Transcriptional profiling of contrasting genotypes revealed key candidates and nucleotide variations for drought dissection in Camellia sinensis (L.) O. Kuntze

Parmar, Rajni; Seth, Romit; Singh, Pradeep; Singh, Gopal; Kumar, Sanjay; Sharma, Ram Kumar

doi:10.1038/s41598-019-43925-w

Cited by 37 publications

(32 citation statements)

References 61 publications

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“…Similarly, F mean minimum latency for lower limbs was 50.66±4.72ms. 5 These findings are little higher than our values of F minimum latencies for upper and lower limbs. respectively.…”

Section: Discussioncontrasting

confidence: 67%

The F waves study in young healthy individuals

et al. 2018

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Background: The F wave is a CMAP (compound muscle action potential) evoked by a supramaximal stimulation of a motor nerve. F waves are particularly useful for the diagnoses of polyneuropathies at an early stage and proximal nerve lesions.Methods: Healthy males (n=64) and females (n=26) medical students of BPKIHS with age 20 to 24 years were enrolled. Anthropometric parameters; F wave latencies, persistence and chronodispersion of bilateral median, ulnar and tibial nerves were recorded in Neurophysiology Lab II of BPKIHS. Descriptive analysis was done.Results: Mean age, height and weight of the subjects were 21.64±1.19 years, 165.61±5.4cms and 64.07±5.5kg. Mean minimum F wave latencies (ms) of right median, ulnar and tibial nerves were 24.09±1.95, 24.02±1.76, 44.34±3.02 while on the left side were 23.92±1.96, 24.11±1.92, 44.07±2.83 respectively. F persistence was above 80%. F chronodispersion (ms) for right and left median, ulnar and tibial nerves were 2.77±0.70, 2.79±0.65, 2.71±0.67, 2.80±0.56, 3.48±0.73 and 3.45±0.64 respectively.Conclusions: Maximum and minimum F wave latencies, F chronodispersion and F persistence were derived for both sexes in an age group of 20-24 years.

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“…Similarly, F mean minimum latency for lower limbs was 50.66±4.72ms. 5 These findings are little higher than our values of F minimum latencies for upper and lower limbs. respectively.…”

Section: Discussioncontrasting

confidence: 67%

The F waves study in young healthy individuals

et al. 2018

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“…These genes were reported to be upregulated in shallot doubled haploid and might have imparted stress tolerance to shallot than to onion [ 11 ]. Carbohydrate metabolism-associated genes were also upregulated in onion under cold stress [ 12 ] and in Camellia sinensis under drought stress [ 9 ].…”

Section: Discussionmentioning

confidence: 99%

Comparative transcriptome analyses in contrasting onion (Allium cepa L.) genotypes for drought stress

et al. 2020

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Onion ( Allium cepa L.) is an important vegetable crop widely grown for diverse culinary and nutraceutical properties. Being a shallow-rooted plant, it is prone to drought. In the present study, transcriptome sequencing of drought-tolerant (1656) and drought-sensitive (1627) onion genotypes was performed to elucidate the molecular basis of differential response to drought stress. A total of 123206 and 139252 transcripts (average transcript length: 690 bases) were generated after assembly for 1656 and 1627, respectively. Differential gene expression analyses revealed upregulation and downregulation of 1189 and 1180 genes, respectively, in 1656, whereas in 1627, upregulation and downregulation of 872 and 1292 genes, respectively, was observed. Genes encoding transcription factors, cytochrome P450, membrane transporters, and flavonoids, and those related to carbohydrate metabolism were found to exhibit a differential expression behavior in the tolerant and susceptible genotypes. The information generated can facilitate a better understanding of molecular mechanisms underlying drought response in onion.

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“…Due to out-breeding and its long gestation period, the tea plant requires next-generation breeding strategies to improve its drought and cold tolerance through a deeper understanding of key regulators and their variants for precision introgressions to have better yield and quality under stress conditions. Therefore, efforts are needed to elucidate the global transcriptomic dynamics of multiple tea genotypes in drought and cold stress to critically discern key molecular players (Parmar et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Comparative Expression Analysis of Stress-Inducible Candidate Genes in Response to Cold and Drought in Tea Plant [Camellia sinensis (L.) Kuntze]

et al. 2020

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Cold and drought are two of the most severe threats affecting the growth and productivity of the tea plant, limiting its global spread. Both stresses cause osmotic changes in the cells of the tea plant by decreasing their water potential. To develop cultivars that are tolerant to both stresses, it is essential to understand the genetic responses of tea plant to these two stresses, particularly in terms of the genes involved. In this study, we combined literature data with interspecific transcriptomic analyses (using Arabidopsis thaliana and Solanum lycopersicum) to choose genes related to cold tolerance. We identified 45 stress-inducible candidate genes associated with cold and drought responses in tea plants based on a comprehensive homologous detection method. Of these, nine were newly characterized by us, and 36 had previously been reported. The gene network analysis revealed upregulated expression in ICE1-related cluster of bHLH factors, HSP70/BAM5 connected genes (hexokinases, galactinol synthases, SnRK complex, etc.) indicating their possible co-expression. Using qRT-PCR we revealed that 10 genes were significantly upregulated in response to both cold and drought in tea plant: HSP70, GST, SUS1, DHN1, BMY5, bHLH102, GR-RBP3, ICE1, GOLS1, and GOLS3. SnRK1.2, HXK1/2, bHLH7/43/79/93 were specifically upregulated in cold, while RHL41, CAU1, Hydrolase22 were specifically upregulated in drought. Interestingly, the expression of CIP was higher in the recovery stage of both stresses, indicating its potentially important role in plant recovery after stress. In addition, some genes, such as DHN3, bHLH79, PEI54, SnRK1.2, SnRK1.3, and Hydrolase22, were significantly positively correlated between the cold and drought responses. CBF1, GOLS1, HXK2, and HXK3, by contrast, showed significantly negative correlations between the cold and drought responses. Our results provide valuable information and robust candidate genes for future functional analyses intended to improve the stress tolerance of the tea plant and other species.

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Transcriptional profiling of contrasting genotypes revealed key candidates and nucleotide variations for drought dissection in Camellia sinensis (L.) O. Kuntze

Cited by 37 publications

References 61 publications

The F waves study in young healthy individuals

The F waves study in young healthy individuals

Comparative transcriptome analyses in contrasting onion (Allium cepa L.) genotypes for drought stress

Comparative Expression Analysis of Stress-Inducible Candidate Genes in Response to Cold and Drought in Tea Plant [Camellia sinensis (L.) Kuntze]

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