIP proteins, including other large Maf proteins, c-Fos, and c-Jun [14]. Dimerized large Maf proteins bind to DNA and act as transcriptional factors that are essential for the regulation of cell differentiation in several organs such as the brain, eye, kidney, and pancreas [14,15]. C-MAF is expressed in the proximal tubules of human [15] and mouse [16] fetal kidney.…”

Section: Discussionmentioning

confidence: 99%

“…Dimerized large Maf proteins bind to DNA and act as transcriptional factors that are essential for the regulation of cell differentiation in several organs such as the brain, eye, kidney, and pancreas [14,15]. C-MAF is expressed in the proximal tubules of human [15] and mouse [16] fetal kidney. Renal proximal tubular cells of c-Maf-knockout mice show significantly smaller cytoplasmic volume than those of wild-type mice [16].…”

Section: Discussionmentioning

confidence: 99%

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A common variant of MAF/c-MAF, transcriptional factor gene in the kidney, is associated with gout susceptibility

et al. 2017

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diagnosed as gout and 1221 male control subjects without hyperuricemia or a history of gout by TaqMan method. As a result, the major allele (C), which reportedly increases SUA levels, had a higher frequency in the gout cases (58.8%) than in the controls (55.0%). A logistic regression analysis showed a significant association between rs889472 and gout (p = 0.029, odds ratio = 1.17; 95% confidence interval 1.02-1.34). C-MAF is reported as a pivotal transcriptional factor in the development and differentiation of renal proximal tubular cells. Because urate is mainly regulated in renal proximal tubular cells, c-MAF may have an important role in urate regulation in the kidney and influence not only SUA but also gout susceptibility. Our finding shows that rs889472 of c-MAF is associated with gout susceptibility.

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“…MAFB y MAFF pertenecen a la misma familia Maf de factores de transcripción, que está implicada en el desarrollo, diferenciación, y funcionamiento de multitud de órganos, con efectos sobre el páncreas, el cartílago, tejido adiposos, mieloma, etc. (272). Unos niveles incrementados de MAFB se asocian con la gravedad de la enfermedad pulmonar obstructiva crónica, y un empeoramiento de la función pulmonar en fumadores (273), mediante la inhibición de la apoptosis de macrófagos (274).…”

Section: T>cunclassified

Efecto de la dexametasona sobre el mecanismo inflamatorio que subyace en el asma

Vadillo¹

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Que el trabajo titulado "Efecto de la dexametasona sobre el mecanismo inflamatorio que subyace en el asma", que presenta la licenciada en Biología Dña. Elena Marcos Vadillo ha sido realizado bajo nuestra dirección y reúne, a nuestro juicio, originalidad y contenidos suficientes para que sea presentado ante el Tribunal correspondiente y optar al grado de Doctora por la Universidad de Salamanca. Y para que así conste y a los efectos oportunos, expedimos el presente certificado en Salamanca a 25 de Mayo de 2016. ______________________________ ______________________________ Fdo: Prof. Dr. Ignacio J. Dávila González Fdo: Profª Dra. Catalina S. Sanz Lozano ___________________________________ Fdo: Profª Dra. Mª Asunción García Sánchez vi vii A David A mis hermanas A mis padres … por su apoyo incondicional … por quererme tanto viii ix Agradecimientos Quisiera dar las gracias a toda la gente que de una forma u otra ha hecho posible la realización de este trabajo. En primer lugar, a mis directores de tesis, los doctores Ignacio Dávila González, Catalina Sanz Lozano y Mª Asunción García Sánchez, por su profesionalidad, por su confianza, y por enseñarme cómo trabaja un buen equipo. A la doctora María Isidoro García, por su implicación y dedicación en todo momento. Por guiarme en este proyecto. A Choni y a Laura, porque, aunque no lo sepan, conocerlas me hizo mucho más fácil mi vida en Salamanca. A Laura por su eterna sonrisa, por su amabilidad, por su alegría. A Choni por todo, por enseñarme, por su tiempo, por apoyarme, por su amistad. A Iñaki por sus charlas y su locura. A Mª Paz por su cariño, por cuidarme. Los dos han sido un apoyo importante en esta tesis y en la residencia. Al Servicio de Inmunoalergia, por acogernos a todos los que pasamos por allí. A Carmen, que nos soporta a todos y a Fernando por ayudarme con los ratones. Al Servicio de Bioquímica, por enseñarme a trabajar, por los días de guardia, por los cafés en la salita. A mi tutora, Belén, y a mis compañeros residentes. A Virginia por hacer de R mayor cuando no lo era. A mis padres, por creer en mí. Por apoyarme de forma incondicional, por ser un referente en mi vida. A mis hermanas porque las quiero. A María por sus puzles en la escalera. A Ana y a Fer, porque sé que están ahí. A Daniel y Lucía, porque sin darse cuenta, hacen que se me olvide todo. A mi David, por esperarme siempre. Por comprenderme, por cuidarme, por ser mi complemento, por toda una vida.Gracias.x xi

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Transcriptional factors, Mafs and their biological roles

Cited by 45 publications

References 56 publications

Transcription Factor MafB Promotes Hepatocellular Carcinoma Cell Proliferation through Up-Regulation of Cyclin D1

Transcription Factor MafB Promotes Hepatocellular Carcinoma Cell Proliferation through Up-Regulation of Cyclin D1

A common variant of MAF/c-MAF, transcriptional factor gene in the kidney, is associated with gout susceptibility

Efecto de la dexametasona sobre el mecanismo inflamatorio que subyace en el asma

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