Transcriptional Induction of Regulatory Genes Madcam1, S1pr1, Cxcr4 and Ccr7 that Control Lymphocyte Recirculation and Homing in Terms of Experimental Gestational Diabetes Affects T-cells Distribution in Mesenteric Lymph Nodes in Offspring

Prozorova, T. M.; Kamyshny, Alexandr; Topol, I. A.

doi:10.25040/ecpb2017.01.023

Eksp. klìn. fìzìol. bìohìm.

2017

DOI: 10.25040/ecpb2017.01.023

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Transcriptional Induction of Regulatory Genes Madcam1, S1pr1, Cxcr4 and Ccr7 that Control Lymphocyte Recirculation and Homing in Terms of Experimental Gestational Diabetes Affects T-cells Distribution in Mesenteric Lymph Nodes in Offspring

T. M. Prozorova¹,

Alexandr Kamyshny²,

I. A. Topol³

Abstract: Брижові лімфатичні вузли (БЛВ) є основним місцем індукції периферійної імунологічної толерантності до різноманітних антигенів, у тому числі й панкреатичних [13], а також основним "перехідним" пунктом для пулу рециркулюючих лімфоцитів кишково-асоційованої лімфоїдної тканини (КАЛТ). Головними регуляторами хоумінгу лімфоцитів у БЛВ є адресин Madcam1 (mucosal addressin cell adhesion molecule 1)-адгезивний білок венул із високим ендотелієм лімфоїдних утворень КАЛТ, хемокінові рецептори Cxcr4 (C-X-C chemokine recept… Show more

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Cited by 3 publications

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Evaluation of the Complex Use of Plant Test-Systems to Determine the Nature and Intensity of the Mutagenic Background of the Environment

Shvets,

Frych,

Dovganych

et al. 2023

Polski Merkuriusz Lekarski

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Aim: A comprehensive evaluation of the effectiveness of the use of plant test-systems as biological objects for the identification of the specifitown of the effect of mutagens on the hereditary apparatus. Materials and methods: With the use of test-systems (determination of the gonadotoxitown of plant pollen grains, the Allium cepa-test and the use of celandine greater Chelidonium majus as an accumulative phytoindicator) was analyzed the ecological situation of the areas in Ivano-Frankivsk region. The samples of soil, water, generative and vegetative organs of plants were taken in the territories of the Carpathian region and tested in the following areas: ecologically safe, conditionally ecologically safe and ecologically dangerous areas. Results: Inhibition of mitotic activity in cells of the primary meristem of Allium cepa roots grown in water samples taken in chemically polluted Kalush and Burshtyn and stimulation of mitosis in water samples from Stetseva, was determined. The town Kosiv is considered conditionally ecologically clean, a control district. The indices of chromosomal aberrations frequency were high in plants from the chemically polluted Burshtyn (3.98±0.31) %, Kalush (2.95±0.25) % and village Stetseva (3.36±0.39) %, and dominated by controls 4.57-fold; 2.92-fold and 3.86-fold, respectively. Conclusions: With the help of a comprehensive study of two test-systems (determination of the fertility of plant reproductive structures and the Allium cepa test), we have proven an increased level of xenobiotics in the water, soil and air of the chemically polluted territoties of Precarpathian region. The use of Chelidonium majus as a test-object with metal-accumulating properties is a promising index for the detection of substances of a chemical nature (heavy metals) in ecological areas of the Ivano-Frankivsk region.

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Evaluation of the Complex Use of Plant Test-Systems to Determine the Nature and Intensity of the Mutagenic Background of the Environment

Shvets,

Frych,

Dovganych

et al. 2023

Polski Merkuriusz Lekarski

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Experimental Gestational Diabetes Disrupts the Formation of Immune Tolerance in Offspring

et al. 2023

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The aim: To analyze the mRNA gene expression level of Aire, Deaf1, Foxp3, Ctla4, Il10, Nlrp3 and distribution of NLRP3+-cells in mesenteric lymph nodes (MLNs) of the offspring of rats with GD, both untreated and treated with glibenclamide and in conditions of insulin oral tolerance formation. Materials and methods: The study involves 160 male rats, one- or six-month-old. The mRNA genes expression was studied by real time quantitative poly¬merase chain reaction. Structure of Nlrp3+ -cells population was studied by histological sections of MLNs. Results: We observed AIRE gene repression, reduced mRNA levels of Deaf1 and the transcription factor Foxp3 in offspring of rats with GD. This was accompanied by inhibition of IL-10 gene expression and negative costimulatory molecules Ctla4. The development of the experimental GD was accompanied by transcrip¬tional induction of the Nlrp3 gene in MLNs of descendants. The administration of glibenclamide to pregnant female rats with GD inhibited the transcription of the Nlrp3 gene only in one-month-old offspring (5.3-fold) and did not change it in six-month-old animals. In offspring of rats with GD, the density of the NLRP3+-lymphocyte population in the MLNs increased, more pronounced in one-month-old animals. The administration of glibenclamide to pregnant rats with GD reduced the number of NLRP3+ -lymphocytes only in one-month-old offspring (by 33.0 %), whereas this index in six month-old offspring even increased. Conclusions: Experimental prenatal hyperglycemia leads to increased proinflammatory signaling and violation of peripheral immunological tolerance formation more pronounced at one month of life.

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Transcriptional Activity of Neurotrophins Genes and Their Receptors in the Peripheral Blood in Patients with Thyroid Diseases in Bukovinian Population of Ukraine

Kamyshna

Kamyshnyі

2021

Open Access Maced J Med Sci

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Objective. Thyroid hormone has an especially strong impact on central nervous system development, and thyroid hormone deﬁciency has been shown to result in severe mental retardation. It is crucial to identify compensatory mechanisms that can be involved in improving cognitive function and the quality of life of patients with hypothyroidism. Methods: We used the pathway-specific PCR array (Neurotrophins and Receptors RT2 Profiler PCR Array, QIAGEN, Germany) to identify and validate neurotrophins genes and their receptor expression in patients with thyroid pathology and control group. Results: The analysis of gene expression of neurotrophins and their receptors showed that CRHBP, FRS2, FRS3, GFRA1, GFRA2, GMFB, NGF, NRG2, NRG4, NTF4, TRO, and VGF significantly decreased their expression in Group 3, which includes the patients with postoperative hypothyroidism. The patients with primary hypothyroidism stemming from AIT had significantly reduced expression of CRHBP, GFRA1, GFRA2, GMFB, NGF, PTGER2, and VGF, while the expression of NRG4 and TRO increased. In Group 3, which includes the patients with AIT and elevated serum anti-Tg and anti-TPO autoantibodies, the mRNA levels of GFRA2, NGF, NRG2, NTF4, NGF, PTGER were reduced, and the expression of CRHBP, FRS2, FRS3 GFRA1, GMFB, NRG4, TRO, and VGF significantly increased. Conclusion: These results indicate significant variability in the transcriptional activity of the genes of encoding neurotrophins and their receptors in the peripheral blood in people with thyroid diseases.

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Transcriptional Induction of Regulatory Genes Madcam1, S1pr1, Cxcr4 and Ccr7 that Control Lymphocyte Recirculation and Homing in Terms of Experimental Gestational Diabetes Affects T-cells Distribution in Mesenteric Lymph Nodes in Offspring

Cited by 3 publications

References 23 publications

Evaluation of the Complex Use of Plant Test-Systems to Determine the Nature and Intensity of the Mutagenic Background of the Environment

Evaluation of the Complex Use of Plant Test-Systems to Determine the Nature and Intensity of the Mutagenic Background of the Environment

Experimental Gestational Diabetes Disrupts the Formation of Immune Tolerance in Offspring

Transcriptional Activity of Neurotrophins Genes and Their Receptors in the Peripheral Blood in Patients with Thyroid Diseases in Bukovinian Population of Ukraine

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